The two yeaststrainsYP2 and YZ13 were isolated from Chinese-spirit fermenting grains and they could all grow well under the condition of pH 3.0.Strain YP2 was identified as belonging to Candida and the similarity degree of strain YP2 and Candida pseudolambica(KM384061.1)reached 98%,while strain YZ13 to Pichia and the similarity degree with Pichia kudriavzevii(LC014798.1)reached 100%.The two strains were mixed at the ratio of 1∶1 in weight and applied to the fermentation of waste fermenting grains.The process of solid state fermentation lasted for 60 hours under the condition of inoculation quantity 100 mL/kg,fresh fermenting grains 870 g/kg,wheat bran 100 g/kg,ammonia sulfate 15 g/kg,quicklime 15 g/kg,temperature 28 ℃.The results showed that the protein content of the dry sample of waste fermenting grains reached 172.30 g/kg and increased by 28.29% and it enriched feed protein of waste fermenting grains and provided foundation for waste fermenting grains and high efficiency feed.
LI Yanqin
,
CUI Haihao
,
LI Chunqing
,
SONG Ruixue
,
CHEN Yajun
. Separation and identification of acid proof yeast and its application in fermentation of waste fermenting grains[J]. Journal of Hebei University(Natural Science Edition), 2016
, 36(5)
: 517
-523
.
DOI: 10.3969/j.issn.1000-1565.2016.05.011
[1] 郭志,明红梅,沈才萍,等.白酒丢糟饲料化的发酵培养基优化研究[J].粮食与饲料工业,2013(11):35-38.DOI:10.7631/j.issn.1003-6202.2013.11.010. GUO Z,MING H M,SHEN C P,et al.Composing optimization of fermentation medium in the progress of distilled grains turning into feed [J].Cereel & Feed Industry,2013(11):35-38.DOI:10.7631/j.issn.1003-6202.2013.11.010.
[2] 李进,梁丽静,薛正楷.中国传统白酒酿造丢糟资源循环利用研究进展[J].酿酒科技,2015(4):88-91.DOI:10.13746/j.njkj.2014395. LI J,LIANG L J,XUE Z K.Research progress in the reclamation of waste spent grains from traditional liquor-making[J].Liquor-making Science & Technology,2015(4):88-91.DOI:10.13746/j.njkj.2014395.
[3] 王印召,吴正云,杨健,等.白酒丢糟资源化利用的研究进展[J].酿酒科技,2013(9):86-89.DOI:10.13746/j.njkj.2013.09.037 86. WANG Y Z,WU Z Y,YANG J,et al.Research progress in the reclamation of distiller's grains[J].Liquor-Making Science & Technology,2013(9):86-89.DOI:10.13746/j.njkj.2013.09.037 86.
[4] 陈娟,王治业,魏甲乾,等.多菌种分步固态发酵果渣生产菌体蛋白饲料的工艺优化[J].中国酿造,2014,33(3):40-44. DOI:10.3969/j.issn.0254-5071.2014.03.011. CHEN J,WANG Z Y,WEI J Q,et al.Technology optimization of multi-strains multi-step solid-state fermentation of fruit residue to produce cell protein feeds[J].China Brewing,2014,33(3):40-44.DOI:10.3969/j.issn.0254-5071.2014.03.011.
[5] 沈萍,陈向东.微生物学实验[M].4版.北京:高等教育出版社,2010:242.
[6] 张纪忠.微生物分类学[M].上海:复旦大学出版社,1990.
[7] 李日强,张峰,韩文辉,等.不同菌株固态发酵废白酒糟生产饲料蛋白的研究[J].重庆环境科学,2003,25(11):63-64. DOI:10.3969/j.issn.1674-2842.2003.11.025. LI R Q,ZHANG F,HAN W H,et al.Study on the solid-state fermentation of distillers’grains to produce feeding-proteinby using some fungal strains[J].Chongqing Environmental Sciences,2003,25(11):63-64.DOI:10.3969/j.issn.1674-2842.2003.11.025.
[8] 武金霞.生物化学实验教程[M].北京:科学出版社,2012:167-172.
[9] 明红梅,霍丹群,周健,等.丢糟混菌发酵生产蛋白饲料的研究[J].安徽农业科学,2010,38(20):10739-10742.DOI:10.3969/j.issn.0517-6611.2010.20.099. MING H M,HUO D Q,ZHOU J,et al.Production of protein feedstuff by multiple microbes fermentation of waste lees[J].Journal of Anhui Agricultural Sciences,2010,38(20):10739-10742.DOI:10.3969/j.issn.0517-6611.2010.20.099.
