In order to provide theoretical support and bacterial resources for the treatment of heavy metal pollution in Baiyangdian Lake, the phylogenetic diversity of heavy metal-resistant bacteria in Baiyangdian was studied using isolation and culture method, and the relationship between the distribution of heavy metal-resistant bacteria and ecological factors was analyzed. 54 strains of anti-80 mg/L Pb2+ bacteria, 46 strains of anti-80 mg/L Cu2+ bacteria, and 39 strains of anti-100 mg/L Cr(Ⅵ)bacteria were isolated- DOI:10.3969/j.issn.1000-1565.2018.06.016白洋淀抗重金属细菌多样性及其与生态因子相关性陈思雨1, 王丽娜1, 杨惠琪1, 陈玉1, 张瑾1, 吕志堂1,2(1.河北大学 生命科学学院 河北省微生物多样性研究与应用实验室, 河北 保定 071002;2.药物化学与分子诊断教育部重点实验室, 河北 保定 071002)摘 要:为了给白洋淀重金属污染治理提供理论支持和菌种资源, 采用分离培养的方法调查了白洋淀抗重金属细菌的系统发育多样性,并分析了抗重金属细菌分布与生态因子之间的关系. 从各采样点的样品中分离筛选得到抗80 mg/L Pb2+细菌54株、抗80 mg/L Cu2+细菌46株, 抗100 mg/L Cr(Ⅵ)细菌39株. 综合考虑各菌株的抗性及16S rRNA基因序列差异,将139株抗重金属菌去除重复后合并为69株, 分属于肠杆菌属(Enterobacter)、丛毛单胞菌属(Comamonas)、戴尔福特菌属(Delftia)、短波单胞菌属(Brevundimonas)、寡养单胞菌属(Stenotrophomonas)、假单胞菌属(Pseudomonas)和赖氨酸芽孢杆菌属(Lysinibacillus)共7个属13个种. 进一步采用PCA分析的方法对抗重金属细菌与生态因子相关性进行研究, 发现在菌群组成和分布范围方面抗Pb2+和Cu2+菌大体相似, 且假单胞菌属和寡养单胞菌属2个属容限最高, 表明菌株对于重金属具有交叉抗性;抗100 mg/L Cr(Ⅵ)细菌中赖氨酸杆菌属为特有属, 且分布广泛;白洋淀水域唯一能够同抗Pb2+、Cu2+和Cr(Ⅵ)3种重金属的细菌是寡养单胞菌属.关键词:白洋淀;抗重金属细菌;系统发育多样性;生态因子中图分类号:Q938.1+1 文献标志码:A 文章编号:1000-1565(2018)06-0664-09Phylogenetic Diversity of heavy metal-resistant bacteria in Baiyangdian Lake and its relationship with ecological factors CHEN Siyu1, WANG Lina1, YANG Huiqi1, CHEN Yu1, ZHANG Jin1, LÜ Zhitang1,2(1. Key Laboratory of Microbial Diversity Research and Application of Hebei Province,College of Life Sciences, Hebei University, Baoding 071002, China;2. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China)Abstract: In order to provide theoretical support and bacterial resources for the treatment of heavy metal pollution in Baiyangdian Lake, the phylogenetic diversity of heavy metal-resistant bacteria in Baiyangdian was studied using isolation and culture method, and the relationship between the distribution of heavy metal-resistant bacteria and ecological factors was analyzed. 54 strains of anti-80 mg/L Pb2+ bacteria, 46 strains of anti-80 mg/L Cu2+ bacteria, and 39 strains of anti-100 mg/L Cr(Ⅵ)bacteria were isolated- 收稿日期:2018-05-20 基金项目:河北省生物学强势特色学科项目(1050-5030004);河北省生物工程重点学科项目(1050-5030023) 第一作者:陈思雨(1994—),女,河北安新人,河北大学在读硕士研究生. E-mail:331255943@qq.com 通信作者:吕志堂(1973—),男,河北曲周人,河北大学教授,博士,主要从事微生物资源与系统学研究. E-mail:lzt325@126.com第6期陈思雨等:白洋淀抗重金属细菌多样性及其与生态因子相关性and screened from the sampling sites. A total of 69 strains were combined after repeated strains were removed based on heavy metal-resistant characteristics sequence difference, which belonged to 13 species distributed in 7 genera Enterobacter, Comamonas, Delftia, Brevundimonas, Stenotrophomonas, Pseudomonas, and Lysinibacillus. Further PCA analysis was used to study the relationship between heavy metal resistant bacteria and ecological factors. In terms of group composition and distribution, the anti-Pb2+ and Cu2+ bacteria were generally similar, and Pseudomonas and Stenotrophomonas had the highest abundance, indicating that the strain had cross-resistance to heavy metals. Lysinibacillus is the unique 100 mg/L Cr(Ⅵ)resistant genus, and it is widely distributed. Stenotrophomonas is the only genus which can survive in three kinds of heavy metals Pb2+, Cu2+ and Cr(Ⅵ)within Baiyangdian Lake.
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