耐酸酵母的分离鉴定及在丢糟发酵中的应用

doi:10.3969/j.issn.1000-1565.2016.05.011

河北大学学报(自然科学版) ›› 2016, Vol. 36 ›› Issue (5): 517-523.DOI: 10.3969/j.issn.1000-1565.2016.05.011

耐酸酵母的分离鉴定及在丢糟发酵中的应用

李彦芹¹,崔海灏²,李春青¹,宋瑞雪²,陈亚军¹

收稿日期:2015-07-05 出版日期:2016-09-25 发布日期:2016-09-25
作者简介:李彦芹(1965—),女,河北满城人,河北大学副教授,主要从事微生物和免疫学研究. E-mail:li13102929@126.com
基金资助:
河北大学横向课题(2016-01)

Separation and identification of acid proof yeast and its application in fermentation of waste fermenting grains

LI Yanqin¹,CUI Haihao²,LI Chunqing¹,SONG Ruixue²,CHEN Yajun¹

1.College of Life Sciences, Hebei University, Baoding 071002, China; 2.Research Center of Engineering Technology, Wine Company Limited Hebei Shilixiang, Botou 062150, China

Received:2015-07-05 Online:2016-09-25 Published:2016-09-25

摘要/Abstract

摘要： 从白酒丢糟及酒醅中分离得到2株酵母菌YP2、YZ13,两株菌均能在pH 3.0条件下正常生长,经鉴定YP2为Candida属,与Candida pseudolambica(KM384061.1)相似度98%;YZ13为Pichia属,与Pichia kudriavzevii(LC014798.1)相似度100%.2株酵母(种子液体积比1∶1)应用于丢糟发酵中,接种量100 mL/kg,在鲜丢糟870 g/kg、麸皮100 g/kg、硫酸铵15 g/kg、生石灰15 g/kg,28 ℃混合固态发酵60 h,丢糟(干样)的真蛋白含量达172.30 g/kg,比发酵前丢糟(干样)提高了28.29%,丰富了丢糟饲料蛋白,为丢糟发酵和高效饲料提供了依据.

关键词: 酵母菌, 耐酸性, 真蛋白

Abstract: The two yeaststrainsYP2 and YZ13 were isolated from Chinese-spirit fermenting grains and they could all grow well under the condition of pH 3.0.Strain YP2 was identified as belonging to Candida and the similarity degree of strain YP2 and Candida pseudolambica(KM384061.1)reached 98%,while strain YZ13 to Pichia and the similarity degree with Pichia kudriavzevii(LC014798.1)reached 100%.The two strains were mixed at the ratio of 1∶1 in weight and applied to the fermentation of waste fermenting grains.The process of solid state fermentation lasted for 60 hours under the condition of inoculation quantity 100 mL/kg,fresh fermenting grains 870 g/kg,wheat bran 100 g/kg,ammonia sulfate 15 g/kg,quicklime 15 g/kg,temperature 28 ℃.The results showed that the protein content of the dry sample of waste fermenting grains reached 172.30 g/kg and increased by 28.29% and it enriched feed protein of waste fermenting grains and provided foundation for waste fermenting grains and high efficiency feed.

Key words: yeast, acid proof, true protein

中图分类号:

X172
X797

李彦芹,崔海灏,李春青,宋瑞雪,陈亚军. 耐酸酵母的分离鉴定及在丢糟发酵中的应用[J]. 河北大学学报(自然科学版), 2016, 36(5): 517-523.

LI Yanqin,CUI Haihao,LI Chunqing,SONG Ruixue,CHEN Yajun. Separation and identification of acid proof yeast and its application in fermentation of waste fermenting grains[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(5): 517-523.

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耐酸酵母的分离鉴定及在丢糟发酵中的应用

Separation and identification of acid proof yeast and its application in fermentation of waste fermenting grains

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