In order to explore the mechanism of fulvic acid improving soybean nodulation ability, Bradyrhizobium liaoningense CCBAU 05525 and Jidou 17 were used to evaluate the effects of fulvic acid on the content and distribution of endogenous hormones by HPLC and on nodule number at the early stage of soybean nodulation. The results showed that when the concentration of fulvic acid was 500 mg/L, the number of soybean nodules was increased significantly by 73.1% at most, and the change of hormone content and distribution was the most obvious. The specific performance was that the content of zeatin(ZT), auxin(IAA)and gibberellin(GA3)in root, stem and leaf increased, the content of abscisic acid(ABA)in root decreased, and the content of ABA in leaf increased. Fulvic acid at 1 000 mg/L inhibited soybean nodulation. These results provide a theoretical basis for analyzing the mechanism of fulvic acid improving nodulation and nitrogen fixation of soybean and increasing soybean yield.
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