关键词: 荧光增白剂, 胶束增敏, 荧光分光光度法

Abstract: A simple, fast and sensitive analytical method was established for the determination of fluorescent whitening agent VBL in mask by micelle-sensitized fluorescence spectrometry. The detecting conditions were optimized. The wavelength of the excited and the emitted spectrum of VBL were 345 nm and 430 nm, respectively. The slit widths were 5nm, 5 nm. According to the spectroscopic properties of VBL and the sensitization principle of surfactant micelle, the factors that influence the fluorescence intensity of the system(such as the type of sensitizer, the concentration of sensitizer and the temperature)were investigated.- DOI:10.3969/j.issn.1000-1565.2018.04.006胶束增敏荧光法测定面膜中的荧光增白剂刘丽艳¹,白洁^1,2,韩文雅²,王亚文²,吴素焕¹,韩艳梅¹,梁翠霞²(1. 河北大学 医学综合实验中心,河北 保定 071000;2. 河北大学 公共卫生学院,河北 保定 071000)摘 要:建立简单、灵敏、快速的胶束增敏荧光法,对面膜中的荧光增白剂VBL进行定量检测.对检测条件进行选择,确定了最佳的检测条件:E_x=345 nm、E_m=430 nm、Slit=5 nm,5 nm;根据荧光增白剂的光学性质、结合表面活性剂胶束增敏原理,对影响体系荧光强度的因素(增敏试剂种类、增敏剂浓度、温度等)进行了考察,确定的最佳增敏试剂为9.20×10^-4 mol/L 的CTAB(即为临界胶束浓度),最佳实验条件为34 ℃;利用单因素分析结合正交设计对样品预处理条件(提取剂的种类、浓度、料液比,提取时间、温度等)进行了筛选,确定了荧光增白剂的最佳提取工艺:提取溶剂为体积分数90%乙醇,料液比1∶75(g∶mL),提取时间30 min,提取温度34 ℃.在最佳实验条件下,VBL+CTAB体系,在5.00×10^-7~1.20×10^-4 g/L内线性关系良好(r=0.995 8),检出限为1.91×10^-7 g/L,平均回收率为101.64%~120.60%,相对标准偏差为1.41%~8.92%.本方法检测灵敏、重现性好、结果准确可靠,可用于面膜中荧光增白剂的定量检测,为中国相关监管机构对市场面膜的质量监测提供科学依据,对维护消费者身体健康有重要意义.关键词:荧光增白剂;胶束增敏;荧光分光光度法中图分类号:O657.39 文献标志码:A 文章编号:1000-1565(2018)04-0375-10Determination of fluorescent whitening agent in mask bymicellar enhanced fluorescence spectrometryLIU Liyan¹, BAI Jie^1,2,HAN Wenya², WANG Yawen², WU Suhuan¹, HAN Yanmei¹,LIANG Cuixia²(1.Medical Comprehensive Experimental Center, Hebei University, Baoding 071000, China;2. College of Public Health, Hebei University, Baoding 071000, China)Abstract: A simple, fast and sensitive analytical method was established for the determination of fluorescent whitening agent VBL in mask by micelle-sensitized fluorescence spectrometry. The detecting conditions were optimized. The wavelength of the excited and the emitted spectrum of VBL were 345 nm and 430 nm, respectively. The slit widths were 5nm, 5 nm. According to the spectroscopic properties of VBL and the sensitization principle of surfactant micelle, the factors that influence the fluorescence intensity of the system(such as the type of sensitizer, the concentration of sensitizer and the temperature)were investigated.- 收稿日期:2017-10-20 基金项目:国家自然科学基金资助项目(21605035);河北省自然科学基金资助项目(B2014201162);保定市科学技术与发展指导计划项目(12ZF054);河北大学中西部提升综合实力专项资金资助项目(20150505);河北大学实验室开放基金资助项目(sy201672);河北大学医学部第一批教育教学改革研究课题项目(YXB01-F-05) 第一作者:刘丽艳(1984—),女,河北唐山人,河北大学实验师,主要从事荧光分析、生化分析、生物传感器等研究. E-mail:liyanliu. ok@163.com 通信作者:白洁(1980—),女,河北保定人,回族,河北大学副教授,博士,主要从事荧光分析、生化分析、生物传感器等研究. E-mail:bj0427@126.com韩艳梅(1964—),女,河北保定人,河北大学教授,主要从事生理学方面研究.E-mail:hanyanmei6465@sina.com第4期刘丽艳等:胶束增敏荧光法测定面膜中的荧光增白剂According to the various factors, the optimal experimental conditions were that the sensitizer was CTAB,the concentration of CTAB was 9.20×10^-4 mol/L(critical micelle concentrations, CMC)and the temperature was 34 ℃. Through single-factor and orthogonal experiments, the sample pretreatment methods for the VBL of the facial masks were optimized. The extraction solvent was 90% ethanol, the solid-liquid ratio was 1∶75(g∶mL), the extracting time was 30 min, and the extracting temperature was 34 ℃. The result showed that the fluorescence enhancement value of the CTAB was linearly proportional to the concentration of VBL in the range of 5.00×10^-7~1.20×10^-4 g/L.The limit of detection was found to be 1.91×10^-7 g/L,the average recovery was 101.64%~120.60%, and the standard deviation was 1.41%~8.92%. This method is sensitive, reproducible and accurate, and suitable for quantitative detection of VBL in facial mask. The method can provide the scientific basis for the quality monitoring of the market mask in China’s relevant regulatory agencies, and is of great significance to the maintenance of consumer health.

Key words: fluorescent whitening agent, micellar enhanced, fluorescence spectrophotometry