PPM1A inhibits the migration, invasion and EMT process of PANC-1 pancreatic cancer cells

doi:10.3969/j.issn.1000-1565.2024.02.007

Abstract

Abstract: To investigate the effects of protein phosphatase magnesium-dependent 1A(PPM1A)on the migration, invasion and epithelial-mesenchymal transformation(EMT)in pancreatic cancer(PC), bioinformatics analysis and cellular immunofluorescence methods were used to study the expression of PPM1A. Wound healing, Transwell, qRT-PCR, Western blot and immunofluorescence tests were used to detect the effects of PPM1A on the migration and invasion of PANC-1 cells and the expression level of EMT- DOI:10.3969/j.issn.1000-1565.2024.02.007PPM1A抑制胰腺癌细胞PANC-1的迁移侵袭和EMT进程黄金平¹,张荣花¹,王梅梅¹,熊亚南¹,章广玲²(1. 华北理工大学基础医学院,河北省慢性疾病重点实验室,河北唐山 063210;2. 华北理工大学临床医学院,河北省医工融合精准医疗重点实验室,河北唐山 063210)摘要:为研究镁依赖性蛋白磷酸酶 1A(protein phosphatase magnesium-dependent 1A,PPM1A)对胰腺癌细胞迁移、侵袭及上皮-间充质转化(epithelial-mesenchymal transformation,EMT)的影响,采用生物信息学技术以及细胞免疫荧光实验分析PPM1A在胰腺癌中的表达情况,划痕、Transwell、qRT-PCR、Western blot、细胞免疫荧光实验检测PPM1A对胰腺癌细胞PANC-1迁移、侵袭及EMT标志物表达水平的影响,生物信息学网站预测PPM1A的上游miRNA.结果表明,PPM1A在胰腺癌中低表达,抑制PANC-1细胞的迁移、侵袭和EMT进程,miR-105-5p为PPM1A的潜在上游miRNA.说明PPM1A和miR-105-5p可能是治疗胰腺癌新的靶点.关键词:PPM1A;上皮-间充质转化;胰腺癌;迁移;侵袭中图分类号:R735.9 文献标志码:A 文章编号:1000-1565(2024)02-0164-09PPM1A inhibits the migration, invasion and EMT process of PANC-1 pancreatic cancer cellsHUANG Jinping¹, ZHANG Ronghua¹, WANG Meimei¹, XIONG Yanan¹, ZHANG Guangling²(1. Hebei Key Laboratory for Chronic Diseases, School of Basic Medicine, North China University of Science and Technology, Tangshan 063210, China; 2. Hebei Provincial Key Laboratory of Medical-Industrial Integration Precision Medicine, School of Clinical Medicine, North China University of Science and Technology, Tangshan 063210, China)Abstract: To investigate the effects of protein phosphatase magnesium-dependent 1A(PPM1A)on the migration, invasion and epithelial-mesenchymal transformation(EMT)in pancreatic cancer(PC), bioinformatics analysis and cellular immunofluorescence methods were used to study the expression of PPM1A. Wound healing, Transwell, qRT-PCR, Western blot and immunofluorescence tests were used to detect the effects of PPM1A on the migration and invasion of PANC-1 cells and the expression level of EMT- 收稿日期:2023-08-17;修回日期:2023-11-07 基金项目:河北省自然科学基金资助项目(H2021209026;H2023209047);河北省人力资源和社会保障厅项目(C20210340);河北省重点研发计划项目(213777115D);河北省财政厅项目(冀财预复［2020］397号) 第一作者:黄金平(1998—),女,华北理工大学在读硕士研究生,主要从事胰腺癌分子机制研究.E-mail:2185544026@qq.com 通信作者:熊亚南(1978—),女,华北理工大学副教授,主要从事胰腺癌分子机制研究.E-mail:xiongyanan@ncst.edu.cn章广玲(1972—),女,华北理工大学教授,博士,主要从事胰腺癌分子机制研究.E-mail:zhanggl@ncst.edu.cn第2期黄金平等:PPM1A抑制胰腺癌细胞PANC-1的迁移侵袭和EMT进程markers. The bioinformatics website predicts upstream miRNAs of PPM1A.The results showed that PPM1A was underexpressed in PC and inhibited the migration, invasion and EMT process of PANC-1 cells, miR-105-5p was a potential upstream miRNA of PPM1A, and PPM1A and miR-105-5p may be new targets for the treatment of pancreatic cancer.

Key words: PPM1A, epithelial-mesenchymal transformation, pancreatic cancer, migration, invasion

CLC Number:

R735.9

HUANG Jinping, ZHANG Ronghua, WANG Meimei, XIONG Yanan, ZHANG Guangling. PPM1A inhibits the migration, invasion and EMT process of PANC-1 pancreatic cancer cells[J]. Journal of Hebei University(Natural Science Edition), 2024, 44(2): 164-172.

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