Glutamate chelated ferric iron(Glu-Fe(Ⅲ))and glutamate chelated ferrous iron(Glu-Fe(Ⅱ))were prepared and their iron supplementation effects were evaluated. The iron deficiency anemia(IDA)mouse model was established by combining low iron feed and bloodletting. IDA mice were randomly divided into five groups(n=5): ferrous sulfate group, commercially available iron dextran group, Glu-Fe(Ⅲ)group, Glu-Fe(Ⅱ)group and negative controls group, using normal mice as blank controls. Different iron supplements at equal iron doses were given by intragastric administration for 4 weeks. The changes of hemoglobin(HGB), red blood cells(RBC)and hematocrit(HCT)in blood were monitored by automatic hematology analyzer. The iron status of IDA mice were determined by serum iron(SI), total iron- DOI:10.3969/j.issn.1000-1565.2024.04.004谷氨酸铁螯合物对缺铁性贫血小鼠的补铁效果评价任梽源1,刘新硕1,陆时金2,杨文智1,李海鹰1(1.河北大学 药学院,河北省药物质量分析控制重点实验室,河北 保定 071002;2.中国人民解放军联勤保障部队第九六七医院 药剂科,辽宁 大连 116021)摘 要:为评价自制谷氨酸铁(Glu-Fe(Ⅲ))和谷氨酸亚铁(Glu-Fe(Ⅱ))的补铁效果,采用低铁饲料联合放血法建立缺铁性贫血(IDA)小鼠模型,并将IDA小鼠随机分成硫酸亚铁组、市售右旋糖酐铁组、Glu-Fe(Ⅱ)组和Glu-Fe(Ⅲ)组,分别灌胃给予同等铁剂量的不同补铁剂4周,并以IDA小鼠为阴性对照、正常小鼠为空白对照组进行比较.用全自动血液分析仪监测小鼠血中血红蛋白(HGB)、红细胞(RBC)和血细胞比容(HCT)变化,采用血清铁(SI)、总铁结合力(TIBC)和转铁蛋白(TRF)试剂盒测定小鼠体内铁状况并测定小鼠组织(心、肝、脾和肾)铁含量,利用总超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)试剂盒检测各种补铁剂对小鼠体内抗氧化的影响.结果显示:IDA小鼠建模成功,Glu-Fe(Ⅲ)和Glu-Fe(Ⅱ)可提高IDA小鼠的HGB、RBC和HCT值,降低TIBC和TRF水平并提升SI含量,而Glu-Fe(Ⅲ)补铁剂效果更佳.此外,相比硫酸亚铁和右旋糖酐铁,饲喂Glu-Fe(Ⅲ)在改善IDA小鼠内脏器官(心、脾和肾)肿大、促进肝恢复、提升肝脾储铁、清除组织活性氧(ROS)且增强抗氧化活性等方面表现更佳.关键词:谷氨酸亚铁螯合物;谷氨酸铁螯合物;缺铁性贫血;补铁剂中图分类号:R973 文献标志码:A 文章编号:1000-1565(2024)04-0365-08Evaluation the iron supplementation effect of glutamate chelated iron on iron deficiency anemia of miceREN Zhiyuan1, LIU Xinshuo1, LU Shijin2, YANG Wenzhi1, LI Haiying1(1. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China;2. Department of Pharmacy, Chinese 967th Hospital of the Joint Logistics Support Force of the Peoples Liberation Army, Dalian 116021, China)Abstract: Glutamate chelated ferric iron(Glu-Fe(Ⅲ))and glutamate chelated ferrous iron(Glu-Fe(Ⅱ))were prepared and their iron supplementation effects were evaluated. The iron deficiency anemia(IDA)mouse model was established by combining low iron feed and bloodletting. IDA mice were randomly divided into five groups(n=5): ferrous sulfate group, commercially available iron dextran group, Glu-Fe(Ⅲ)group, Glu-Fe(Ⅱ)group and negative controls group, using normal mice as blank controls. Different iron supplements at equal iron doses were given by intragastric administration for 4 weeks. The changes of hemoglobin(HGB), red blood cells(RBC)and hematocrit(HCT)in blood were monitored by automatic hematology analyzer. The iron status of IDA mice were determined by serum iron(SI), total iron- 收稿日期:2023-05-04;修回日期:2024-01-19 基金项目:河北省自然科学基金资助项目(C2021201026) 第一作者:任梽源(2000—),男,河北大学在读硕士研究生,主要从事药物制剂方向研究.E-mail:17835420887@163.com 通信作者:李海鹰(1973—),女,河北大学副教授,博士,主要从事药物制剂与质量控制方面研究.E-mail:lihylihy@163.com 第4期任梽源等:谷氨酸铁螯合物对缺铁性贫血小鼠的补铁效果评价河北大学学报(自然科学版) 第44卷binding capacity(TIBC)and transferrin(TRF)kits and the iron contents in mouse tissues(heart, liver, spleen and kidney)were measured. Superoxide dismutase(SOD), catalase(CAT)and malondialdehyde(MDA)kits were used to detect the effects of various iron supplements on antioxidant activity of IDA mice in vivo. The results showed that IDA mouse model was successfully established. Glu-Fe(Ⅲ)and Glu-Fe(Ⅱ)could improve the HGB, RBC and HCT values of IDA mice, meanwhile, reduce TIBC and TRF content and increase SI level for administrated IDA mice, especially Glu-Fe(Ⅲ). Compared with ferrous sulfate and iron dextran, Glu-Fe(Ⅲ)was more effective in reducing the swollen of organs(heart, spleen and kidney), improving liver recovery, enhancing iron storage in liver and spleen, scavenging tissue ROS and enhancing antioxidant activity in IDA mice.
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