In this study, the secondary metabolites of fungus Fusarium sp. J3 were isolated and identified to explore novel compounds that possess unique structures and biological activities. The ethyl acetate extract of the rice fermentation of the fungus was purified by separation technologies such as silica gel column chromatography, gel column chromatography and high-performance liquid chromatography(HPLC), and the chemical structures of these compounds were elucidated by NMR and ESI-MS. A total of 17 compounds were isolated, including 1 aromatic compound(14), 1 amide compound(15), 3 terpenoids(16, 22, 28), 3 cyclic peptides(17, 29, 30), 1 ketene compound(18), 5 esters(19, 20, 23, 24, 25), and 3 steroids(21, 26, 27). Among these compounds, 24 was isolated from genus Fusarium for the first time. The anti-inflammatory and anti-tumor activities of these compounds were evaluated. Compounds 17, 24 and 30 showed certain anti-inflammatory effect at a mass concentration of 10 μg/mL, with the inhibition rates against NO reaching about 45%. Compound 29 displayed significant inhibitory activity- 引用格式:冯忠居,于明威,张聪,等.冲刷场地地震波形对大直径变截面单桩时程响应的影响［J］.河北大学学报(自然科学版),2026,46(4):337-348.引用格式:苏亚宁,刘惠颖,唐宏亮,等.镰刀菌属真菌Fusarium sp.J3次级代谢产物及生物活性［J］.河北大学学报(自然科学版),2026,46(4):403-412.DOI:10.3969/j.issn.1000-1565.2026.04.008镰刀菌属真菌Fusarium sp.J3次级代谢产物及生物活性苏亚宁1,刘惠颖1,唐宏亮2,王润妍1,赵晓珑1,罗都强2(1.河北大学 化学与材料科学学院,河北 保定 071002;2.河北大学 生命科学学院,河北 保定 071002)摘 要:以镰刀菌Fusarium sp.J3为研究对象,挖掘结构新颖且具有生物活性的次级代谢产物.对真菌Fusarium sp.J3进行大米发酵,通过硅胶柱层析、凝胶柱层析、高效液相色谱(HPLC)等分离技术分离发酵物的乙酸乙酯提取物,利用NMR、ESI-MS等波谱解析技术进行化合物结构鉴定.从目标菌株的乙酸乙酯提取物中共分离得到了17个化合物,包含1个芳香类化合物(14),1个酰胺类化合物(15),3个萜类化合物(16,22,28),3个环肽类化合物(17,29,30),1个烯酮类化合物(18),5个酯类化合物(19,20,23,24,25)和3个甾体类化合物(21,26,27);其中,化合物24系新化合物,且首次从镰刀菌属真菌中分离得到.对所得化合物进行了抗炎和抗肿瘤活性测定,结果表明:化合物17、24和30在质量浓度为10 μg/mL时,具有一定的抗炎活性,对NO抑制率达到45%以上;化合物29对肿瘤细胞MCF-7具有显著抑制活性,IC50值为6.77 μg/mL.关键词:Fusarium sp.J3;次级代谢产物;抗炎;抗肿瘤中图分类号:Q936 文献标志码:A 文章编号:1000-1565(2026)04-0403-10DOI:10.3969/j.issn.1000-1565.2026.04.008Secondary metabolites of fungus Fusarium sp. J3 and their bioactivitySU Yaning1, LIU Huiying1, TANG Hongliang2, WANG Runyan1, ZHAO Xiaolong1, LUO Duqiang2(1. College of Chemistry and Materials Science, Hebei University, Baoding 071002, China; 2. College of Life Sciences, Hebei University, Baoding 071002, China)Abstract: In this study, the secondary metabolites of fungus Fusarium sp. J3 were isolated and identified to explore novel compounds that possess unique structures and biological activities. The ethyl acetate extract of the rice fermentation of the fungus was purified by separation technologies such as silica gel column chromatography, gel column chromatography and high-performance liquid chromatography(HPLC), and the chemical structures of these compounds were elucidated by NMR and ESI-MS. A total of 17 compounds were isolated, including 1 aromatic compound(14), 1 amide compound(15), 3 terpenoids(16, 22, 28), 3 cyclic peptides(17, 29, 30), 1 ketene compound(18), 5 esters(19, 20, 23, 24, 25), and 3 steroids(21, 26, 27). Among these compounds, 24 was isolated from genus Fusarium for the first time. The anti-inflammatory and anti-tumor activities of these compounds were evaluated. Compounds 17, 24 and 30 showed certain anti-inflammatory effect at a mass concentration of 10 μg/mL, with the inhibition rates against NO reaching about 45%. Compound 29 displayed significant inhibitory activity- 收稿日期:2025-08-28;修回日期:2026-03-26 基金项目:河北省生物医药联合基金项目(B2020201090);省级大学生创新训练计划项目(DC2025311) 第一作者:苏亚宁(2002—),女,河北大学在读硕士研究生,主要从事天然药物化学研究.E-mail:3176926395@qq.com 通信作者:赵晓珑(1980—),女,河北大学副教授,博士,主要从事金属配合物抗癌作用和天然产物化学研究.E-mail: longlong_666@sina.com罗都强(1968—),男,河北大学教授,博士,主要从事天然产物化学研究.E-mail:duqiangluo999@126.com 第4期苏亚宁等:镰刀菌属真菌Fusarium sp.J3次级代谢产物及生物活性河北大学学报(自然科学版) 第46卷toward MCF-7 cells with IC50 value of 6.77 μg/mL.
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