The factors of ammonia production process of Fe(Ⅱ)EDTA-NO reduction by Shewanella sp. RQs-106 were investigated, and the suitable operating conditions were determined. The results showed that sodium acetate and sodium citrate could not be used as electron donors of strain RQs-106, but the most suitable electron donor was sodium lactate, by which ammonia yield could reach 99% within 36 h. The Fe(Ⅱ)EDTA-NO degradation rate and ammonia production rate were affected by high or low C/N rate, and the optimal C/N mass rate was 20. Excessively high concentration of Fe(Ⅱ)EDTA-NO would inhibit the activity of the strain, which lead to the limitation in the degradation of Fe(Ⅱ)EDTA-NO. Therefore, the concentration of Fe(Ⅱ)EDTA-NO should be controlled at 1 mmol/L in this experiment. Fe(Ⅱ)EDTA-NO could not be reduced by strain RQs-106 when the initial pH was 5.0-6.0, and Fe(Ⅱ)EDTA-NO could be reduced to ammonia when the initial pH increased to 7.2-9.0. However,- DOI:10.3969/j.issn.1000-1565.2021.04.004Shewanella sp. RQs-106还原Fe(Ⅱ)EDTA-NO产氨过程影响因素高嘉欣1,路达2,张玉1,周集体1(1. 大连理工大学 环境学院,辽宁 大连 116024;2. 河北大学 化学与环境科学学院,河北 保定 071002)摘 要:考察了菌株Shewanella sp. RQs-106还原Fe(Ⅱ)EDTA-NO产氨过程的影响因素,在此基础上确定了适宜操作条件. 结果表明,乙酸钠和柠檬酸钠不能作为菌株RQs-106的电子供体,较适宜的电子供体为乳酸钠,在36 h内产氨率可达到99%. C/N质量比值过高或过低都会对菌株的Fe(Ⅱ)EDTA-NO降解率和产氨率产生影响,最适宜的C/N质量比值为20. Fe(Ⅱ)EDTA-NO浓度过高会抑制菌株活性,导致Fe(Ⅱ)EDTA-NO难以被降解,本实验条件下宜控制Fe(Ⅱ)EDTA-NO浓度为1 mmol/L. 当初始pH为5.0~6.0时,菌株RQs-106不能还原Fe(Ⅱ)EDTA-NO,当初始pH值升高到7.2~9.0时,可将Fe(Ⅱ)EDTA-NO还原为氨氮,但碱性过大对菌株RQs-106活性有抑制,较适宜的pH值为8.0. 菌株可在20~35 ℃内还原Fe(Ⅱ)EDTA-NO并产氨,最适温度为30 ℃.关键词:Fe(Ⅱ)EDTA-NO;异化硝酸盐还原为氨(DNRA);希瓦氏菌中图分类号:X172 文献标志码:A 文章编号:1000-1565(2021)04-0362-09Influencing factors of ammonia production during Fe(Ⅱ)EDTA-NO reduction by Shewanella sp. RQs-106GAO Jiaxin1,LU Da2,ZHANG Yu1,ZHOU Jiti1(1.School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China; 2.College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China)Abstract: The factors of ammonia production process of Fe(Ⅱ)EDTA-NO reduction by Shewanella sp. RQs-106 were investigated, and the suitable operating conditions were determined. The results showed that sodium acetate and sodium citrate could not be used as electron donors of strain RQs-106, but the most suitable electron donor was sodium lactate, by which ammonia yield could reach 99% within 36 h. The Fe(Ⅱ)EDTA-NO degradation rate and ammonia production rate were affected by high or low C/N rate, and the optimal C/N mass rate was 20. Excessively high concentration of Fe(Ⅱ)EDTA-NO would inhibit the activity of the strain, which lead to the limitation in the degradation of Fe(Ⅱ)EDTA-NO. Therefore, the concentration of Fe(Ⅱ)EDTA-NO should be controlled at 1 mmol/L in this experiment. Fe(Ⅱ)EDTA-NO could not be reduced by strain RQs-106 when the initial pH was 5.0-6.0, and Fe(Ⅱ)EDTA-NO could be reduced to ammonia when the initial pH increased to 7.2-9.0. However,- 收稿日期:2021-04-02 基金项目:国家自然科学基金资助项目(51578106) 第一作者:高嘉欣(1996—),女,满族,辽宁抚顺人,大连理工大学在读硕士研究生,主要从事环境微生物研究.E-mail;hahagaojx@163.com 通信作者:张玉(1973—),男,河北石家庄人,大连理工大学副教授,主要从事环境微生物研究.E-mail:zhangyu@dlut.edu.cn第4期高嘉欣等:Shewanella sp. RQs-106还原Fe(Ⅱ)EDTA-NO产氨过程影响因素the activity of strain RQs-106 was inhibited by excessive alkalinity, and the suitable pH value was 8.0, in the temperature range of 20-35 ℃, Fe(Ⅱ)EDTA-NO could be reduced by strain RQs-106 to produce ammonia, and the optimum temperature was 30 ℃.
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