河北大学学报(自然科学版) >

2024 , Vol. 44 >Issue 1: 84 - 91

DOI: https://doi.org/10.3969/j.issn.1000-1565.2024.01.011

微塑料的测定方法以及对水生生物的生态毒理效应

  • 崔铁峰 ,
  • 张杰 ,
  • 卢灿然 ,
  • 王宏伟 ,
  • 叶晔
展开
崔铁峰(1995—),男,河北大学硕士研究生,华东师范大学在读博士研究生,主要从事生态学研究.
E-mail: 2413829127@qq.com

收稿日期: 2022-11-01

  网络出版日期: 2024-03-15

基金资助

农业农村部项目(2130135);教育部留学回国基金资助项目(201808130270);河北省自然科学基金创新研究群体项目(C2022201042);河北省科技特派员项目(2022-2024)

收起

Methods for determination of microplastics and their ecotoxicological effects on aquatic organisms

  • CUI Tiefeng ,
  • ZHANG Jie ,
  • LU Canran ,
  • WANG Hongwei ,
  • YE Ye
Expand
  • 1. Institute of Life Science and Green Development, College of Life Sciences, Hebei University, Baoding 071002, China; 2. State Key Laboratory of Estuarine and Coastal Science, Institute of Estuarine and Coastal Sciences, East China Normal University, Shanghai 200241, China; 3.Science and Technology Center, China-Japan Friendship and Environmental Protection Center, Beijing 100029, China

Received date: 2022-11-01

  Online published: 2024-03-15

Fold

摘要

综述了当前微塑料(microplastics, MPs)分离和分析方法的研究进展, 包括机械过滤、化学处理、胶体共沉淀、密度梯度离心和光学/荧光/拉曼分析等方法, 同时介绍了它们的优缺点和适用范围, 为微塑料污染的检测、监测和评估提供了参考.概述了MPs对水生生物的毒性效应:MPs在被水生生物摄入的过程中会对生物体的消化道造成物理损伤, 同时MPs的摄入会增加饱腹感, 影响水生生物对食物的消化吸收;MPs的摄入还会使水生生物肠道中的微生物群落组成和多样性发生改变;MPs暴露会引起水生生物基因的改变,还会诱发水生生物的氧化应激反应, 并干扰其脂质和能量代谢.MPs对消化系统的影响及对肠道微生物平衡的破坏可能是生物体脂质和能量代谢相关基因改变的原因之一.MPs可以吸附和富集其他物质, 从而对生物产生更严重的联合毒理效应.MPs对生物体消化道的物理损伤及其在生物体内的积累和扩散可能是引起氧化应激反应和免疫反应的主要原因.不仅对水生生物, MPs对人类的健康风险也引起人们对其潜在影响的关注.

关键词： 微塑料; 水生生物; 生态毒理效应

本文引用格式

崔铁峰 , 张杰 , 卢灿然 , 王宏伟 , 叶晔 . 微塑料的测定方法以及对水生生物的生态毒理效应[J]. 河北大学学报(自然科学版), 2024 , 44(1) : 84 -91 . DOI: 10.3969/j.issn.1000-1565.2024.01.011

