为了研究乙醛脱氢酶ALDH2基因敲除对雄性小鼠发育的影响,将不同周龄ALDH2基因敲除的雄性小鼠,通过分析其肝、肾、睾丸脏器质量分数,组织细胞形态,组织抗氧化能力,总产仔数等指标,探讨ALDH2基因敲除对雄性小鼠的影响.结果表明:7、10周龄敲除型(KO)小鼠肝细胞索排列紊乱,10周龄时,多核肝细胞数量明显增多,且肝细胞中黑色沉积物增加;7、10周龄KO小鼠肾脏出现明显的间质水肿;7、10周龄KO小鼠睾丸生精管内晚期精细胞和精子排序紊乱,腔内有脱落的生精细胞,睾丸组织出现纤维化;10周龄KO小鼠肝脏、肾脏总抗氧化能力和3、5周龄KO小鼠睾丸组织总抗氧化能力显著低于野生型(WT)小鼠;KO组小鼠产仔总数显著少于WT小鼠.综上,ALDH2敲除导致小鼠肝脏、肾脏和睾丸组织抗氧化损伤的能力降低,造成其细胞氧化损伤,进而影响雄性小鼠的生育力.该研究结果说明ALDH2基因敲除会引发雄性小鼠组织器官发育异常,进而影响小鼠的个体发育.
In order to study the effects of ALDH2 gene knockout on the development of male mice, the effects of ALDH2 gene knockout on male mice were studied by analyzing the organ mass fraction of liver, kidney and testis, histomorphology, tissue antioxidant capacity and total litter size of mice at different weeks of age. The results showed that the cords of hepatocytes of Knockout type(KO)mice at 7 and 10 weeks of age were disordered. At 10 weeks of age, the number of multinucleated hepatocytes, and the black deposits in hepatocytes increased significantly. The kidneys of KO mice at 7 and 10 weeks of age showed obvious interstitial edema. In 7 and 10 weeks of aged mice, the sequence of late spermatogenic cells and spermatogenic cells in testicular seminiferous tubules were disordered. The total antioxidant capacity of liver and kidney in 10 weeks of age KO mice and the total antioxidant capacity of testis in 3 and 5 weeks of age KO mice were significantly lower than that of wild-type(WT)mice. The total number of pups in KO group was significantly less than that of WT mice. In conclusion, ALDH2 knockout can reduce the ability of anti-oxidative damage in liver, kidney and testis tissue of mice, result in cell damage, and affect the fertility of male mice in turn.The results showed that ALDH2 gene knockout would cause- DOI:10.3969/j.issn.1000-1565.2023.05.008乙醛脱氢酶ALDH2基因敲除对雄性小鼠发育的影响徐文秀,刘庆玲,刘笑含,孔令英,石慧,赵振军(烟台大学 生命科学学院,山东 烟台 264003)摘 要:为了研究乙醛脱氢酶ALDH2基因敲除对雄性小鼠发育的影响,将不同周龄ALDH2基因敲除的雄性小鼠,通过分析其肝、肾、睾丸脏器质量分数,组织细胞形态,组织抗氧化能力,总产仔数等指标,探讨ALDH2基因敲除对雄性小鼠的影响.结果表明:7、10周龄敲除型(KO)小鼠肝细胞索排列紊乱,10周龄时,多核肝细胞数量明显增多,且肝细胞中黑色沉积物增加;7、10周龄KO小鼠肾脏出现明显的间质水肿;7、10周龄KO小鼠睾丸生精管内晚期精细胞和精子排序紊乱,腔内有脱落的生精细胞,睾丸组织出现纤维化;10周龄KO小鼠肝脏、肾脏总抗氧化能力和3、5周龄KO小鼠睾丸组织总抗氧化能力显著低于野生型(WT)小鼠;KO组小鼠产仔总数显著少于WT小鼠.综上,ALDH2敲除导致小鼠肝脏、肾脏和睾丸组织抗氧化损伤的能力降低,造成其细胞氧化损伤,进而影响雄性小鼠的生育力.该研究结果说明ALDH2基因敲除会引发雄性小鼠组织器官发育异常,进而影响小鼠的个体发育.关键词:乙醛脱氢酶2;肝脏;肾脏;睾丸;组织抗氧化;生育力中图分类号:Q954.6 文献标志码:A 文章编号:1000-1565(2023)05-0500-06Effects of ALDH2 gene knockout on the development of male miceXU Wenxiu,LIU Qingling,LIU Xiaohan,KONG Lingying,SHI Hui,ZHAO Zhenjun(School of Life Sciences,Yantai University,Yantai 264003,China)Abstract: In order to study the effects of ALDH2 gene knockout on the development of male mice, the effects of ALDH2 gene knockout on male mice were studied by analyzing the organ mass fraction of liver, kidney and testis, histomorphology, tissue antioxidant capacity and total litter size of mice at different weeks of age. The results showed that the cords of hepatocytes of Knockout type(KO)mice at 7 and 10 weeks of age were disordered. At 10 weeks of age, the number of multinucleated hepatocytes, and the black deposits in hepatocytes increased significantly. The kidneys of KO mice at 7 and 10 weeks of age showed obvious interstitial edema. In 7 and 10 weeks of aged mice, the sequence of late spermatogenic cells and spermatogenic cells in testicular seminiferous tubules were disordered. The total antioxidant capacity of liver and kidney in 10 weeks of age KO mice and the total antioxidant capacity of testis in 3 and 5 weeks of age KO mice were significantly lower than that of wild-type(WT)mice. The total number of pups in KO group was significantly less than that of WT mice. In conclusion, ALDH2 knockout can reduce the ability of anti-oxidative damage in liver, kidney and testis tissue of mice, result in cell damage, and affect the fertility of male mice in turn.The results showed that ALDH2 gene knockout would cause- 收稿日期:2022-10-20 基金项目:国家重点研发计划(2018YFC1003600) 第一作者:徐文秀(1996—),女,山东临沂人,烟台大学在读硕士研究生,主要从事雄性小鼠生殖发育研究.E-mail:xwx5935@163.com 通信作者:赵振军(1976—),男,山东烟台人,烟台大学副教授,博士,主要从事小鼠生殖发育研究. E-mail:zhaozhenjun@ytu.edu.cn第5期徐文秀等:乙醛脱氢酶ALDH2基因敲除对雄性小鼠发育的影响abnormal development of tissues and organs in male mice, and then affect the individual development of mice.
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