关键词: 荆芥梗, 荆芥穗, 重金属分布, 原子吸收光谱法, Pb、Cd、Ni含量测定

Abstract: The contents of lead(Pb), cadmium(Cd)and nickel(Ni)in different medicinal parts of Schizonepeta tenuifolia Briq.(STE)were determined. Meanwhile,the contamination status of STE was compared and analyzed.After pretreatment by wet digestion system,Pb, Cd and Ni were determined by graphite furnace atomic absorption spectrometry(GFAAS).2%NH₄H₂PO₄-1.67%Mg(NO₃)₂ were used as a matrix modifier to determine Pb and Cd; and 0.2%Pd(NO₃)₂-2%NH₄H₂PO₄ was used as a matrix modifier to determine Ni. The determination condition was as follows.The wavelengths of Pb,Cd,Ni were 283.3,228.8, and 232.0 nm; lamp currents were 10, 6,- DOI:10.3969/j.issn.1000-1565.2019.02.007荆芥不同部位3种重金属元素含量的比较分析步艳艳¹,任红敏²,刘晓慧²,刘廷辉³,赵燕燕¹(1.河北大学 药学院,河北省药物质量分析控制重点实验室,河北 保定 071002;2.中华人民共和国保定海关,河北 保定 071051;3.河北农业大学 植物保护学院,河北 保定 071001)摘 要:测定荆芥药材不同药用部位中铅(Pb)、镉(Cd)、镍(Ni)3种重金属元素的含量,比较分析荆芥不同部位的污染状况.对荆芥样品进行湿法消解,采用石墨炉原子吸收光谱法(GFAAS)测定样品中Pb、Cd、Ni含量,以质量分数2%磷酸二氢铵-1.67%硝酸镁作为基体改进剂测定Pb、Cd;以质量分数0.2%硝酸钯-2%磷酸二氢铵作为基体改进剂测定Ni.Pb、Cd、Ni的测定波长分别为283.3、228.8和232.0 nm,灯电流分别为10、6和25 mA;狭缝分别为0.7、0.7和0.2 L.Pb、Cd、Ni元素线性关系分别为Y=0.002 8X+0.002 7(r=0.998 9)、Y=0.034 8X+0.005 4(r=0.998 3)和Y=0.002 9X+0.004 3(r=0.998 4),回收率为98.2%~102.9%;荆芥梗中Pb、Cd和Ni的质量分数测定结果分别为0.55~0.95、0.05~0.11和0.1~1.22 mg/kg;荆芥穗质量分数测定结果分别为0.58~1.73、0.03~0.14和0.18~1.42 mg/kg.重金属元素Pb、Cd、Ni检测方法可靠,随着采收时间延长,重金属元素易由荆芥梗向荆芥穗中富集,为荆芥重金属限度质量标准制定以及临床用药部位的选择提供参考.关键词:荆芥梗;荆芥穗;重金属分布;原子吸收光谱法;Pb、Cd、Ni含量测定中图分类号:TKR917 文献标志码:A 文章编号:1000-1565(2019)02-0152-07Comparative analysis of three heavy metal elements in differentparts of Schizonepeta tenuifolia Briq.BU Yanyan¹,REN Hongmin²,LIU Xiaohui²,LIU Tinghui³,ZHAO Yanyan¹(1.Key Laboratory of Drug Quality Analysis and Control of Hebei Province,College of Pharmaceutical Sciences,Baoding 071002,China;2.Baoding Customs District P.R.China,Baoding 071051,China;3.College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China)Abstract:The contents of lead(Pb), cadmium(Cd)and nickel(Ni)in different medicinal parts of Schizonepeta tenuifolia Briq.(STE)were determined. Meanwhile,the contamination status of STE was compared and analyzed.After pretreatment by wet digestion system,Pb, Cd and Ni were determined by graphite furnace atomic absorption spectrometry(GFAAS).2%NH₄H₂PO₄-1.67%Mg(NO₃)₂ were used as a matrix modifier to determine Pb and Cd; and 0.2%Pd(NO₃)₂-2%NH₄H₂PO₄ was used as a matrix modifier to determine Ni. The determination condition was as follows.The wavelengths of Pb,Cd,Ni were 283.3,228.8, and 232.0 nm; lamp currents were 10, 6,- 收稿日期:2018-09-20 基金项目:河北检验检疫局科技计划项目(HE2017K011); 河北省自然科学基金资助项目(H2013201203);河北省重点研发计划项目(17226404D) 第一作者:步艳艳(1992—),女,河北衡水人,河北大学在读硕士研究生,主要从事药学方面的研究.E-mail:1914172479@qq.com 通信作者:赵燕燕(1960—),女,天津市人,河北大学教授,主要从事药学以及将现代分离技术用于临床、药学、食品、环境等方面的研究工作.E-mail:zhaoyany606@163.com第2期步艳艳等:荆芥不同部位3种重金属元素含量的比较分析and 25 mA, respectively.Entrance slits were 0.7, 0.7, and 0.2 L, respectively.Calibration curves of three elements( Pb,Cd,Ni)were Y=0.002 8X+0.002 7(r=0.998 9),Y=0.034 8X+0.005 4(r=0.998 3),Y=0.002 9X+0.004 3(r=0.998 4),respectively.The recovery values were 98.2%-102.9%.The results of content determination of STE’s stem were 0.55-0.95,0.05-0.11 and 0.1-1.22 mg/kg, respectively. The results of content determination of STE’s ears were 0.58-1.73,0.03-0.14 and 0.18-1.42 mg/kg.The detection methods of heavy metal elements Pb, Cd and Ni are reliable. With the prolongation of harvest time, heavy metal elements are easily enriched from STE’s stem to STE’s ears. This paper provides a reference for the development of limit standards of heavy metals in STE and the selection of clinical drug sites.

Key words: stems of Schizonepeta tenuifolia Briq., ears of Schizonepeta tenuifolia Briq., the analysis of heavy metals contents, atomic absorption spectrometry, determination of Pb,Cd and Ni