A new electropherogram mode was proposed by transforming time x-scale into minus reciprocal of the product of migration time and applied voltage. After a proper peak in the new electropherogram was assigned as the reference, each electropherogram was then moved along x-scale at a proper distance. The positive effect of new electropherogram on visual comparison was demonstrated by analyzing Radix paeoniae rubrain in capillary zone electrophoresis and mecellar electrokinetic chromatography. The relative standard deviation (RSD) in migration time was around 33% for each chosen peak when applied voltage varied from 15 kV to 30 kV, while the RSDs were dropped into the range from 0.17% to 0.70%. All results indicated that the proposed transformation method can significantly improve the visualization comparison of the electropherograms obtained at different voltages, in different sample matrices.
ZHANG Hong-yi
,
WANG Yan-xia
,
JING Cong
,
CHEN Hui
. Improvement of Visualization Comparison for Traditional Chinese Medicine Electropherogram by Transformation Time x-scale[J]. Journal of Hebei University(Natural Science Edition), 2010
, 30(2)
: 152
-157
.
DOI: 10.3969/j.issn.1000-1565.2010.02.010
[1] 何攀, 孙汉文, HE Pan, SUN Han-wen. 毛细管电泳-前沿分析法在药物-蛋白结合研究中的应用 [J]. 河北大学学报（自然科学版） 2009.doi:10.3969/j.issn.1000-1565.2009.02.024
[2] 颜磊, 龚萍, 袁林, 蒋雪梅, 夏之宁, YAN Lei, GONG Ping, YUAN Lin, JIANG Xuemei, XIA Zhining. 具有油水分配系数测算功能的中药指纹图谱 [J]. 色谱 2007.doi:10.3321/j.issn:1000-8713.2007.03.010
[3] 孙毓庆, 孙国祥, 金郁, SUN Yuqing, SUN Guoxiang, JIN Yu. 毛细管电泳指纹图谱及毛细管电泳-质谱联用在中药质量控制中的作用 [J]. 色谱 2008.doi:10.3321/j.issn:1000-8713.2008.02.006
[4] 韩晔华, 霍飞凤, 杨悠悠, 廖一平, 刘虎威, HAN Yehua, HUO Feifeng, YANG Youyou, LIAO Yiping, LIU Huwei. 中药指纹图谱研究的某些进展与展望 [J]. 色谱 2008.doi:10.3321/j.issn:1000-8713.2008.02.004
[5] 张红医, 李前锋, 刘惠涛, 陈兴国, 胡之德, 王锐. 一种新型毛细管区带电泳谱图记录模式的研究 [J]. 高等学校化学学报 2000.doi:10.3321/j.issn:0251-0790.2000.12.009
[6] DRA(s)AR P, MORAVCOVA J. Recent advances in analysis of Chinese medical plants and traditional medicines [J]. Journal of Chromatography B 2004, 812(1/2).
[7] Gomez JE, Sandoval E. The effect of conditioning of fused-silica capillaries on their electrophoretic performance [J]. Electrophoresis: The Official Journal of the International Electrophoresis Society 2008, 2(2).
[8] Gordon GA. VOLTAGE PRE-CONDITIONING TECHNIQUE FOR OPTIMISATION OF MIGRATION-TIME REPRODUCIBILITY IN CAPILLARY ELECTROPHORESIS [J]. Journal of chromatography, A: Including electrophoresis and other separation methods 1995, 2(2).
[9] LEETT, YEUNG ES. Facilitating data transfer and improving precision in capillary zone electrophoresis with migration indexes [J]. Analytical Chemistry 1991, 63(24).
[10] Yang J, Rose S, Hage DS. IMPROVED REPRODUCIBILITY IN CAPILLARY ELECTROPHORESIS THROUGH THE USE OF MOBILITY AND MIGRATION TIME RATIOS [J]. Journal of chromatography, A: Including electrophoresis and other separation methods 1996, 1/2(1/2).
[11] Mathai Mammen, lan J. Colton, Jeffrey D. Carbeck. Representing Primary Electrophoretic Data in the 1/Time Domain: Comparison to Representations in the Time Domain [J]. Analytical chemistry 1997, 11(11).
[12] YING Meiliu, SHUENN J. Determination of the six major flavonoids in Scuttellariae Radix by micellar electrokinetic capillary electrophoresis [J]. Analytica Chimica Acta 1994, 288.
[13] 吕建德, 傅晓芸. 样品溶液基质对毛细管电泳分离的影响 [J]. 分析化学 1994, 22(12).
