可产木聚糖酶的蕙兰根内生细菌的筛选与鉴定

doi:10.3969/j.issn.1000-1565.2017.05.011

河北大学学报(自然科学版) ›› 2017, Vol. 37 ›› Issue (5): 518-522.DOI: 10.3969/j.issn.1000-1565.2017.05.011

可产木聚糖酶的蕙兰根内生细菌的筛选与鉴定

马春玲,李思含,赵东芳,李广硕,马睿忆,孙磊

收稿日期:2017-06-22 出版日期:2017-09-25 发布日期:2017-09-25
通讯作者: 孙磊(1971—),女,河北唐山人,河北大学教授,博士,主要从事环境微生物学研究. E-mail: sunlei1018@126.com
作者简介:马春玲(1992—),女,河北邢台人,河北大学硕士研究生,主要从事微生物资源开发研究. E-mail: 799169587@qq.com
基金资助:
国家自然科学基金资助项目(31100002);河北省生物工程重点学科经费资助项目;河北大学大学生创新创业项目(2016063)

Screening and identification of xylanase-producing endophytic bacteria of Cymbidium faberi roots

MA Chunling, LI Sihan, ZHAO Dongfang, LI Guangshuo, MA Ruiyi, SUN Lei

1.Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, China

Received:2017-06-22 Online:2017-09-25 Published:2017-09-25

摘要/Abstract

摘要： 为了获得可产木聚糖酶的功能菌,通过刚果红染色法对592株蕙兰(Cymbidium faberi)根内生细菌进行初筛,DNS法对初筛获得的菌株进行复筛,并通过形态学、生理生化特性、(G+C)的物质的量分数及16S rRNA基因序列分析对菌株进行初步鉴定.结果筛选出31株可产木聚糖酶的菌株,占筛选总菌株数量的5.23%,其中7株产酶活性较强;复筛结果显示菌株6hRe76产木聚糖酶活力最高,其发酵液木聚糖酶酶活为57.15 U/mL,初步鉴定该菌株为克里布所类芽胞杆菌(Paenibacillus kribbensis).该研究为木聚糖酶的生产提供了潜在的新资源.

关键词: 木聚糖酶, 类芽孢杆菌, 内生细菌

Abstract: In order to obtain the xylanase-producing functional strains, 592 endophytic bacteria from Cymbidium faberi roots were screened by using the method of Kongo red dye according to the size of hydrolyzed circle. The xylanase activity of strains screened was re-screened by DNS. The xylanase-producing strain was identified based on its morphological, physiological and biochemical characteristics, mole fraction of(G+C)and 16S rRNA gene sequence analysis. The results showed that 31 xylanase-producing strains were obtained, which account for 5.23% of the total strains. Seven strains with high xylanase yields were screened through DNS method. Strain 6hRe76 with the highest enzyme activity was obtained, of which the activity of xylanase was 57.15 U/ mL. The strain 6hRe76 was identified as Paenibacillus kribbensis. The research provided the new source for xylanase production.

Key words: xylanase, Paenibacillus, endophytic bacteria

中图分类号:

Q939.13

马春玲,李思含,赵东芳,李广硕,马睿忆,孙磊. 可产木聚糖酶的蕙兰根内生细菌的筛选与鉴定[J]. 河北大学学报(自然科学版), 2017, 37(5): 518-522.

MA Chunling, LI Sihan, ZHAO Dongfang, LI Guangshuo, MA Ruiyi, SUN Lei. Screening and identification of xylanase-producing endophytic bacteria of Cymbidium faberi roots[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(5): 518-522.

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可产木聚糖酶的蕙兰根内生细菌的筛选与鉴定

Screening and identification of xylanase-producing endophytic bacteria of Cymbidium faberi roots

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