Journal of Hebei University(Natural Science Edition) >

2019 , Vol. 39 >Issue 3: 254 - 263

DOI: https://doi.org/10.3969/j.issn.1000-1565.2019.03.006

DNA binding and photocleavage properties of a dinuclear ruthenium complex

  • WANG Shuang ,
  • XU Xuexue ,
  • MI Yaxuan ,
  • ZHENG Zebao ,
  • ZHAO Xiaolong
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  • 1. College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; 2. College of Chemistry, Taishan University, Taian 271021, China

Received date: 2018-11-20

  Online published: 2019-05-25

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Abstract

A dinuclear ruthenium complex has been synthesized and characterized. The calf thymus DNA(ct-DNA)binding properties of the complex are investigated by means of DNA viscosity and optical spectroscopic techniques of UV-visible absorption and emission spectral titrations, DNA thermal denaturation and reverse, salt effect and agarose gel electrophoresis. The results suggest that the complex binds to DNA through partial-intercalation mode with a large DNA binding constant of 106 L/mol order of magnitude. The complex is also demonstrated to be an efficient cleaver of pBR 322 DNA under irradiation at 360 nm.

Key words： ruthenium complex; DNA; bind; electrophoresis

Cite this article

WANG Shuang , XU Xuexue , MI Yaxuan , ZHENG Zebao , ZHAO Xiaolong . DNA binding and photocleavage properties of a dinuclear ruthenium complex[J]. Journal of Hebei University(Natural Science Edition), 2019 , 39(3) : 254 -263 . DOI: 10.3969/j.issn.1000-1565.2019.03.006

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