河北大学学报(自然科学版) ›› 2017, Vol. 37 ›› Issue (6): 614-620.DOI: 10.3969/j.issn.1000-1565.2017.06.009

• • 上一篇    下一篇

安吉白茶愈伤组织增殖培养及茶多酚的积累

林昱星,肖泽丰,田璧瑞,郝娜,王俊丽   

  • 收稿日期:2016-09-20 发布日期:2017-11-25
  • 通讯作者: 王俊丽(1964—),女,河北新乐人,中央民族大学教授,博士生导师,主要从事植物细胞工程及资源利用研究.E-mail:wangjunli1698@163.com
  • 作者简介:林昱星(1992—),女,新疆乌鲁木齐人,中央民族大学在读硕士研究生,主要从事生物资源保护和利用方面的研究. E-mail:1011421595 @qq.com
  • 基金资助:
    国家重大科学仪器设备开发专项(2012YQ03026108);高等学校学科创新引智计划资助项目(B08044);一流大学一流学科建设项目(ydzxxk201619)

Callus proliferation and accumulation of tea polyphenols of Anji Baicha

LIN Yuxing,XIAO Zefeng,TIAN Birui,HAO Na,WANG Junli   

  1. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China
  • Received:2016-09-20 Published:2017-11-25

摘要: 为了有效利用安吉白茶资源,以安吉白茶幼叶作为外植体,探讨了不同植物生长调节剂对愈伤组织诱导、增殖及茶多酚积累的影响.研究结果表明,TDZ有利于愈伤组织的诱导、增殖及茶多酚的积累,当TDZ的质量浓度为 1.0 mg/L时,愈伤组织的诱导率为92.1%,茶多酚的质量分数为20.6%,表儿茶素的相对含量为5.3%.

关键词: 安吉白茶, 愈伤组织, 茶多酚, GC-MS分析

Abstract: For utilization of Anji Baicha,the effects of different plant growth regulators on callus,proliferation and accumulation of tea polyphenols of Anji Baicha were investigated using leaf as explants.The results showed that TDZ was more effective for callus induction,proliferation and accumulation of tea polyphenols.The callus induction frequency reached 92.1 % when the concentration of TDZ was as high as 1.0 mg/L.The mass fraction of tea polyphenols was 20.6%,and the relative abundance of epicatechin increased to 5.3%.

Key words: Anji Baicha, callus proliferation, tea polyphenols, GC-MS analyses

中图分类号: