关键词: 生地黄, 熟地黄, 指纹图谱, HPLC, 炮制

Abstract: The quality differences of rehmanniae radix and rehmanniae radix praeparata between their ancient preparation and commercial medicines were investigated by high performance liquid chromatography(HPLC)fingerprinting technology,providing reference for the improvement of their quality criterions and the study of their preparation processes of rehmanniae radix and rehmanniae radix praeparata.The samples were extracted with methanol and analyzed by HPLC.The chromatographic conditions were as follows:Welchrom C18(4.6 mm × 250 mm,5 μm )column;acetonitrile-0.1% phosphate solution(V/V)was used as mobile phase for gradient elution;multi-wavelength UV detector;the flow rate was 1.0 mL/min and the column temperature was 35 ℃;the injection volume was 20 μL.There were significant differences between the ancient preparation and commercial medicines through the comparison of their common peaks.The involved component contents,catalpol and acteoside of rehmanniae radix and rehmanniae radix praeparata from ancient preparation were higher than those of commercial medicines;while 5-HMF from ancient preparation were much lower than those of commercial medicines.The preparation processes- DOI:10.3969/j.issn.1000-1565.2017.01.008古法炮制地黄与市售地黄成分的比较杨飞飞¹,李胜男¹,郭怀忠¹,杨茹敏²(1.河北大学 药学院,河北 保定 071002; 2.安国市益源康中药材有限公司,河北 安国 071200)摘 要:通过高效液相色谱(HPLC)指纹图谱技术来研究古法炮制生、熟地黄药材与当前市售生、熟地黄的品质差异,为地黄药材质量标准的完善以及炮制方法的研究提供参考.样品采用甲醇提取,使用Welchrom C18色谱柱(4.6 mm × 250 mm,5 μm ),乙腈-0.1%(体积分数)磷酸水溶液梯度洗脱,多波长紫外检测器检测,流速 1.0 mL/min,进样量 20 μL,柱温 35 ℃.分别对生、熟地黄的HPLC指纹图谱的共有峰面积进行比对.古法炮制地黄与市售地黄所含成分有显著差异,古法竹刀切片制生地黄与古法酒蒸炮制熟地黄,其主要成分梓醇、毛蕊花糖苷含量普遍高于市售生、熟地黄;而5-羟甲基糠醛的含量远低于市售品.关键词:生地黄;熟地黄;指纹图谱;HPLC;炮制中图分类号:O657.8 文献标志码:A 文章编号:1000-1565(2017)01-0047-09Component comparison of rehmanniae radix and rehmanniae radix praeparata between their ancient preparation and commercial medicinesYANG Feifei¹,LI Shengnan¹,GUO Huaizhong¹,YANG Rumin²( 1.College of Pharmacy,Hebei University,Baoding 071002,China;2.Yiyuankang Traditional Chinese Medicine Co.Ltd.of Anguo,Anguo 071200,China )Abstract:The quality differences of rehmanniae radix and rehmanniae radix praeparata between their ancient preparation and commercial medicines were investigated by high performance liquid chromatography(HPLC)fingerprinting technology,providing reference for the improvement of their quality criterions and the study of their preparation processes of rehmanniae radix and rehmanniae radix praeparata.The samples were extracted with methanol and analyzed by HPLC.The chromatographic conditions were as follows:Welchrom C18(4.6 mm × 250 mm,5 μm )column;acetonitrile-0.1% phosphate solution(V/V)was used as mobile phase for gradient elution;multi-wavelength UV detector;the flow rate was 1.0 mL/min and the column temperature was 35 ℃;the injection volume was 20 μL.There were significant differences between the ancient preparation and commercial medicines through the comparison of their common peaks.The involved component contents,catalpol and acteoside of rehmanniae radix and rehmanniae radix praeparata from ancient preparation were higher than those of commercial medicines;while 5-HMF from ancient preparation were much lower than those of commercial medicines.The preparation processes- 收稿日期:2016-07-06 基金项目:河北省自然科学基金资助项目(H2016201221) 第一作者:杨飞飞(1988—),女,山东济南人,河北大学在读硕士研究生.E-mail:524667972@qq.com 通信作者:郭怀忠(1968—),男,内蒙古商都人,河北大学教授,博士,主要从事中药质量控制及药效学研究.E-mail:ghuaizh@aliyun.com第1期杨飞飞等:古法炮制地黄与市售地黄成分的比较of rehmanniae radix and rehmanniae radix praeparata are necessary to be further investigated and standardized to ensure their quality and efficacy.

Key words: rehmanniae radix, rehmanniae radix praeparata, fingerprints, HPLC, preparation process