为检测有机磷农药在环境及食品中的残留,建立了一种基于胶体金的比色免疫层析检测方法.在最优实验条件下,建立了标准曲线.该检测方法对杀螟硫磷、甲基对硫磷和对硫磷3种有机磷农药的检出限分别可达0.94、0.32、0.03 ng/mL,适用于有机磷农药残留的初步筛选.对桃子样品进行加标回收,结果显示:杀螟硫磷的回收率为88%~113%,相对标准偏差≤10%;甲基对硫磷的回收率为107%~118%,相对标准偏差≤5%;对硫磷的回收率为106%~113%,相对标准偏差≤5%.实验结果表明,本方法具有较高的准确性和可靠性,且不易受样品基质的影响,能够准确检测桃汁中的有机磷农药残留.
In this study, a colorimetric immunochromatography method based on colloidal gold was developed. Under optimal experimental conditions, the standard curve was established. The detection limits of this method for the three organophosphorus pesticides were 0.94, 0.32, 0.03 ng/mL, respectively, and the method was suitable for the preliminary screening of organophosphorus pesticide residues.The recovery of peach samples by standard addition showed that the recoveries rate of fenitrothion were 88%—113%, and the relative standard deviation were less than 10%. The recoveries rate of parathion methyl ranged were 107%—118%, and the relative standard deviation were less than 5%. The recoveries rate of parathion were 106%—113%, and the relative standard deviation were less than 5%.The experimental results showed that the method had high accuracy and reliability, and was not easily affected by the sample matrix, and could accurately detect organophosphorus pesticide residues in peach juice.
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