Abstract

The research progress of separation and analysis methods of Microplastics(MPs), including mechanical filtration, chemical treatment, colloidal coprecipitation, density gradient centrifugation and optical/fluorescence/Raman analysis, was reviewed. Their advantages, disadvantages and application range- DOI:10.3969/j.issn.1000-1565.2024.01.011微塑料的测定方法以及对水生生物的生态毒理效应崔铁峰1,2,张杰1,卢灿然1,王宏伟1,叶晔3(1.河北大学 生命科学学院 生命科学与绿色发展研究院,河北 保定 071002;2.华东师范大学 河口海岸科学研究院,河口海岸国家重点实验室,上海 200241;3.中日友好环境保护中心 科技中心,北京 100029)摘 要:综述了当前微塑料(microplastics, MPs)分离和分析方法的研究进展, 包括机械过滤、化学处理、胶体共沉淀、密度梯度离心和光学/荧光/拉曼分析等方法, 同时介绍了它们的优缺点和适用范围, 为微塑料污染的检测、监测和评估提供了参考.概述了MPs对水生生物的毒性效应:MPs在被水生生物摄入的过程中会对生物体的消化道造成物理损伤, 同时MPs的摄入会增加饱腹感, 影响水生生物对食物的消化吸收;MPs的摄入还会使水生生物肠道中的微生物群落组成和多样性发生改变;MPs暴露会引起水生生物基因的改变,还会诱发水生生物的氧化应激反应, 并干扰其脂质和能量代谢.MPs对消化系统的影响及对肠道微生物平衡的破坏可能是生物体脂质和能量代谢相关基因改变的原因之一.MPs可以吸附和富集其他物质, 从而对生物产生更严重的联合毒理效应.MPs对生物体消化道的物理损伤及其在生物体内的积累和扩散可能是引起氧化应激反应和免疫反应的主要原因.不仅对水生生物, MPs对人类的健康风险也引起人们对其潜在影响的关注.关键词:微塑料;水生生物;生态毒理效应中图分类号:Q89 文献标志码:A 文章编号:1000-1565(2024)01-0084-08Methods for determination of microplastics and their ecotoxicological effects on aquatic organismsCUI Tiefeng1, 2, ZHANG Jie1, LU Canran1, WANG Hongwei1, YE Ye3(1. Institute of Life Science and Green Development,College of Life Sciences,Hebei University,Baoding 071002,China;2. State Key Laboratory of Estuarine and Coastal Science, Institute of Estuarine and Coastal Sciences, East China Normal University, Shanghai 200241, China;3.Science and Technology Center,China-Japan Friendship and Environmental Protection Center, Beijing 100029, China)Abstract: The research progress of separation and analysis methods of Microplastics(MPs), including mechanical filtration, chemical treatment, colloidal coprecipitation, density gradient centrifugation and optical/fluorescence/Raman analysis, was reviewed. Their advantages, disadvantages and application range- 收稿日期:2022-11-01;修回日期:2023-08-14 基金项目:农业农村部项目(2130135);教育部留学回国基金资助项目(201808130270);河北省自然科学基金创新研究群体项目(C2022201042);河北省科技特派员项目(2022-2024) 第一作者:崔铁峰(1995—),男,河北大学硕士研究生,华东师范大学在读博士研究生,主要从事生态学研究.E-mail: 2413829127@qq.com 通信作者:王宏伟(1970—),女,河北大学教授,博士,主要从事水生生物环境监测与保护研究. E-mail: whw66668888@126.com叶晔(1980—),女,中日友好环境保护中心高级工程师,主要从事水生态健康评估研究.E-mail:ye200506@163.com第1期崔铁峰等:微塑料的测定方法以及对水生生物的生态毒理效应were also introduced, which provided a reference for the detection, monitoring and evaluation of microplastics pollution. The toxic effects of MPs on aquatic organisms were also reviewed: MPs could cause physical damage to the digestive tract of aquatic organisms during ingestion, while the ingestion of MPs could increase satiety and affect the digestion and absorption of food by aquatic organisms; the ingestion of MPs could also alter the composition and diversity of microbial communities in the gut of aquatic organisms; MPs exposure could cause genetic alterations in aquatic organisms. The effects of MPs on the digestive system and the disruption of gut microbial homeostasis may be one of the causes of genetic alterations related to lipid and energy metabolism in organisms; the physical damage to the digestive tract and the accumulation and spread of MPs in organisms may be the main cause of oxidative stress and immune responses. MPs could adsorb and enrich other substances, resulting in more severe combined toxicological effects on organisms.The physical damage to the digestive tract of organisms and their accumulation and spread in the organism may be the main cause of oxidative stress and immune response.

