Journal of Hebei University(Natural Science Edition) >

2019 , Vol. 39 >Issue 5: 522 - 528

DOI: https://doi.org/10.3969/j.issn.1000-1565.2019.05.013

Photodynamic antibacterial and antitumor activity of photosensitizer T1

  • ZHAO Zhanjuan ,
  • MA Yingying ,
  • LIU Yameng ,
  • YANG Yanxi ,
  • SHANG Yazhen ,
  • ZHAO Jianxi
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  • 1. College of Basic Medical Science, Hebei University, Baoding 071002, China; 2. College of Public Health, Hebei University, Baoding 071002, China; 3. Department of Radiology, Affiliated Hospital of Hebei University, Baoding 071002, China

Received date: 2019-03-04

  Online published: 2019-09-25

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Abstract

To investigate the photodynamic therapy(PDT)effect of the same photosensitivities on tumor cells and drug-resistant bacteria, colon cancer cells(CT-26)and methicillin-resistant Staphylococcus aureus(MRSA)selected in vitro. The phagocytosis of photosensitizer T1 by MRSA and CT-26 was detected by laser confocal microscopy. The light and dark response curves of T1 to CT-26 cells and MRSA were determined by MTT staining and colony counting. The results showed that both CT-26 cells and MRSA absorbed T1 rapidly in a time-dependent and concentration-dependent manner. T1-mediated PDT showed strong inhibition on CT-26 cells and MRSA, and CT-26 cells and MRSA showed strong fluorescence signal of T1 by confocal laser microscopy. The proliferation of MRSA and CT-26 cells were significantly inhibited through T1-mediated PDT, and PDT may become a new method for the treatment of tumors which caused by bacterial infection in the future, providing experimental data for the clinical application of PDT.- DOI:10.3969/j.issn.1000-1565.2019.05.013光敏剂T1的光动力抗菌与抗肿瘤活性赵占娟1,马莹莹2,刘亚萌1,杨焱惜1,商亚贞3,赵建喜3(1. 河北大学 医学院,河北 保定 071002;2.河北大学 公共卫生学院,河北 保定 071002;3.河北大学附属医院 放射科,河北 保定 071002)摘 要:为了探讨同种光敏剂对肿瘤细胞及耐药细菌的光动力(photodynamic therapy,PDT)治疗效果,体外选取了金黄色葡萄球耐药菌(methicillin-resistant Staphylococcus aureus, MRSA)及结肠癌细胞(CT-26),采用激光共聚焦显微镜检测MRSA及CT-26对半乳糖酞菁类光敏剂T1的吞噬,四甲基偶氮唑蓝染色法(MTT法)和菌落计数法检测T1对CT-26细胞和MRSA的光反应及暗反应浓度曲线;结果发现CT-26细胞及MRSA对T1吸收迅速且呈时间和浓度依赖性. T1介导的PDT对CT-26细胞和MRSA均具有较强的抑制,激光共聚焦显微镜也显示T1在CT-26细胞和MRSA内都有很强的荧光信号.T1介导的PDT能明显抑制MRSA及CT-26细胞的增殖,未来有可能成为一种治疗由细菌感染引起肿瘤的新方法,为PDT的临床应用提供实验数据.关键词:光敏剂;光动力疗法;细菌;肿瘤细胞中图分类号:R735 文献标志码:A 文章编号:1000-1565(2019)05-0522-07Photodynamic antibacterial and antitumor activity of photosensitizer T1ZHAO Zhanjuan1, MA Yingying2, LIU Yameng1, YANG Yanxi1, SHANG Yazhen3, ZHAO Jianxi3(1. College of Basic Medical Science, Hebei University, Baoding 071002, China;2. College of Public Health, Hebei University, Baoding 071002, China;3. Department of Radiology, Affiliated Hospital of Hebei University, Baoding 071002, China)Abstract: To investigate the photodynamic therapy(PDT)effect of the same photosensitivities on tumor cells and drug-resistant bacteria, colon cancer cells(CT-26)and methicillin-resistant Staphylococcus aureus(MRSA)selected in vitro. The phagocytosis of photosensitizer T1 by MRSA and CT-26 was detected by laser confocal microscopy. The light and dark response curves of T1 to CT-26 cells and MRSA were determined by MTT staining and colony counting. The results showed that both CT-26 cells and MRSA absorbed T1 rapidly in a time-dependent and concentration-dependent manner. T1-mediated PDT showed strong inhibition on CT-26 cells and MRSA, and CT-26 cells and MRSA showed strong fluorescence signal of T1 by confocal laser microscopy. The proliferation of MRSA and CT-26 cells were significantly inhibited through T1-mediated PDT, and PDT may become a new method for the treatment of tumors which caused by bacterial infection in the future, providing experimental data for the clinical application of PDT.- 收稿日期:2019-03-04 基金项目:河北省卫生厅资助项目(20170833);河北大学大学生创新训练计划项目(2018307;2019027);河北大学医学院2018年实验室开放项目 第一作者:赵占娟(1974—),女,河北博野人,河北大学副教授,博士,主要从事激光医学方向研究. E-mail:zhaozhanjuanv@163.com 通信作者:赵建喜(1969—),男,河北保定人,河北大学教授,主要从事影像诊断方向研究.E-mail:jianxizhaov@163.com第5期赵占娟等:光敏剂T1的光动力抗菌与抗肿瘤活性

Key words： photosensitizer; photodynamic therapy; bacteria; tumor cell

Cite this article

ZHAO Zhanjuan , MA Yingying , LIU Yameng , YANG Yanxi , SHANG Yazhen , ZHAO Jianxi . Photodynamic antibacterial and antitumor activity of photosensitizer T1[J]. Journal of Hebei University(Natural Science Edition), 2019 , 39(5) : 522 -528 . DOI: 10.3969/j.issn.1000-1565.2019.05.013

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