中华鳖tyrp-1基因的生物信息学分析

doi:10.3969/j.issn.1000-1565.2017.03.009

河北大学学报(自然科学版) ›› 2017, Vol. 37 ›› Issue (3): 274-280.DOI: 10.3969/j.issn.1000-1565.2017.03.009

中华鳖tyrp-1基因的生物信息学分析

王亚光¹,邵巧巧¹,孙冰冰¹,张耀红²,管越强¹

收稿日期:2016-06-15 出版日期:2017-05-25 发布日期:2017-05-25
通讯作者: 管越强(1973—),男,河北深泽人,河北大学教授,主要从事水生动物营养与免疫研究.E-mail:guanyueqiang@hbu.edu.cn
作者简介:王亚光(1990—),男,山西平定人,河北大学在读硕士研究生. E-mail:1542120049@qq.com
基金资助:
河北省自然科学基金资助项目(C2016201207);河北省现代农业产业技术体系淡水养殖创新团队项目

Bioinformatics analysis of tyrp-1 gene in Pelodiscus sinensis

WANG Yaguang¹,SHAO Qiaoqiao¹,SUN Bingbing¹,ZHANG Yaohong²,GUAN Yueqiang¹

1.College of Life Sciences, Hebei University, Baoding 071002, China; 2.Baoding Fishery Technology Extension Station, Baoding 071051, China

Received:2016-06-15 Online:2017-05-25 Published:2017-05-25

摘要/Abstract

摘要： 为阐明中华鳖酪氨酸酶相关蛋白1(TYRP-1)基因tyrp-1 及蛋白质结构与性质,利用相关软件、数据库和方法对其进行了生物信息学分析.结果显示,基因转录本全长为2 829 bp,编码535个aa. 预测TYRP-1蛋白分子质量为60.16 ku,理论等电点为5.35,为不稳定蛋白;N端含有信号肽,与信号识别蛋白结合后,促进其穿越内质网膜进入内质网腔;C端含有跨膜结构域,锚定于细胞膜上,发挥重要的生物学功能;TYRP-1蛋白含有2个铜离子结合结构域,与DCT蛋白有明显的相互作用.

关键词: 中华鳖, tyrp-1基因, 生物信息学

Abstract: In order to clarify the structure and characteristics of tyrosinase related protein 1(TYRP-1)in Chinese softshell turtle Pelodiscus sinensis, bioinformatics analysis was carried out using related softwares, databases and methods. The results showed that the transcript length of tyrp-1 gene was 2 829 bp, encoding a putative 535 aa. The deduced molecular weight of TYRP-1 protein was 60.16 ku and theoretical isoelectric point was 5.35, which indicated it was an unstable protein. A signal peptide domain was located in N-terminus, and it could bind to the signal recognition protein, leading the polypeptide chain through the endoplasmic reticulum membrane to the endoplasmic reticulum cavity. A transmembrane domain in the C-terminus, which anchored on the cell membrane, played an important biological function. The TYRP-1 protein contained two copper-binding domains, and interacted with DCT protein.

Key words: Pelodiscus sinensis, tyrp-1 gene, bioinformatics

中图分类号:

S917.4

王亚光,邵巧巧,孙冰冰,张耀红,管越强. 中华鳖tyrp-1基因的生物信息学分析[J]. 河北大学学报(自然科学版), 2017, 37(3): 274-280.

WANG Yaguang,SHAO Qiaoqiao,SUN Bingbing,ZHANG Yaohong,GUAN Yueqiang. Bioinformatics analysis of tyrp-1 gene in Pelodiscus sinensis[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(3): 274-280.

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中华鳖tyrp-1基因的生物信息学分析

Bioinformatics analysis of tyrp-1 gene in Pelodiscus sinensis

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