Key words： microplastics; aquatic organisms; ecotoxicological effects

参考文献

[1] LARES M, NCIBI M C, SILLANPÄÄ M, et al. Occurrence, identification and removal of microplastic particles and fibers in conventional activated sludge process and advanced MBR technology[J]. Water Res, 2018, 133: 236-246. DOI: 10.1016/j.watres.2018.01.049.
[2] ROCHA-SANTOS T, DUARTE A C. A critical overview of the analytical approaches to the occurrence, the fate and the behavior of microplastics in the environment[J]. Trac Trends Anal Chem, 2015, 65: 47-53. DOI: 10.1016/j.trac.2014.10.011.
[3] BARNES D K A, GALGANI F, THOMPSON R C, et al. Accumulation and fragmentation of plastic debris in global environments[J]. Philos Trans R Soc Lond B Biol Sci, 2009, 364(1526): 1985-1998. DOI: 10.1098/rstb.2008.0205.
[4] LI C J, WANG X H, LIU K, et al. Pelagic microplastics in surface water of the Eastern Indian Ocean during monsoon transition period: abundance, distribution, and characteristics[J]. Sci Total Environ, 2021, 755: 142629. DOI: 10.1016/j.scitotenv.2020.142629.
[5] OBBARD R W, SADRI S, WONG Y Q, et al. Global warming releases microplastic legacy frozen in Arctic Sea ice[J]. Earths Future, 2014, 2(6): 315-320. DOI: 10.1002/2014ef000240.
[6] WATT E, PICARD M, MALDONADO B, et al. Ocean plastics: environmental implications and potential routes for mitigation-a perspective[J]. RSC Adv, 2021, 11(35): 21447-21462. DOI: 10.1039/d1ra00353d.
[7] CÓZAR A, ECHEVARRÍA F, GONZÁLEZ-GORDILLO J I, et al. Plastic debris in the open ocean[J]. Proc Natl Acad Sci USA, 2014, 111(28): 10239-10244. DOI: 10.1073/pnas.1314705111.
[8] KOLANDHASAMY P, SU L N, LI J, et al. Adherence of microplastics to soft tissue of mussels: a novel way to uptake microplastics beyond ingestion[J]. Sci Total Environ, 2018, 610/611: 635-640. DOI: 10.1016/j. scitotenv.2017.08.053.
[9] LI Q P, SUN C J, WANG Y J, et al. Fusion of microplastics into the mussel byssus[J]. Environ Pollut, 2019, 252: 420-426. DOI: 10.1016/j.envpol.2019.05.093.
[10] SU L, XUE Y G, LI L Y, et al. Microplastics in Taihu Lake, China[J]. Environ Pollut, 2016, 216: 711-719. DOI: 10.1016/j.envpol.2016.06.036.
[11] LI J N, QU X Y, SU L, et al. Microplastics in mussels along the coastal waters of China[J]. Environ Pollut, 2016, 214: 177-184. DOI: 10.1016/j.envpol.2016.04.012.
[12] VAN CAUWENBERGHE L, JANSSEN C R. Microplastics in bivalves cultured for human consumption[J]. Environ Pollut, 2014, 193: 65-70. DOI: 10.1016/j.envpol.2014.06.010.
[13] NABI I, BACHA A U R, ZHANG L, et al.A review on microplastics separation techniques from environmental media[J].J Clean Prod, 2022, 20(337):130458.DOI:10.1016/j.jclepro.2022.130458.
[14] YAHYANEZHAD N, BARDI M J, AMINIRAD H, et al.An evaluation of microplastics fate in the wastewater treatment plants: frequency and removal of microplastics by microfiltration membrane Uncorrected Proof[J]. Water Prac Technol, 2021, 3(16): 782-792. DOI:10.2166/wpt.2021.036.
