为减少标准汤剂和配方颗粒制备过程中挥发油的损失,将挥发油与β-环糊精(β-CD)包合,以挥发油的包合率为评价指标,优化艾叶挥发油与β-CD的包合工艺,并采用气相色谱法测定包合率.通过指标成分桉油精和龙脑的含量测定及指纹图谱考查饮片、标准汤剂、配方颗粒的相关性.最终确定最佳包合条件,所得挥发油平均包合率为96.40%,建立了艾叶标准汤剂和配方颗粒的制备方法,制得的配方颗粒中桉油精的平均质量分数0.738 4 mg/g,转移率33.48%,龙脑的平均质量分数0.334 1 mg/g,转移率25.41%.所建立包合工艺和配方颗粒制备工艺稳定性高,重复性好,标准汤剂和配方颗粒在指标成分含量上具有较好的一致性,可为企业工业化生产提供理论参考.
In order to reduce the loss of volatile was oil in the preparation of standard decoction and formula granules, the volatile oil was included with β-cyclodextrin(β-CD), and the inclusion rate of volatile oil was used as an evaluation index to optimize the package of volatile oil and β-CD. Gas chromatography was used to determine the inclusion rate. The correlation among decoction pieces, standard decoction and formula granules was investigated by the content determination of index components and fingerprint. The average inclusion rate of volatile oil obtained by inclusion process was 96.40%, and the preparation method of Artemisia argyi standard decoction and formula granules was established. The average content of eucalyptus olein in the formula granules was 0.738 4 mg/g, and the transfer rate was 33.48%. The average content of borneol was 0.334 1 mg/g, and the transfer rate was 25.41%. The inclusion process and the preparation process of formula granules have high stability and good repeatability.The content of index components of standard decoction and formula granules is consistent, which can provide theoretical reference for industrial production of enterprises.
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