中国明对虾CrusFc-2基因的重组表达及抗血清制备

doi:10.3969/j.issn.1000-1565.2011.02.015

摘要/Abstract

摘要： 利用PCR和DNA重组技术克隆了中国明对虾CrusFc-2基因的成熟肽编码序列并构建了pET-DsbA-CrusFc-2原核表达载体,转化E.coli BL21(DE3)后,经异丙基-β-D硫代半乳糖苷(IPTG)诱导得到了重组蛋白.重组蛋白经纯化后免疫新西兰大白兔制备了抗血清,Western Blot分析表明CrusFc-2蛋白主要存在于对虾血细胞中.

关键词: 中国明对虾, CrusFc-2基因, 原核表达, 抗血清

中图分类号:

柳峰松,王晓菲,李婷,郑立,谢松. 中国明对虾CrusFc-2基因的重组表达及抗血清制备[J]. 河北大学学报(自然科学版), 2011, 31(2): 181-185.

LIU Feng-song,WANG Xiao-fei,LI Ting,ZHENG Li,XIE Song. Prokaryotic Expression and Antiserum Preparation of CrusFc-2 in Chinese Shrimp,Fenneropenaeus chinensis[J]. Journal of Hebei University (Natural Science Edition), 2011, 31(2): 181-185.

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