Telomerase elongated product has many repeat sequences-(ggttag)n.A specific DNA probe-(ctaacc)2 which is matched with two repeat sequences is used to hybridize with telomerase elongated product.Excess DNA probes are attached to magnetic graphene and removed by magnetic separation.The DNA probes hybridized with telomerase elongated product is rapidly amplified by isothermal exponential amplification reaction(IEXPAR).SYBR Green I is utilized for real-time fluorescent detection of IEXPAR products.Under the optimal conditions,the telomerase activity in only 50 cancer cells can be easily detected.Sensitive detection of telomerase activity under isothermal condition is achieved.
ZHOU Xiaoyu
,
ZHANG Jiayu
,
ZHOU Man
,
JIA Hongxia
. Sensitive detection of telomerase activity based on isothermal exponetial amplification reaction[J]. Journal of Hebei University(Natural Science Edition), 2016
, 36(2)
: 155
-161
.
DOI: 10.3969/j.issn.1000-1565.2016.02.008
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