核糖体蛋白L22对果蝇发育的影响及机制

doi:10.3969/j.issn.1000-1565.2014.01.012

摘要/Abstract

摘要： 为了研究核糖体蛋白L22对果蝇发育的影响及其机制,采用RNAi方法,分别在果蝇胚胎、幼虫、眼睛和翅膀中减少L22表达,观察相应表型的变化;S2细胞中敲除L22,统计细胞数目,并通过RT-PCR法检测与细胞凋亡和细胞周期相关基因的表达;三期幼虫翅膀disc中干扰L22,吖啶橙染色.结果表明:L22表达量减少引起胚胎死亡、幼虫发育延缓,眼睛和翅膀发育缺陷,S2细胞数目减少,与细胞凋亡和细胞周期相关基因异常表达,翅膀disc中有明显的凋亡信号.

关键词: 核糖体蛋白L22, RNAi, 凋亡, 细胞周期

Key words: Ribosomal protein L22, RNAi, apoptosis, cell cycle

中图分类号:

Q939.97

李红艳. 核糖体蛋白L22对果蝇发育的影响及机制[J]. 河北大学学报(自然科学版), 2014, 34(1): 64-69.

LI Hongyan. Effect of Ribosomal protein L22 on the development of Drosophila and its mechanism[J]. Journal of Hebei University (Natural Science Edition), 2014, 34(1): 64-69.

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