为研究黄芩苷对单增李斯特菌(Listeria monocytogenes, LM)活化的小鼠巨噬细胞炎性小体的影响,用四唑盐(methylthiazolyldiphenyl-tetrazolium bromide,MTT)法确定黄芩苷最适质量浓度梯度,选用25、50、100 μg/mL的黄芩苷分别与LM处理巨噬细胞,检测细胞炎性小体相关蛋白或mRNA表达水平以及乳酸脱氢酶(lactate dehydrogenase, LDH)释放量.结果显示:黄芩苷剂量依赖性地下调了LM处理后的IL-1β、caspase-1 p10蛋白水平,LDH的释放量以及NLRP3、NLRC4、NLRP10、NOD、AIM2、caspase-1、IL-1β、IL-18的mRNA水平.因此推测,黄芩苷可以通过抑制LM诱导巨噬细胞内不同炎性小体的激活,进而抑制巨噬细胞炎症因子的释放及细胞死亡.
In order to study the effect of baicalin on the inflammasome of mouse macrophages activated by Listeria monocytogenes(LM), the optimal concentration gradient of baicalin was determined by methylthiazolyldiphenyl-tetrazolium bromide(MTT)method. Then macrophages were treated with LM and baicalin at concentrations of 25, 50 and 100 μg/mL, respectively, to detect the expression level of inflammasome-related protein or mRNA and lactate dehydrogenase(LDH)release. The results showed that baicalin reduced IL-1β, caspase-1 p10 protein levels, LDH release and mRNA levels of NLRP3, NLRC4, NLRP10, NOD, AIM2, caspase-1, IL-1β and IL-18 after LM treatment in a dose-dependent manner. Therefore, it is speculated that baicalin can inhibit the release of inflammatory factors and cell death in macrophages by inhibiting the activation of different inflammasome in macrophages induced by LM.
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