[15] HURLEY R R, LUSHER A L, OLSEN M, et al.Validation of a method for extracting microplastics from complex, organic-rich, environmental matrices[J].Environ Sci Technol, 2018, 52(13):7409-7417.DOI:10.1021/acs.est.8b01517.
[16] TAKÁCS D, SZABÓ T, JAMNIK A, et al.Colloidal interactions of microplastic particles with anionic clays in electrolyte soutions[J].Langmuir, 2023, 39(36): 12835-12844.DOI: 10.1021/acs.langmuir.3c01700.
[17] YU X, PENG J, LI X, et al. Microplastics pollution in rivers: a review of the methods, occurrence, effects, and potential solutions[J]. Sci Total Environ, 2019, 630: 657.DOI: 10.1016/j.scitotenv.2018.12.382.
[18] SCHEURER M, BIGALKE M. Microplastics in swiss floodplain soils[J].Environ Sci Technol,2018, 52(6): 3591-3598.DOI: 10.1021/acs.est.7b06003.
[19] MBACHU O, JENKINS G, KAPARAJU P,et al.The rise of artificial soil carbon inputs: reviewing microplastic pollution effects in the soil environment[J].Sci Total Environ, 2021, 780(23):146569.DOI:10.1016/j.scitotenv.2021.146569.
[20] YANG D Q, SHI H H, LI L, et al. Microplastic pollution in table salts from China[J]. Environ Sci Technol, 2015, 49(22): 13622-13627. DOI: 10.1021/acs.est.5b03163.
[21] MOELLER J N, LOEDER M G J, LAFORSCH C, et al.Finding microplastics in soils: a review of analytical methods[J].Environ Sci Technol, 2020,54(4):2078-2090.DOI:10.1021/acs.est.9b04618.
[22] KUDELSKI A.Analytical applications of Raman spectroscopy[J].Talanta,2008,76(1):1-8.DOI:10.1016/j.talanta.2008.02.042.
[23] VOGT S, MASER J, JACOBSEN C, et al.Data analysis for X-ray fluorescence imaging[J].J Phys IV, 2002, 104:617-622.DOI:10.1051/jp4:20030156.
[24] HONG J, JIWEI R, CHENYANG J, et al.XRF combined with cluster analysis for inspection of pharmaceutical aluminum-plastic package[J]. New Chem Mater, 2019, 31(3):391-396.
[25] SIREGAR J P, SALIT M S, RAHMAN M Z A,et al.Thermogravimetric analysis(TGA)and differential scanning calometric(DSC)analysis of pineapple leaf fibre(PALF)reinforced high impact polystyrene(HIPS)composites[J]. Pertanika J Sci Technol, 2011, 19(1):161-170.
[26] EHRENSTEIN G W, RIEDEL G, TRAWIEL P.Thermal Analysis of Plastics[M]. Germany,München:Hanser Publishers,2004.
[27] 查旭琼,王娟,郑阳,等.土壤微塑料的检测方法研究进展[J].山东化工,2021,50(13):56-58.DOI:10.19319/j.cnki.issn.1008-021x.2021.13.024.
[28] 赵娜,孙月娜,时丽艳,等.化妆品塑料包装中16种增塑剂的迁移规律[J].河北大学学报(自然科学版), 2019, 39(3): 270-277. DOI: 10.3969/j. issn. 1000-1565.2019.03.008.
[29] DU C, WU J, GONG J, et al. ToF-SIMS characterization of microplastics in soils[J].Surf Interface Anal, 2020, 52(5):293-300.DOI:10.1002/sia.6742.
[30] NOLTE T M, HARTMANN N B, KLEIJN J M, et al. The toxicity of plastic nanoparticles to green algae as influenced by surface modification, medium hardness and cellular adsorption[J]. Aquat Toxicol, 2017, 183: 11-20. DOI: 10.1016/j.aquatox.2016.12.005.
[31] WATTS A J R, LEWIS C, GOODHEAD R M, et al. Uptake and retention of microplastics by the shore crab Carcinus maenas[J]. Environ Sci Technol, 2014, 48(15): 8823-8830. DOI: 10.1021/es501090e.
[32] SUMAN T Y, JIA P P, LI W G, et al. Acute and chronic effects of polystyrene microplastics on brine shrimp: first evidence highlighting the molecular mechanism through transcriptome analysis[J]. J Hazard Mater, 2020, 400: 123220. DOI: 10.1016/j.jhazmat.2020.123220.
