The chemical constituents of the standard extracts of the four medicinal materials in Yinzhihuang formula were analyzed by HPLC, and determined the content of chlorogenic acid, 1,3-dicaffeoylquinic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, geniposide, baicalin, wogonin, baicalein, wogonoside, oroxylin A, and neochlorogenic acid. The separation was carried out on a Diamonsil C18(250 mm×4.6 mm,5 μm)column, and gradient eluted with the mobile phase of acetonitrile(A)-0.1% formic acid aqueous solution(B)with 1.0 mL/min. The column temperature was set at 30 ℃. The main components in the four standard medicinal extracts of Yinzhihuang formula can be separated in the chromatographic conditions. Precision, repeatability, and stability were good, and the average recoveries were between 98.84%~102.22%. The developed method was simple and effective for the determination of the components in the four medicinal materials, and the result is accurate and reliable, it can provide a reference for the Yinzhihuang formulation of the quality standard of medicinal extracts and a basis for the analysis of the main chemical components.
ZHANG Yushan
,
SUN Yifei
,
LUO Li
,
LI Zuoming
,
ZHANG Shanzi
,
YAO Xincheng
. Analysis and determination of the main components in the four medicinal materials of Yinzhihuang formula by HPLC[J]. Journal of Hebei University(Natural Science Edition), 2020
, 40(5)
: 500
-509
.
DOI: 10.3969/j.issn.1000-1565.2020.05.008
[1] 王玉蛟,王立.中医防治新生儿黄疸的研究进展[J].中国医药科学,2016, 6(1): 26-30.
[2] 马印图,李莉华,李玉秋,等.茵栀黄口服液临床应用研究进展[J].解放军医药杂志,2014,26(6): 88-91.DOI:10.3969/j.issn.2095.140x.201406.024.
[3] 范建伟,李蔚群,李艳芳,等.茵栀黄制剂处方渊源、剂型沿革与质量控制的研究进展[J].中成药,2017,39(11): 2365-2368.DOI:10.3969/j.issn.1001-1528.2017.11.031.
[4] 国家药典委员会.《中华人民共和国药典》 一部[M].2015年版. 北京: 中国医药科技出版社, 2015: 1146-1157.
[5] 慕永平,刘平,王磊.茵陈蒿汤的发展及现代研究[J].中国实验方剂学杂志,2006,12(2): 67-71.DOI:10.13422/j.cnki.syfjx.2006.02.026.
[6] 窦志华,陈智娴,王冬梅,等.茵陈蒿汤醇提与水提部位利胆保肝药效研究[J].中国中医药信息杂志,2012,19(9): 40-42.DOI:10.3969/j.issn.1005-5304.2012.09.014
[7] 邱小玉,刘玉萍,刘烨,等.HPLC法同时测定茵栀黄口服液中10种有效成分[J].中草药,2019,50(15): 3648-3653.DOI:10.7501/j.issn.0253-2670.2019.15.020.
[8] 张楠,许苗苗,王舒懿,等.基于HPLC波长切换联合梯度洗脱技术的茵栀黄口服液中15种成分定量研究[J].中草药, 2019, 50(18):4338-4345.DOI:10.7501/j.issn.0253-2670.2019.18.012.
[9] 张玉姗,刘谢英,姚新成,等.高效液相色谱法同时测定茵栀黄颗粒中14个化学成分[J].药物分析杂志, 2019, 39(7): 1229-1238.DOI:10.16155/j.issn.0254-1793.2019.07.09.
[10] 陈永刚,马传学,黄成琼,等.高效液相色谱-波长切换法同时测定茵栀黄颗粒中的7种有效成分[J].中国医院药学杂志,2012,32(11): 894-896.DOI:10.13286/j.cnki.chinbosppharmacyj.2012.11.007.
[11] 张昀,张艳萍,何峰.HPLC法测定茵陈提取物中绿原酸的含量[J].贵州医药,2015,39(10): 918-919.
[12] 何峰,张昀,张艳萍.HPLC法测定栀子提取物中栀子苷的含量[J].贵州医药,2017,41(3):245-247.
[13] 丁芳林,张雯杰,陈波,等.HPLC法测定黄芩提取物中4 种主要活性成分的含量[J].湖南农业科学,2008(3):128-130.DOI:10.16498/j.cnki.hnnykx.2008.03.054.
[14] 陈有根,王志斌,戴俊东.3种经典工艺生产的金银花提取物中绿原酸类成分含量比较[J].中成药,2007, 29(3): 362-364.
[15] 范毅,陈玲,李晓,等.不同产地金银花提取物的指纹图谱及绿原酸、木犀草苷含量测定[J].河南科学,2019, 37(1):53-59.
