In order to explore the functional microorganism, the intestinal culturable microflora in Cathaica fasciola were screened and cultured by several kinds of culture media, and the cellulase activity of the isolated bacteria and the characteristics of the crude cellulase that produced by strain S6 was studied.The results showed that the largest number of intestinal bacteria was up to 3.3×104 CFU/mL in LB medium.Congo red staining showed that all the 7 dominant bacteria had the ability to secrete cellulase.The strain S6 had highest CMCase and FPase values of 5.96 and 2.84 U/mL, respectively.The CMCase characterization revealed that, the optimum reaction temperature was 35 ℃, the cellulose kept its high activity from 55 ℃to 75 ℃.The crude enzyme still had 60% of the highest obvious activation function at pH 8.0.Mg2+、Na+、K+and Ca2+ had enhancing effect, and Zn2+、Cu2+ and Mn2+ had inhibitory action.To research the composition of the snail intestinal bacteria and separate high-yield cellulase strains is a new way for the cellulose biodegradation into renewable energy.
CHANG Yanping
,
TIAN Qifan
,
GUO Xin
,
LI Yanqin
,
YAN Leilei
,
LI Chunqing
. Composition of the intestinal bacteria and their cellulase characterization in Cathaica fasciola[J]. Journal of Hebei University(Natural Science Edition), 2017
, 37(4)
: 400
-404
.
DOI: 10.3969/j.issn.1000-1565.2017.04.011蜗牛肠道细菌及其纤维素酶性质
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