为探讨黄腐酸提高大豆结瘤能力的作用机理,以辽宁慢生根瘤菌(Bradyrhizobium liaoningense)CCBAU 05525和冀豆17为材料,采用高效液相色谱法(HPLC)探究黄腐酸对大豆结瘤初期内源激素含量、分布及结瘤的影响.结果表明:黄腐酸质量浓度为500 mg/L时大豆结瘤数量最多,比对照组显著提高73.1%,同时该条件下激素含量和分布变化最为明显,具体表现为大豆根、茎、叶中玉米素(ZT)、生长素(IAA)、赤霉素(GA3)含量增加,根中脱落酸(ABA)含量减少,叶中ABA含量增加.而黄腐酸质量浓度为1 000 mg/L时则表现为抑制大豆结瘤. 上述结果为解析黄腐酸提高大豆结瘤固氮的机理、增加大豆产量提供了理论依据.
In order to explore the mechanism of fulvic acid improving soybean nodulation ability, Bradyrhizobium liaoningense CCBAU 05525 and Jidou 17 were used to evaluate the effects of fulvic acid on the content and distribution of endogenous hormones by HPLC and on nodule number at the early stage of soybean nodulation. The results showed that when the concentration of fulvic acid was 500 mg/L, the number of soybean nodules was increased significantly by 73.1% at most, and the change of hormone content and distribution was the most obvious. The specific performance was that the content of zeatin(ZT), auxin(IAA)and gibberellin(GA3)in root, stem and leaf increased, the content of abscisic acid(ABA)in root decreased, and the content of ABA in leaf increased. Fulvic acid at 1 000 mg/L inhibited soybean nodulation. These results provide a theoretical basis for analyzing the mechanism of fulvic acid improving nodulation and nitrogen fixation of soybean and increasing soybean yield.
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