保定城区2种棺头蟋携带的Wolbachia检测和系统进化

doi:10.3969/j.issn.1000-1565.2018.05.013

河北大学学报(自然科学版) ›› 2018, Vol. 38 ›› Issue (5): 526-532.DOI: 10.3969/j.issn.1000-1565.2018.05.013

保定城区2种棺头蟋携带的Wolbachia检测和系统进化

关蓓,柴金艳,周志军

收稿日期:2018-05-19 出版日期:2018-09-25 发布日期:2018-09-25
通讯作者: 周志军(1980—),男,山西长治人,河北大学副教授,主要从事直翅目昆虫分子系统进化研究.E-mail: zhijunzhou@163.com; zhijunzhou@hbu.edu.cn
作者简介:关蓓(1989—),女,山东烟台人,河北大学硕士研究生,主要从事昆虫分子生态学研究. E-mail:beijiaerdegua@163.com
基金资助:
国家自然科学基金资助项目(31471985);2016年实验室开放项目(sy201660)

Detection and phylogeny of Wolbachia in two Loxoblemmus species in Baoding urban area

GUAN Bei, CHAI Jinyan, ZHOU Zhijun

Key Laboratory of Invertebrate Systematics and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, China

Received:2018-05-19 Online:2018-09-25 Published:2018-09-25

摘要/Abstract

摘要： 利用wsp 基因特异引物(81F/691R)对河北保定城区2种棺头蟋——多伊棺头蟋Loxoblemmus doenitzi Stein, 1881 和石首棺头蟋L. equestris Saussure, 1877的头、消化道、后足肌肉和性腺携带的Wolbachia情况进行检测.结果发现,多伊棺头蟋L. doenitzi(26/33)和石首棺头蟋L. equestris(22/22)携带Wolbachia的比例很高,且头和后足肌肉检出率明显高于消化道和性腺.多伊棺头蟋L. doenitzi 和石首棺头蟋L. equestris携带的Wolbachia分别属于 B 组和 A 组,且各包括2个不同亚组.本研究为进一步研究Wolbachia对棺头蟋生物学的影响奠定了基础.

关键词: 棺头蟋, Wolbachia, Wolbachia表面蛋白

Abstract: Wolbachia screening from various tissues of two Loxoblemmus species was performed, including heads, alimentary canal, foot muscles, and gonads. DNA was extracted from these tissues, amplified using Wolbachia surface protein gene(wsp)specific primers(81F/691R)followed by sequencing and phylogenetic analysis. All two Loxoblemmus species harbored Wolbachia. The detection ratio of heads and foot muscles apparently higher than alimentary canal and gonads. L. doenitzi and L. equestris presented infection with Wolbachia B and A group, respectively. There were two Wolbachia strains from markedly different subgroup infecting L. doenitzi and L. equestris. These results provide the basis for future study on the effects of Wolbachia on the biology of two Loxoblemmus species.

Key words: Loxoblemmus, Wolbachia, Wolbachia wsp

中图分类号:

Q951

关蓓,柴金艳,周志军. 保定城区2种棺头蟋携带的Wolbachia检测和系统进化[J]. 河北大学学报(自然科学版), 2018, 38(5): 526-532.

GUAN Bei, CHAI Jinyan, ZHOU Zhijun. Detection and phylogeny of Wolbachia in two Loxoblemmus species in Baoding urban area[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(5): 526-532.

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保定城区2种棺头蟋携带的Wolbachia检测和系统进化

Detection and phylogeny of Wolbachia in two Loxoblemmus species in Baoding urban area

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