为探讨鳖甲煎丸(biejiajian pill,BJJP)对胰腺癌细胞增殖、凋亡和上皮-间充质转化(EMT)的干预作用.采用CCK-8、集落形成、Western blot、TUNEL划痕、Transwell和免疫荧光等实验方法检测BJJP含药血清对人正常胰腺导管上皮细胞HPNE活力的影响,对胰腺癌细胞PANC-1增殖、凋亡、迁移、侵袭、EMT以及PI3K/AKT通路蛋白表达的影响.结果显示:BJJP含药血清对HPNE细胞的活力无明显影响,具有抑制PANC-1细胞的增殖、迁移、侵袭、EMT进程的能力,促进了PANC-1细胞凋亡,抑制了PI3K/AKT通路,综上,鳖甲煎丸能抑制胰腺癌PANC-1细胞的恶性生物学行为,其机制可能与调节PI3K/AKT通路有关.
To investigate the effects of BJJP on the proliferation, apoptosis, and EMT of PANC-1 pancreatic cancer cells.CCK-8, Colony formation, Western blot, TUNEL scratch, Transwell and immunofluorescence assays were used to detect the effect of BJJP-containing serum on the activity of human normal pancreatic ductal epithelial cells HPNE, and detect the effects of BJJP-containing serum on the proliferation, apoptosis, migration, invasion, EMT, and the protein expression of PI3K/AKT pathway of pancreatic cancer cells PANC-1. The results showed that BJJP-containing serum has no significant effect on the activity of HPNE cells but inhibits the proliferation, migration, invasion, EMT- DOI:10.3969/j.issn.1000-1565.2025.01.008鳖甲煎丸对胰腺癌细胞PANC-1增殖、凋亡和EMT的干预作用熊亚南1,李以恒1,张荣花1,章广玲2,刘志勇2(1.华北理工大学 基础医学院, 河北省慢性疾病重点实验室, 河北 唐山 063210;2.华北理工大学附属医院,河北省医工融合精准医疗重点实验室, 河北 唐山 063000)摘 要:为探讨鳖甲煎丸(biejiajian pill,BJJP)对胰腺癌细胞增殖、凋亡和上皮-间充质转化(EMT)的干预作用.采用CCK-8、集落形成、Western blot、TUNEL划痕、Transwell和免疫荧光等实验方法检测BJJP含药血清对人正常胰腺导管上皮细胞HPNE活力的影响,对胰腺癌细胞PANC-1增殖、凋亡、迁移、侵袭、EMT以及PI3K/AKT通路蛋白表达的影响.结果显示:BJJP含药血清对HPNE细胞的活力无明显影响,具有抑制PANC-1细胞的增殖、迁移、侵袭、EMT进程的能力,促进了PANC-1细胞凋亡,抑制了PI3K/AKT通路,综上,鳖甲煎丸能抑制胰腺癌PANC-1细胞的恶性生物学行为,其机制可能与调节PI3K/AKT通路有关.关键词:鳖甲煎丸;胰腺癌;增殖;凋亡;上皮-间充质转化中图分类号:R735.9 文献标志码:A 文章编号:1000-1565(2025)01-0070-12Intervention effects of BJJP on the proliferation, apoptosis, and EMT of PANC-1 pancreatic cancer cellsXIONG Yanan1,LI Yiheng1,ZHANG Ronghua1,ZHANG Guangling2,LIU Zhiyong2(1. Hebei Key Laboratory for Chronic Diseases, School of Basic Medicine, North China University of Science and Technology, Tangshan 063210, China; 2. Hebei Provincial Key Laboratory of Medical-Industrial Integration Precision Medicine, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, China)Abstract: To investigate the effects of BJJP on the proliferation, apoptosis, and EMT of PANC-1 pancreatic cancer cells.CCK-8, Colony formation, Western blot, TUNEL scratch, Transwell and immunofluorescence assays were used to detect the effect of BJJP-containing serum on the activity of human normal pancreatic ductal epithelial cells HPNE, and detect the effects of BJJP-containing serum on the proliferation, apoptosis, migration, invasion, EMT, and the protein expression of PI3K/AKT pathway of pancreatic cancer cells PANC-1. The results showed that BJJP-containing serum has no significant effect on the activity of HPNE cells but inhibits the proliferation, migration, invasion, EMT- 收稿日期:2024-06-12;修回日期:2024-09-14 基金项目:河北省自然科学基金资助项目(H2023209047;H202409077);河北省教育厅在读研究生创新能力培养项目(CXZZSS2024056) 第一作者:熊亚南(1978—),女,华北理工大学副教授,主要从事胰腺癌分子机制研究.E-mail:xiongyanan@ncst.edu.cn 通信作者:刘志勇(1971—),男,华北理工大学教授,博士,主要从事胰腺癌分子机制研究.E-mail:macromicro@126.com 第1期熊亚南等:鳖甲煎丸对胰腺癌细胞PANC-1增殖、凋亡和EMT的干预作用河北大学学报(自然科学版) 第45卷process and PI3K/AKT pathway, enhances the apoptosis of pancreatic cancer PANC-1 cells.Studies have shown that BJJP inhibits the malignant biological behavior of pancreatic cancer PANC-1 cells, and the mechanism may be related to the regulation of PI3K/AKT pathway.
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