紫色红曲霉的微波诱变及其产红曲色素发酵条件的优化

doi:10.3969/j.issn.1000-1565.2020.06.009

河北大学学报(自然科学版) ›› 2020, Vol. 40 ›› Issue (6): 618-624.DOI: 10.3969/j.issn.1000-1565.2020.06.009

紫色红曲霉的微波诱变及其产红曲色素发酵条件的优化

武金霞¹,王琰¹,谭政浩¹,刘爽¹,隋思强¹,张贺迎²

收稿日期:2019-08-08 发布日期:2021-01-10
通讯作者: 国家自然科学基金资助项目(31573227)
作者简介:武金霞(1966—),女,河北保定人,河北大学教授,博士,主要从事微生物学及生物化学研究. E-mail:wjx6@163.com

Microwave mutagenesis of Monascus purpureus and optimization of fermentation conditions for Monascus pigment production

WU Jinxia¹, WANG Yan¹, TAN Zhenghao¹, LIU Shuang¹, SUI Siqiang¹, ZHANG Heying²

1. School of Life Sciences, Hebei University, Baoding 071002, China; 2.Research Center for Biotechnology, Hebei University, Baoding 071002, China

Received:2019-08-08 Published:2021-01-10

摘要/Abstract

摘要： 以紫色红曲霉S1为出发菌株进行微波诱变,筛选出产红曲色素能力最高的突变株紫色红曲霉S1-29,其红曲色素值达3 240 U/g,比出发菌株提高了57%.针对菌株S1-29固态发酵培养产红曲色素的条件进行优化,添加不同的碳源、氮源以及无机盐,研究添加物对产红曲色素能力的影响,并进行正交实验,得到最佳发酵条件:在大米基质中添加葡萄糖、蛋白胨、硫酸镁和磷酸氢二钾,使其质量分数分别为4%,6%,0.4%和0.3%,32 ℃培养11 d,菌株S1-29红曲色素值达到4 003 U/g,比优化前提高23.55%.

关键词: 紫色红曲霉, 红曲色素, 发酵优化

Abstract: The microwave mutagenesis was carried out when Monascus purpureus S1 was used as the original strain. M.purpureus S1-29 was screened with the highest Monascus pigment value of 3 240 U/g dry base, which was increased by 57% compared with that of the original strain. The conditions for the production of Monascus pigment by solid-state fermentation of strain S1-29 were optimized. The effects of carbon sources, nitrogen sources and inorganic salts on pigment production were investigated and orthogonal experiments were carried out. The results showed that when 4% glucose, 6% peptone, 0.4% magnesium sulfate and 0.3% dipotassium hydrogen phosphate were added into the rice media and cultured at 32 ℃ for 11 days, the Monascus pigment value of M.purpureus S1-29 reached 4 003 U/g dry base, which was increased by 23.55% compared with that of control.

Key words: Monascus purpureus, Monascus pigment, fermentation optimization

中图分类号:

Q939.97

武金霞,王琰,谭政浩,刘爽,隋思强,张贺迎. 紫色红曲霉的微波诱变及其产红曲色素发酵条件的优化[J]. 河北大学学报(自然科学版), 2020, 40(6): 618-624.

WU Jinxia, WANG Yan, TAN Zhenghao, LIU Shuang, SUI Siqiang, ZHANG Heying. Microwave mutagenesis of Monascus purpureus and optimization of fermentation conditions for Monascus pigment production[J]. Journal of Hebei University(Natural Science Edition), 2020, 40(6): 618-624.

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紫色红曲霉的微波诱变及其产红曲色素发酵条件的优化

Microwave mutagenesis of Monascus purpureus and optimization of fermentation conditions for Monascus pigment production

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