To investigate changes in the gene expression profile and identify key inflammatory genes and signaling pathways involved in baicalin(BA)intervention against Listeria monocytogenes(LM)infection in murine macrophages, cells were divided into three groups:Control, LM-infected(LM), and BA-treated plus LM-infected(LM+BA). Following treatment, transcriptome sequencing(RNA-seq)was performed. Differentially expressed genes(DEGs)were screened and subjected to protein-protein interaction(PPI)analysis, Gene Ontology(GO)enrichment, and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis. Key findings were validated using reverse transcription-quantitative polymerase chain reaction(RT-qPCR). The results revealed 744 DEGs by comparing the LM group vs. the control group and the LM+BA group vs. the LM group. PPI analysis identified 82 core DEGs. These DEGs were primarily enriched in six inflammatory signaling pathways. RT-qPCR validation confirmed that the mRNA expression trends were consistent with the RNA-seq data. Therefore, we established BAs regulatory targets within LM-triggered inflammatory pathways, advancing our understanding of its anti-inflammatory properties and proposing new avenues for listeriosis control.- 引用格式:冯忠居,于明威,张聪,等.冲刷场地地震波形对大直径变截面单桩时程响应的影响［J］.河北大学学报(自然科学版),2026,46(4):337-348.引用格式:张恩华,张宗昊,陈静,等.黄芩苷对LM感染的巨噬细胞炎性通路的影响［J］.河北大学学报(自然科学版),2026,46(4):383-393.DOI:10.3969/j.issn.1000-1565.2026.04.006黄芩苷对LM感染的巨噬细胞炎性通路的影响张恩华1,2,张宗昊1,陈静1,赵子昂1,魏熙1,高政1,李文艳1(1.河北大学 基础医学院,河北 保定 071000;2. 河北大学 生命科学学院,河北 保定 071002)摘 要:为探究黄芩苷(baicalin,BA)干预单核细胞增生李斯特菌(Listeria monocytogenes, LM)感染小鼠巨噬细胞后基因表达谱的变化及其涉及的炎症关键基因和信号通路,将巨噬细胞分为3组:对照组、LM组和LM+BA组.对细胞处理后进行转录组测序,筛选出差异表达基因进行蛋白质互作、GO和KEGG分析,并通过RT-qPCR验证.结果显示:交叉分析LM组和对照组、LM+BA组和LM组,获得744个差异表达基因.蛋白质互作分析获得82个核心差异基因,主要富集的炎性通路6条.RT-qPCR结果表明mRNA表达趋势与测序结果相一致.因此,本研究筛选出了BA可能干预的LM引起炎症的相关信号通路和差异表达基因,为BA的抗炎机制和李斯特菌病的防治提供新思路.关键词:单核细胞增生李斯特菌;黄芩苷;转录组;RAW264.7细胞;炎性通路中图分类号:R392.12 文献标志码:A 文章编号:1000-1565(2026)04-0383-11DOI:10.3969/j.issn.1000-1565.2026.04.006Effect of baicalin on the inflammatory pathway of macrophages in LM infectionZHANG Enhua1,2, ZHANG Zonghao1, CHEN Jing1, ZHAO Ziang1, WEI Xi1, GAO Zheng1, LI Wenyan1(1. Basic Medical College, Hebei University, Baoding 071000,China;2. College of Life Sciences, Hebei University, Baoding 071002, China)Abstract: To investigate changes in the gene expression profile and identify key inflammatory genes and signaling pathways involved in baicalin(BA)intervention against Listeria monocytogenes(LM)infection in murine macrophages, cells were divided into three groups:Control, LM-infected(LM), and BA-treated plus LM-infected(LM+BA). Following treatment, transcriptome sequencing(RNA-seq)was performed. Differentially expressed genes(DEGs)were screened and subjected to protein-protein interaction(PPI)analysis, Gene Ontology(GO)enrichment, and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis. Key findings were validated using reverse transcription-quantitative polymerase chain reaction(RT-qPCR). The results revealed 744 DEGs by comparing the LM group vs. the control group and the LM+BA group vs. the LM group. PPI analysis identified 82 core DEGs. These DEGs were primarily enriched in six inflammatory signaling pathways. RT-qPCR validation confirmed that the mRNA expression trends were consistent with the RNA-seq data. Therefore, we established BAs regulatory targets within LM-triggered inflammatory pathways, advancing our understanding of its anti-inflammatory properties and proposing new avenues for listeriosis control.- 收稿日期:2025-06-26;修回日期:2025-09-27 基金项目:河北大学创新训练计划项目(S202510075134;DC2026560);河北大学实验室开放项目(sy202567);河北省中医药管理局项目(2023136) 第一作者:张恩华(1998—),女,河北大学在读博士研究生,主要从事分子生物学研究.E-mail:ly2580233@163.com 通信作者:李文艳(1973—),女,河北大学副教授,博士,主要从事生殖生物学研究.E-mail:liwenyan358@163.com 第4期张恩华等:黄芩苷对LM感染的巨噬细胞炎性通路的影响河北大学学报(自然科学版) 第46卷
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