In order to study the effect of baicalin on the inflammasome of mouse macrophages activated by Listeria monocytogenes(LM), the optimal concentration gradient of baicalin was determined by methylthiazolyldiphenyl-tetrazolium bromide(MTT)method. Then macrophages were treated with LM and baicalin at concentrations of 25, 50 and 100 μg/mL, respectively, to detect the expression level of inflammasome-related protein or mRNA and lactate dehydrogenase(LDH)release. The results showed that baicalin reduced IL-1β, caspase-1 p10 protein levels, LDH release and mRNA levels of NLRP3, NLRC4, NLRP10, NOD, AIM2, caspase-1, IL-1β and IL-18 after LM treatment in a dose-dependent manner. Therefore, it is speculated that baicalin can inhibit the release of inflammatory factors and cell death in macrophages by inhibiting the activation of different inflammasome in macrophages induced by LM.
LIU Wen
,
ZHANG Enhua
,
CHEN Yingying
,
ZHANG Zonghao
,
ZHANG Weiwei
,
LI Wenyan
. Inhibition of baicalin on macrophage inflammasome activation induced by Listeria monocytogenes[J]. Journal of Hebei University(Natural Science Edition), 2024
, 44(4)
: 399
-405
.
DOI: 10.3969/j.issn.1000-1565.2024.04.008
[1] DUZE S T, MARIMANI M, PATEL M. Tolerance of Listeria monocytogenes to biocides used in food processing environments[J]. Food Microbiol, 2021, 97: 103758. DOI: 10.1016/j.fm.2021.103758.
[2] DISSON O, MOURA A, LECUIT M. Making sense of the biodiversity and virulence of Listeria monocytogenes[J]. Trends Microbiol, 2021, 29(9): 811-822. DOI: 10.1016/j.tim.2021.01.008.
[3] VALENTI M, RANGANATHAN N, MOORE L S, et al. Listeria monocytogenes infections: presentation, diagnosis and treatment[J]. Br J Hosp Med, 2021, 82(10): 1-6. DOI: 10.12968/hmed.2021.0107.
[4] HANES R M, HUANG Z. Investigation of antimicrobial resistance genes in Listeria monocytogenes from 2010 through to 2021[J]. Int J Environ Res Public Health, 2022, 19(9): 5506. DOI: 10.3390/ijerph19095506.
[5] ELSAYED M E, ABD EL-HAMID M I, EL-GEDAWY A, et al. New insights into Listeria monocytogenes antimicrobial resistance, virulence attributes and their prospective correlation[J]. Antibiotics, 2022, 11(10): 1447. DOI: 10.3390/antibiotics11101447.
[6] CUI X D, ZHANG J K, SUN Y W, et al. Synergistic antibacterial activity of baicalin and EDTA in combination with colistin against colistin-resistant Salmonella[J]. Poult Sci, 2023, 102(2): 102346. DOI: 10.1016/j.psj.2022.102346.
[7] SONG L J, ZHU S M, LIU C, et al. Baicalin triggers apoptosis, inhibits migration, and enhances anti-tumor immunity in colorectal cancer via TLR4/NF-κB signaling pathway[J]. J Food Biochem, 2022, 46(3): e13703. DOI: 10.1111/jfbc.13703.
[8] GAO A J, TANG H X, ZHANG Q, et al. Mst1/2-ALK promotes NLRP3 inflammasome activation and cell apoptosis during Listeria monocytogenes infection[J]. J Microbiol, 2021, 59(7): 681-692. DOI: 10.1007/s12275-021-0638-2.
[9] LI D Y, WU M H. Pattern recognition receptors in health and diseases[J]. Signal Transduct Target Ther, 2021, 6(1): 291. DOI: 10.1038/s41392-021-00687-0.
[10] FU J, WU H. Structural mechanisms of NLRP3 inflammasome assembly and activation[J]. Annu Rev Immunol, 2023, 41: 301-316. DOI: 10.1146/annurev-immunol-081022-021207.
[11] CAI B S, ZHAO J, ZHANG Y L, et al. USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3[J]. Autophagy, 2022, 18(5): 990-1004. DOI: 10.1080/15548627.2021.1965426
[12] HANG Y Y, TAN L, CHEN Q, et al. E3 ubiquitin ligase TRIM24 deficiency promotes NLRP3/caspase-1/IL-1β-mediated pyroptosis in endometriosis[J]. Cell Biol Int, 2021, 45(7): 1561-1570. DOI: 10.1002/cbin.11592.
[13] ZHANG S Y, GUAN X M, LIU W, et al. YTHDF1 alleviates sepsis by upregulating WWP1 to induce NLRP3 ubiquitination and inhibit caspase-1-dependent pyroptosis[J]. Cell Death Discov, 2022, 8(1): 244. DOI: 10.1038/s41420-022-00872-2.
[14] TENG J F, MEI Q B, ZHOU X G, et al. Polyphyllin VI induces caspase-1-mediated pyroptosis via the induction of ROS/NF-κB/NLRP3/GSDMD signal axis in non-small cell lung cancer[J]. Cancers, 2020, 12(1): 193. DOI: 10.3390/cancers12010193.
[15] LYU H P, NI H Z, HUANG J Y, et al. VX-765 prevents intestinal ischemia-reperfusion injury by inhibiting NLRP3 inflammasome[J]. Tissue Cell, 2022, 75: 101718. DOI: 10.1016/j.tice.2021.101718.
[16] SCHLEE M, HARTMANN G. The chase for the RIG-I ligand - recent advances[J]. Mol Ther, 2010, 18(7): 1254-1262.DOI: 10.1038/mt.2010.90.
[17] ZHU H, ZHAO M, CHANG C, et al. The complex role of AIM2 in autoimmune diseases and cancers[J]. Immun Inflamm Dis, 2021, 9(3): 649-665. DOI: 10.1002/iid3.443.
[18] BARNETT K C, LI S, LIANG K, et al. A 360° view of the inflammasome: Mechanisms of activation, cell death, and diseases[J]. Cell, 2023, 186(11): 2288-2312. DOI: 10.1016/j.cell.2023.04.025. (
