The modification conditions were optimized for the preparation of sulfated lycium barbarum polysaccharide (SLBP) by using chlorsulfonic acid-pyridine method by L_9(3)~4 orthogonal design. The optimized conditions were 1∶4 of chlorosulfonic acid-topyridine, reaction temperature of 80 ℃ and reaction time of 2 h. The content of sulfur in SLBP and the degree of substitution (DS) were achieved to be 17.23% and 1.935, respectively. The nature of SLBP was characterized by UV spectra and infrared spectra analysis, and the inhibiting effect of SLBP to growth of Hela cell in vitro was tested with MTT method. In the range of 25～200 mg/L, LBP had little inhibited activity towards the Hela cells, while SLBP inhibited the growth of Hela cells remarkably with 31.71% inhibitory ratio. The SLBP prepared by the proposed sulfated method has higher sulfur content and degree of substitution, and obviously inhibiting effect to growth of Hela cells. The inhibitory ratio is positive correlation with the substitution degree and the tested dosage.
SUN Han-wen
,
XU Cheng-yan
,
ZHAO Yan-yan
,
LIANG Shu-xuan
. Sulfation for Modification of Lycium Barbarum Polysaccharide and Its Inhibiting Effect to Growth of Hela Cells in Vitro[J]. Journal of Hebei University(Natural Science Edition), 2009
, 29(6)
: 591
-595
.
DOI: 10.3969/j.issn.1000-1565.2009.06.009
[1] 刘占峰, 孙汉文, LIU Zhan-feng, SUN Han-wen. 多糖的化学修饰研究进展 [J]. 河北大学学报（自然科学版） 2005.doi:10.3969/j.issn.1000-1565.2005.01.024
[2] 黄小燕, 孔祥峰, 王德云, 胡元亮, HUANG Xiao-yan, KONG Xiang-feng, WANG De-yun, HU Yuan-liang. 多糖硫酸化修饰和多糖硫酸酯的研究进展 [J]. 天然产物研究与开发 2007.doi:10.3969/j.issn.1001-6880.2007.02.039
[3] 栗衍华, 谭成玉, 王秀武, 白雪芳, 杜昱光, LI Yan-hua, TAN Cheng-yu, WANG Xiu-wu, BAI Xue-fang, DU Yu-guang. 硫酸化多糖的制备及其生物活性的研究进展 [J]. 精细与专用化学品 2006.doi:10.3969/j.issn.1008-1100.2006.16.002
[4] 邹明畅, 崔飞伦, 倪鸿昌, 蔡宝昌. 多糖硫酸酯抗肿瘤作用的研究进展 [J]. 现代中药研究与实践 2008.doi:10.3969/j.issn.1673-6427.2008.03.019
[5] 彭宗根, 陈鸿珊, 郭志敏, 董飚, 田庚元, 王光强, PENG Zong-gen, CHEN Hong-shan, GUO Zhi-min, DONG Biao, TIAN Geng-yuan, WANG Guang-qiang. 牛膝多糖硫酸酯体外和体内抗艾滋病病毒作用 [J]. 药学学报 2008.doi:10.3321/j.issn:0513-4870.2008.07.006
[6] 易剑平, 闫红, 钟儒刚, YI Jian-ping, YAN Hong, ZHONG Ru-gang. 微波法硫酸酯化枸杞多糖的研究 [J]. 食品科技 2006.doi:10.3969/j.issn.1005-9989.2006.04.009
[7] 张晶, 韩喜江, Zhang-jing, Han xi-jiang. 东北枸杞多糖提取分离工艺优化 [J]. 黑龙江大学自然科学学报 2007.doi:10.3969/j.issn.1001-7011.2007.06.016
[8] 刘燕琼, 黄雪松, LIU Yan-qiong, HUANG Xue-song. 硫酸化大蒜多糖的制备及其鉴定 [J]. 食品科学 2007.doi:10.3321/j.issn:1002-6630.2007.02.020
[9] 乐晓桐, 尹鸿萍, 王旻, 徐士君, LE Xiao-tong, YIN Hong-ping, WANG Min, XU Shi-jun. 低分子螺旋藻多糖的制备及其硫酸酯化物体外抗肿瘤活性的初步研究 [J]. 药物生物技术 2006.doi:10.3969/j.issn.1005-8915.2006.02.010
[10] 何晋浙, 胡飞华, 孙培龙, 张安强, 邵平, He Jinzhe, Hu Feihua, Sun Pei Long, Zhang Anqiang, Shao Ping. 枸杞多糖结构及其单糖组分的分析研究 [J]. 食品与发酵工业 2008.
[11] 王战勇, 闫松, 商丽颖, 晁彩林, WANG Zhan-yong, YAN Song, SHANG Li-ying, CHAO Cai-lin. 枸杞多糖的提取纯化及组成分析 [J]. 氨基酸和生物资源 2008.doi:10.3969/j.issn.1006-8376.2008.01.006
[12] 冯秀梅, 陈邦银, 张汉萍. 黄芪多糖硫酸酯的合成及其抗病毒活性研究 [J]. 中国药科大学学报 2002.doi:10.3321/j.issn:1000-5048.2002.02.018
[13] 陈景耀, 吴国荣, 王建安, 陆长梅, 张卫明, 蒋继宏, CHEN Jing-yao, WU Guo-rong, WANG Jian-an, LU Chang-mei, ZHANG Wei-ming, JIANG Ji-hong. 正交试验优选白芨多糖硫酸酯化工艺的研究 [J]. 中草药 2005.doi:10.3321/j.issn:0253-2670.2005.01.019
[14] Preeyanat Vongchan, Warayuth Sajomsang, Dumrat Subyen, Prachya Kongtawelert. Anticoagulant activity of a sulfated chitosan [J]. Carbohydrate research 2002, 13(13).
[15] Koyanagi S, Tanigawa N, Nakagawa H, Soeda S, Shimeno H. Oversulfation of fucoidan enhances its anti-angiogenic and antitumor activities. [J]. Biochemical Pharmacology 2003, 2(2).
[16] 孙汉文, 刘占锋, SUN Han-wen, LIU Zhan-feng. 枸杞多糖的超声波辅助水提取与分级纯化 [J]. 食品工业科技 2009.
