河北大学学报(自然科学版) ›› 2010, Vol. 30 ›› Issue (2): 196-200.DOI: 10.3969/j.issn.1000-1565.2010.02.018

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弱碱性介质下微波法提取灵芝多糖的优化

王谦,钱先来   

  1. 河北大学生命科学学院,食药用真菌研究所,河北,保定,071002
  • 出版日期:2010-03-25 发布日期:2010-03-25
  • 基金资助:
    河北省科技支撑项目

Optimization for the Extraction of Polysaccharides by Microwave in Weak Ilkaline Medium

WANG Qian,QIAN Xian-lai   

  • Online:2010-03-25 Published:2010-03-25

摘要: 为了探寻一种高效的灵芝多糖提取方法,采用单因子分析和正交实验,对弱碱性介质下微波法提取灵芝多糖进行了优化研究.各因素对灵芝多糖提取的影响依次为提取时间,pH值,固液质量比,预浸时间.最适条件为预浸1.5 h,提取15 min,固液质量比1∶110,pH 8.0,提取2次.灵芝多糖提取率为3.200%,结果表明:该方法能显著提高灵芝多糖的提取率.

关键词: 灵芝多糖, 微波法, 正交实验

Abstract: The optimization of the extraction process the Ganoderma lucidum polyscaccharides(GLP) was studied. The microwave method under slightly alkaline medium was employed and a single factor analysis and an orthogonal test were used in the study. The order of the effects of the factors influencing the extraction was as follow: extraction time, pH, the mass ratio between G. lucidum and water, presoak time. The optimum condition was: presoak for 1.5 h, extract for 15 min, the mass ratio of G. lucidum and water 1∶110, pH 8.0 and extracted twice. GLP extraction rate of 3.200%, the result indicated that the method can significantly improve the rate of extraction of GLP.

Key words: Ganoderma lucidum polysaccharides, microwave, orthogonal test

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