Journal of Hebei University(Natural Science Edition) >

2021 , Vol. 41 >Issue 2: 195 - 200

DOI: https://doi.org/10.3969/j.issn.1000-1565.2021.02.013

Reproductive growth of engineered strains of Auricularia polytricha cells under Auricularia auricularia bran matrix

  • LIU Wei ,
  • XU Fan ,
  • YANG Chaoshang ,
  • GENG Danmeng ,
  • ZHANG Tiejun ,
  • WANG Qian
Expand
  • 1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. Agricultural Environmental Protection Monitoring Station, Bureau of Agriculture and Rural Affairs of Chengde City, Chengde 067000, China

Received date: 2020-03-28

  Online published: 2021-04-07

Fold

Abstract

The Auricularia polytricha(Mont.)Sacc cell engineering strain Xinheda SL205 was used as the test strain, the A. auricularia bran was used as the main reproductive growth substrate, and the mycelium growth rate and reproductive growth biological efficiency were used as indicators. Different medium formulations were designed and tested.The optimal medium formulations were selected for the Xinheda SL205 strain: A. auricularia bran is 50%(mass fraction), sawdust 30%, corn cob 10%, bran 8% and lime 2%. Under this medium, the average growth rate of Xinheda SL205 was 7.26 mm/d, and the biological efficiency was 126.29%. It had the strongest biodegradation ability for A. auricularia bran, effectively reducing the non-point source pollution caused by A. auricularia bran.

Key words： Auricularia polytricha; Auricularia auricularia bran; resource-based development; reproductive growth; bioconversion rate

Cite this article

LIU Wei , XU Fan , YANG Chaoshang , GENG Danmeng , ZHANG Tiejun , WANG Qian . Reproductive growth of engineered strains of Auricularia polytricha cells under Auricularia auricularia bran matrix[J]. Journal of Hebei University(Natural Science Edition), 2021 , 41(2) : 195 -200 . DOI: 10.3969/j.issn.1000-1565.2021.02.013

References

[1] 戴玉成,周丽伟,杨祝良,等.中国食用菌名录[J].菌物学报,2010,29(1):1-21.DOI:10.13346/j.mycosystema.2010.01.022.
[2] 吴芳,员瑗,刘鸿高,等.木耳属研究进展[J].菌物学报,2014,33(2): 198-207. DOI:10.13346/j.mycosystema.130282.
[3] 罗霞,余梦瑶,江南,等.毛木耳Auricularia polytricha多糖APPIIA对巨噬细胞细胞因子和iNOS基因表达的影响[J].菌物学报, 2009, 28(3): 435-439. DOI:10.13346/j.mycosystema.2009.03.020.
[4] 钟韩,杨振湖,李惠英,等.毛木耳粗多糖止血机制初探[J].中国食用菌,1991, 10(2): 14-15. DOI:10.13629/j.cnki.53-1054.1991.02.006.
[5] 周学君,俞发.毛木耳多糖的抗氧化作用[J].中国医院药学杂志,2000, 20(10): 610-611. DOI:10.3321/j.issn:1001-5213.2000.10.016.
[6] ZHAO S, RONG C B, LIU Y, et al. Extraction of a soluble polysaccharide from Auricularia polytricha and evaluation of its anti-hypercholesterolemic effect in rats[J]. Carbohydr Polym, 2015, 122: 39-45. DOI:10.1016/j.carbpol.2014.12.041.
[7] 郑林用,何希德,何权,等.毛木耳对小鼠免疫功能的影响研究[J]. 中国食用菌,2010,29(5): 43-45. DOI:10.3969/j.issn.1003-8310.2010.05.015..
[8] 阮时珍.野外蔗渣袋栽毛木耳速生高产技术[J].中国食用菌,1993,12(3): 46-47. DOI:10.13629/j.cnki.53-1054.1993.03.023.
[9] 孙召伟,邢力,王宇,等.五种菇类菌糠营养成分的比较研究[J].黑龙江农业科学,2014(9): 32-33. DOI:10.3969/j.issn.1002-2767.2014.09.008.
[10] 耿小丽,刘宇,赵爽,等.食用菌菌糠再利用研究[J].中国食用菌,2012, 31(1): 24-25. DOI:10.3969/j.issn.1003-8310.2012.01.009.
[11] ZHANG X, ZHANG X F, LI H P, et al. Atmospheric and room temperature plasma(ARTP)as a new powerful mutagenesis tool[J]. Appl Microbiol Biotechnol, 2014, 98(12): 5387-5396. DOI:10.1007/s00253-014-5755-y.
[12] 李晓宇.重铬酸钾滴定法测定土壤有机质应注意的几个问题[J]. 防护林科技,2011(3): 119. DOI:10.3969/j.issn.1005-5215.2011.03.049.
[13] 李杉杉.凯氏定氮法测定蛋白质的方法分析[J].现代食品,2018(21):140-142. DOI:10.16736/j.cnki.cn41-1434/ts.2018.21.044.
[14] 周勇辉.6种常见的蔬菜中粗纤维含量的测定[J].现代园艺,2019(1):13-14. DOI:10.3969/j.issn.1006-4958.2019.01.005.
[15] 蒋次清,王岚,廖臻,等.微波马弗炉测定烟草中灰分的研究[J].安徽农业科学,2012, 40(7): 3980, 3996. DOI:10.3969/j.issn.0517-6611.2012.07.050.
[16] 王谦,尹红芳,蔡喜存,等.毛木耳菌丝体的营养生长条件[J].河北大学学报(自然科学版), 2007,27(5): 521-524. DOI:10.3969/j.issn.1000-1565.2007.05.019.
[17] 覃晓娟,张野,仇惠君,等.以木薯秆屑为主料的毛木耳栽培配方筛选及其经济效益分析[J].西南农业学报,2017,30(2): 421-425. DOI:10.16213/j.cnki.scjas.2017.2.030. (
Options
Outlines

/

〈 〉