To explore the function of SiNF-YA subfamily genes in Setaria italica. Firstly, the bioinformatics of 10 members of the SiNF-YA subfamily genes in foxtail millet were analyzed by using several online websites. Secondly, Ubuntu system and RStudio software were used for haplotype analysis of SiNF-YA subfamily genes. At last, through analysis of transcriptome data, the expression of SiNF-YA5 in different tissues of drought-sensitive and drought-tolerant varieties was investigated. The results showed that SiNF-YA subfamily genes of foxtail millet were distributed on four chromosomes, mainly located in the nucleus and extracellular secretion. The cis-acting elements related to abiotic stress response were distributed in the promoter region. Meanwhile, the subfamily genes were strongly correlated with drought resistance. In addition, the expression of SiNF-YA5 was detected before and after PEG treatment. There were significant differences between leaf and root expression levels. This study provide - DOI:10.3969/j.issn.1000-1565.2025.01.009谷子SiNF-YA亚家族基因的生物信息学分析与抗旱基因挖掘王春芳1,张霈涵2,史慎奎1,杨佳怡2,王玉芳1,祁东梅1(1.河北民族师范学院 生物与食品科学学院,冀北山区杂粮产业协同创新中心,河北 承德 067000;2.河北师范大学 生命科学学院,河北 石家庄 050024)摘 要:为探究谷子(Setaria italica)SiNF-YA亚家族基因的功能,首先,利用多个在线网站对谷子10个SiNF-YA亚家族基因进行了相关的生物信息学分析;其次,利用Ubuntu系统和RStudio软件对谷子SiNF-YA基因进行单倍型分析;最后,通过对转录组数据的分析,探究谷子SiNF-YA5亚家族基因在干旱敏感品种、耐旱品种各组织中的表达量差异.结果表明,谷子SiNF-YA亚家族基因分布在4条染色体上,主要定位于细胞核和胞外分泌中,其启动子区含有与非生物胁迫响应相关的顺式作用元件.同时该基因亚家族成员与抗旱性具有很强的相关性,并且谷子SiNF-YA5亚家族基因在PEG处理前后,叶片和根部的表达量具有显著性差异.本研究为谷子抗旱品种的育种提供理论支持.关键词:谷子;SiNF-YA亚家族基因;抗旱;生物信息学分析;单倍型分析中图分类号:S515 文献标志码:A 文章编号:1000-1565(2025)01-0082-09Bioinformatics analysis of SiNF-YA subfamily genes and drought resistance gene mining in Setaria italicaWANG Chunfang1, ZHANG Peihan2, SHI Shenkui1, YANG Jiayi2, WANG Yufang1, QI Dongmei1(1. Collaborative Innovation Center of Minor Cereals Industry in North Hebei, College of Biological and Food Science,Hebei Minzu Normal University, Chengde 067000,China; 2. College of Life Science, Hebei Normal University, Shijiazhuang 050024,China)Abstract: To explore the function of SiNF-YA subfamily genes in Setaria italica. Firstly, the bioinformatics of 10 members of the SiNF-YA subfamily genes in foxtail millet were analyzed by using several online websites. Secondly, Ubuntu system and RStudio software were used for haplotype analysis of SiNF-YA subfamily genes. At last, through analysis of transcriptome data, the expression of SiNF-YA5 in different tissues of drought-sensitive and drought-tolerant varieties was investigated. The results showed that SiNF-YA subfamily genes of foxtail millet were distributed on four chromosomes, mainly located in the nucleus and extracellular secretion. The cis-acting elements related to abiotic stress response were distributed in the promoter region. Meanwhile, the subfamily genes were strongly correlated with drought resistance. In addition, the expression of SiNF-YA5 was detected before and after PEG treatment. There were significant differences between leaf and root expression levels. This study provide - 收稿日期:2024-06-12;修回日期:2024-08-24 基金项目: 承德市基础研究项目(202305B093);河北民族师范学院青年基金项目(QN2019003) 第一作者:王春芳(1983—),女,河北民族师范学院副教授,博士,主要从事谷子分子育种方面的研究.E-mail:wangchunfang18@126.com 通信作者:祁东梅(1990—),女,河北民族师范学院讲师,博士,主要从事谷子功能基因方面的研究.E-mail: dmqikaka@163.com 第1期王春芳等:谷子SiNF-YA亚家族基因的生物信息学分析与抗旱基因挖掘河北大学学报(自然科学版) 第45卷theoretical support for functional verification of subsequent SiNF-YA gene subfamilies and breeding of foxtail millet varieties with drought resistance.
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