[33] LU X, ZHANG J X, ZHANG L, et al. Negative impacts of chronic dietary exposure to polyethylene microplastics on growth, gut microbiota, liver metabolism and gene expressions in genetically improved farmed tilapia(Oreochromis niloticus)[J]. Sci Total Environ, 2022: 841: 156571. DOI: 10.1016/ j. scitotenv. 2022. 156571.
[34] TALVITIE J, HEINONEN M, PÄÄKKÖNEN J P, et al. Do wastewater treatment plants act as a potential point source of microplastics? Preliminary study in the coastal Gulf of Finland, Baltic Sea[J]. Water Sci Technol, 2015, 72(9): 1495-1504. DOI: 10.2166/wst.2015.360.
[35] JIN Y X, XIA J Z, PAN Z H, et al. Polystyrene microplastics induce microbiota dysbiosis and inflammation in the gut of adult zebrafish[J]. Environ Pollut, 2018, 235: 322-329. DOI: 10.1016/j.envpol.2017.12.088.
[36] HU J M, ZUO J E, LI J B, et al. Effects of secondary polyethylene microplastic exposure on crucian(Carassius carassius)growth, liver damage, and gut microbiome composition[J]. Sci Total Environ, 2022, 802: 149736. DOI: 10.1016/j.scitotenv.2021.149736.
[37] XIE M J, LIN L, XU P, et al. Effects of microplastic fibers on Lates calcarifer juveniles: accumulation, oxidative stress, intestine microbiome dysbiosis and histological damage[J]. Ecol Indic, 2021, 133: 108370. DOI: 10.1016/j.ecolind.2021.108370.
[38] CHEN Q Q, GUNDLACH M, YANG S Y, et al. Quantitative investigation of the mechanisms of microplastics and nanoplastics toward zebrafish larvae locomotor activity[J]. Sci Total Environ, 2017, 584-585: 1022-1031. DOI: 10.1016/j.scitotenv.2017.01.156.
[39] JEONG C B, WON E J, KANG H M, et al. Microplastic size-dependent toxicity, oxidative stress induction, and p-JNK and p-p38 activation in the monogonont rotifer(Brachionus koreanus)[J]. Environ Sci Technol, 2016, 50(16): 8849-8857. DOI: 10.1021/acs.est.6b01441.
[40] ANBUMANI S, KAKKAR P. Ecotoxicological effects of microplastics on biota: a review[J]. Environ Sci Pollut Res Int, 2018, 25(15): 14373-14396. DOI: 10.1007/s11356-018-1999-x.
[41] YANG W F, GAO P, MA G Y, et al. Transcriptome analysis of the toxic mechanism of nanoplastics on growth, photosynthesis and oxidative stress of microalga Chlorella pyrenoidosa during chronic exposure[J]. Environ Pollut, 2021, 284: 117413. DOI: 10.1016/j.envpol.2021.117413.
[42] SUN J, ZHANG Y, TAO S. Transcriptional response of zebrafish to polystyrene microplastics exposure implicate oxidative stress, immune dysfunction and metabolism disruption[J]. Environ Sci Technol, 2019, 53(10):6089-6099.
[43] ROCHMAN C M, KUROBE T, FLORES I, et al. Early warning signs of endocrine disruption in adult fish from the ingestion of polyethylene with and without sorbed chemical pollutants from the marine environment[J]. Sci Total Environ, 2014, 493: 656-661. DOI: 10.1016/j.scitotenv.2014.06.051.
[44] LEMOINE C M R, KELLEHER B M, LAGARDE R, et al. Transcriptional effects of polyethylene microplastics ingestion in developing zebrafish(Danio rerio)[J]. Environ Pollut, 2018, 243: 591-600. DOI: 10.1016/j.envpol.2018.08.084.
