当pH=5.2时茜素红与过量的Al3+反应生成发射橙黄色荧光的络合物,该络合物的最佳激发和发射波长分别为469 nm和585 nm,Cu2+能够使茜素红-铝体系荧光猝灭,且荧光猝灭程度与Cu2+的浓度在一定范围内呈线性关系,据此建立了一种灵敏的“开关式”测定Cu2+的方法.探究了茜素红-铝-铜体系的最佳用量比、反应温度、反应时间、pH、缓冲溶液等因素对反应体系的影响.结果表明:测定Cu2+的工作曲线为 ΔF=19.7c+101.2,R2=0.999 5,线性为0.2~30 μmol/L,检出限0.022 μmol/L.该方法准确度高,灵敏度高,选择性好,可用于水中微量铜的检测.
A complex emitting orange yellow fluorescence were generated by the mixture of the alizarin red and excessive Al3+ at pH = 5.2. The maximum fluorescence excitation and emission wavelength of the colored complex were located at 469 nm and at 585 nm, respectively, The fluorescence intensity of the alizarin red-Al3+ system could be quenched by a certain amount of Cu2+,and the fluorescence quenching value showed a linear relationship with the concentration of Cu2+ within limits, thus establishing a sensitive and switching method for the determination of Cu2+. The optimal factors of the determination system were investigated,such as the dosage ratio, reaction temperature, reaction time,pH and buffer solution. The results showed that the standard working curve was ΔF=19.7c+101.21(R2=0.999 5), the linear range was 0.2~30 μmol/L, and the detection limit was 0.022 μmol/L. The method shows high accuracy, high sensitivity, good selectivity, and could be used for the detection of trace copper ion in water.
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