为获得高产优质的黑木耳菌株,以黑木耳(Auricularia auricula)菌株Aa66为出发菌株,采用多功能等离子体诱变系统(multifunction plasma mutagenesis system,MPMS)进行诱变育种实验,共获得315株诱变菌株.通过初筛拮抗实验及复筛对菌株长速长势、酶活及生物学效率的测定,共得到8株优良诱变菌株,且使用简单重复序列区间扩增多态性分子标记技术(inter-simple sequence repeat,ISSR)在分子水平上确定其均与出发菌株Aa66有明显差异,其中优良诱变菌株Y9生长速度最快,为4.98 mm/d,较出发菌株Aa66提高0.54 mm/d,生物学效率为115.34%,较出发菌株Aa66提高8.43%,表明MPMS可以作为一种选育优良黑木耳菌株的新方法.
In order to obtain high-yield and high-quality Auricularia auricula strains, using A. auricula strain Aa66 as the starting strain, multifunction plasma mutagenesis system(MPMS)was used to carry out mulation breeding experiments, and a total of 315 mutant strains were obtained. The antagonism experiment and re-screening were used to determine the growth rate, enzyme activity and biological efficiency of the strain. Through the preliminary screening antagonistic experiment and the determination of strain growth rate,growth trend,enzyme activity and biological efficiency by re-screening, a total of 8 excellent mutagenic strains were obtained, and the inter-simple sequence repeat(ISSR)technology was used to amplify polymorphic molecular markers. They were determined to be significantly different from the starting strain Aa66 at the molecular level. Among them, the excellent mutant strain Y9 grew with the fastest speed of 4.98 mm/d, which was 0.54 mm/d higher than that of the starting strain Aa66, and the biological efficiency was 115.34%, which was 8.43% higher than that of the starting strain Aa66, indicating that MPMS can be used as a new method for breeding excellent A. auricula strains. - DOI:10.3969/j.issn.1000-1565.2022.06.013基于MPMS方法的黑木耳优良菌株选育尹显达1,刘敏1,杨超上1,张铁军2,王谦1(1.河北大学 生物工程技术创新中心,河北 保定 071002;2.承德市农业环境保护监测站,河北 承德 067000)摘 要:为获得高产优质的黑木耳菌株,以黑木耳(Auricularia auricula)菌株Aa66为出发菌株,采用多功能等离子体诱变系统(multifunction plasma mutagenesis system,MPMS)进行诱变育种实验,共获得315株诱变菌株.通过初筛拮抗实验及复筛对菌株长速长势、酶活及生物学效率的测定,共得到8株优良诱变菌株,且使用简单重复序列区间扩增多态性分子标记技术(inter-simple sequence repeat,ISSR)在分子水平上确定其均与出发菌株Aa66有明显差异,其中优良诱变菌株Y9生长速度最快,为4.98 mm/d,较出发菌株Aa66提高0.54 mm/d,生物学效率为115.34%,较出发菌株Aa66提高8.43%,表明MPMS可以作为一种选育优良黑木耳菌株的新方法.关键词:黑木耳;多功能等离子体诱变系统(MPMS);诱变;分子标记中图分类号:S646.6 文献标志码:A 文章编号:1000-1565(2022)06-0650-07Breeding of fine strains of Auricularia auriculata based on MPMS methodYIN Xianda1, LIU Min1, YANG Chaoshang1, ZHANG Tiejun2, WANG Qian1(1.Bioengineering Technology Innovation Center, Hebei University, Baoding 071002, China; 2.Chengde City Agricultural Environmental Protection Monitoring Station, Chengde 067000, China)Abstract: In order to obtain high-yield and high-quality Auricularia auricula strains, using A. auricula strain Aa66 as the starting strain, multifunction plasma mutagenesis system(MPMS)was used to carry out mulation breeding experiments, and a total of 315 mutant strains were obtained. The antagonism experiment and re-screening were used to determine the growth rate, enzyme activity and biological efficiency of the strain. Through the preliminary screening antagonistic experiment and the determination of strain growth rate,growth trend,enzyme activity and biological efficiency by re-screening, a total of 8 excellent mutagenic strains were obtained, and the inter-simple sequence repeat(ISSR)technology was used to amplify polymorphic molecular markers. They were determined to be significantly different from the starting strain Aa66 at the molecular level. Among them, the excellent mutant strain Y9 grew with the fastest speed of 4.98 mm/d, which was 0.54 mm/d higher than that of the starting strain Aa66, and the biological efficiency was 115.34%, which was 8.43% higher than that of the starting strain Aa66, indicating that MPMS can be used as a new method for breeding excellent A. auricula strains. - 收稿日期:2022-04-28 基金项目:河北省现代农业食用菌产业技术体系创新团队(第2期)(HBCT2018050204) 第一作者:尹显达(1996—),男,河北廊坊人,河北大学在读硕士研究生,主要从事大型真菌研究.E-mail:yin19965@sina.com 通信作者:王谦(1962—),男,北京人,河北大学研究员,主要从事食药用真菌研究与开发工作.E-mail:wq6203_cn@126.com第6期尹显达等:基于MPMS方法的黑木耳优良菌株选育
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