[45] ROMANO N, RENUKDAS N, FISCHER H, et al. Differential modulation of oxidative stress, antioxidant defense, histomorphology, ion-regulation and growth marker gene expression in goldfish(Carassius auratus)following exposure to different dose of virgin microplastics[J]. Comp Biochem Physiol C, 2020, 238: 108862. DOI: 10.1016/j.cbpc.2020.108862.
[46] LU Y F, ZHANG Y, DENG Y F, et al. Response to comment on “uptake and accumulation of polystyrene microplastics in zebrafish(Danio rerio)and toxic effects in liver”[J]. Environ Sci Technol, 2016, 50(22): 12523-12524. DOI: 10.1021/acs.est.6b04379.
[47] ZHANG X L, XIA M L, ZHAO J Y, et al. Photoaging enhanced the adverse effects of polyamide microplastics on the growth, intestinal health, and lipid absorption in developing zebrafish[J]. Environ Int, 2022, 158: 106922. DOI: 10.1016/j.envint.2021.106922.
[48] WU C, ZHAO A, ZHAO J, et al. Interactive toxic effects of microplastic and polychlorinated biphenyls on zebrafish(Danio rerio)embryos[J]. Chemosphere, 2021, 263:128327.
[49] 何君仪.微塑料与磷酸三苯酯对斑马鱼的联合毒性效应研究[D].南京:南京理工大学, 2019.
[50] 谢慧风.微塑料与重金属联合作用对红树白骨壤根际微生态及幼苗生长发育的影响[D].湛江: 广东海洋大学, 2022.
[51] SHARMA S, SINGH K, DHAR P, et al. Enhanced infectivity of Influenza A virus under the influence of microplastics[J]. J Hazard Mater, 2021, 403:123897.
[52] DA COSTA ARAU'JO A P, MALAFAIA G. Microplastic ingestion induces behavioral disorders in mice: a preliminary study on the trophic transfer effects via tadpoles and fish[J]. J Hazard Mater, 2021, 401: 123263. DOI: 10.1016/j.jhazmat.123263.
[53] ZHANG J J, WANG L, TRASANDE L, et al. Occurrence of polyethylene terephthalate and polycarbonate microplastics in infant and adult feces[J]. Environ Sci Technol Lett, 2021, 8(11): 989-994. DOI: 10.1021/acs.estlett.1c00559.
[54] AMATO-LOURENÇO L F, CARVALHO-OLIVEIRA R, JU'NIOR G R, et al. Presence of airborne microplastics in human lung tissue[J]. J Hazard Mater, 2021, 416: 126124. DOI: 10.1016/j.jhazmat.2021.126124.
[55] WU D, FENG Y D, WANG R, et al. Pigment microparticles and microplastics found in human thrombi based on Raman spectral evidence[J]. J Adv Res, 2023, 49: 141-150. DOI: 10.1016/j.jare.2022.09.004.
[56] RAGUSA A, MATTA M, CRISTIANO L, et al. Deeply in plasticenta: presence of microplastics in the intracellular compartment of human placentas[J]. Int J Environ Res Public Health, 2022, 19(18): 11593. DOI: 10.3390/ijerph191811593.
[57] PRATA J C, DA COSTA J P, LOPES I, et al. Environmental exposure to microplastics: an overview on possible human health effects[J]. Sci Total Environ, 2020, 702: 134455. DOI: 10.1016/j.scitotenv.2019.134455.
[58] CHOI D, BANG J, KIM T, et al. In vitro chemical and physical toxicities of polystyrene microfragments in human-derived cells[J]. J Hazard Mater, 2020, 400: 123308. DOI: 10.1016/j.jhazmat.2020.123308. (
Options
文章导航

/

〈 〉

版权所有 © 《河北大学学报(自然科学版)》编辑部
地址：河北省保定市五四东路180号 河北大学期刊社　邮编：071002
本系统由北京玛格泰克科技发展有限公司设计开发　技术支持：support@magtech.com.cn