This work centered on the key role of soybean callose synthase in the process of soybean mosaic virus infection. Twenty four callose synthase were identified in soybean by bioinformatics in this experiment. Glyma.08G308200(GmGSL7c)was selected for RT-qPCR to verify that the transcriptome data was consistent with the actual expression trend.TRV-VIGS followed by callose fluorescence observation found that silencing GmGSL7c affected the synthesis of callose in the leaves, and the SMV-CP gene could be detected in the upper leaves of the silenced plants that were not inoculated. In addition, disease symptoms of SMV were observed in the GmGSL7c silenced plants, indicating that the silencing of GmGSL7c promotes the transportation of SMV in soybeans. This study lays the foundation for further research on the soybean-SMV interaction signal pathway for disease resistance.
WANG Mengxuan
,
SUN Xizhe
,
SUN Tianjie
,
ZHANG Jie
,
WANG Dongmei
. Effect of GmGSL7c in SMV infected soybean[J]. Journal of Hebei University(Natural Science Edition), 2021
, 41(1)
: 47
-54
.
DOI: 10.3969/j.issn.1000-1565.2021.01.007
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