金雀异黄素对小鼠黑色素瘤B16BL6细胞增殖、迁移和黏附的影响

doi:10.3969/j.issn.1000-1565.2010.04.016

摘要/Abstract

摘要： 利用四甲基偶氮唑盐微量酶反应比色法、细胞划痕实验和细胞黏附实验等研究了金雀异黄素对小鼠黑色素瘤B16BL6细胞增殖、迁移以及与血管内皮细胞黏附的影响; 利用免疫荧光实验观察了金雀异黄素对B16BL6细胞中微管和肌动蛋白分布的影响.结果表明,与对照组相比,金雀异黄素可以抑制B16BL6细胞的增殖、迁移和与血管内皮细胞的黏附,影响B16BL6细胞中肌动蛋白的组装,但不影响细胞中微管的组装.这些结果提示,金雀异黄素抑制B16BL6细胞的增殖、迁移和与血管内皮细胞的黏附可能是通过诱导细胞中肌动蛋白的重排来实现的.

关键词: 金雀异黄素, 增殖, 迁移, 黏附, B16BL6细胞

中图分类号:

R73-3

张梅. 金雀异黄素对小鼠黑色素瘤B16BL6细胞增殖、迁移和黏附的影响[J]. 河北大学学报(自然科学版), 2010, 30(4): 413-418.

ZHANG Mei. Effect of Genistein on Proliferation, Migration and Adhesion of Mouse Melanoma B16BL6 Cell[J]. Journal of Hebei University (Natural Science Edition), 2010, 30(4): 413-418.

参考文献

[1] 朱军, 沈维干, 张育, 武辉, ZHU Jun, SHEN Wei-gan, ZHANG Yu, WU Hui. 金雀异黄素抑制SGC7901细胞增殖、迁移和粘附的研究 [J]. 实用临床医药杂志 2008.doi:10.3969/j.issn.1672-2353.2008.05.008
[2] 沈维干, 朱军, 于智勇, 薛庆於, SHEN Wei-gan, ZHU Jun, YU Zhi-yong, XUE Qing-yu. 低氧对人肺腺癌A549细胞迁移和黏附的影响 [J]. 肿瘤 2008.doi:10.3781/j.issn.1000-7431.2008.03.009
[3] 金永生, 刘超美. 303 金雀异黄素的药理作用 [J]. 国外医药(植物药分册) 2002.doi:10.3969/j.issn.1674-5515.2002.05.002
[4] 朱松柏, 吕永曼. 金雀异黄素抗癌作用分子机制研究进展 [J]. 华中医学杂志 2006.
[5] ArloJ. Miller, Martin C. Mihm. Melanoma [J]. The New England journal of medicine 2006, 1(1).
[6] 沈维干, 朱军, 于智勇, 薛庆於. 成纤维细胞分泌TIMP-1抑制小鼠黑色素瘤细胞浸润和转移 [J]. 2009.
[7] POLKOWSKI K, MAZUREK A P. Biological properties of genistein.a review of in vitro and in vivo data [J]. Acta Polonica Pharmacology 2000, 57(02).
[8] Chodon D, Ramamurty N, Sakthisekaran D. Preliminary studies on induction of apoptosis by genistein on HepG2 cell line. [J]. Toxicology in vitro: an international journal published in association with BIBRA 2007, 5(5).
[9] MATSUKAWA Y, MARUI N, SAKAI T. Genistein arrests cell cycle progression at G2/M [J]. Cancer Research 1993, 53(06).
[10] Sarkar FH, Li Y. Mechanisms of cancer chemoprevention by soy isoflavone genistein. [J]. Cancer and Metastasis Reviews 2002, 3/4(3/4).
[11] Aihara M, Sugawara K, Torii S, Hosaka M, Kurihara H, Saito N, Takeuchi T. Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene. [J]. Laboratory investigation 2004, 12(12).
[12] Wei-Gan Shen, Qing-Yu Xue, Yi-Ding Wu, Ben-Shun Hu, Jun Zhu, Yu Zhang, Qing Su. Melanoma-Associated Antigen Family Protein-D1 Regulation of Tumor Cell Migration, Adhesion to Endothelium, and Actin Structures Reorganization in Response to Hypoxic Stress [J]. Cell communication & adhesion 2007, 1(1).
[13] Shen WG, Peng WX, Shao Y, Xu JF, Dai G, Zhang Y, Pan FY, Li CJ. Localization and activity of calmodulin is involved in cell-cell adhesion of tumor cells and endothelial cells in response to hypoxic stress [J]. Cell Biology and Toxicology 2007, 5(5).
[14] HILAKIVI-CLARKE L, CGO E, ONOJAFE I. Maternal exposure to genistein during pregnancy increases carcinogen-induced mammary tumorigenesis in female rat offspring [J]. Oncology Reports 1999, 6(05).
[15] Jefferson WN, Padilla-Banks E, Newbold RR. Disruption of the female reproductive system by the phytoestrogen genistein. [J]. Reproductive toxicology 2007, 3(3).
[16] Eccles SA. Parallels in invasion and angiogenesis provide pivotal points for therapeutic intervention [J]. The international journal of developmental biology 2004, 5/6(5/6).
[17] LEE H T, KAY E P. FGF-2 induced reorganization and disruption of actin cytoskeleton through PI 3-kinase, Rho, and Cdc42 in corneal endothelial cells [J]. Molecular Vision 2003, 9.
[18] LAUFFENBURGER D A, HORWITZ A F. Cell migration:a physically integrated molecular process [J]. Cell 1996, 84(03).
[19] MITCHISON T J, CRAMER L P. Actin-based cell motility and cell locomotion [J]. Cell 1996, 84(03).