木贼镰刀菌Fusarium equiseti次级代谢产物的分离鉴定

doi:10.3969/j.issn.1000-1565.2023.05.010

河北大学学报(自然科学版) ›› 2023, Vol. 43 ›› Issue (5): 512-516.DOI: 10.3969/j.issn.1000-1565.2023.05.010

木贼镰刀菌Fusarium equiseti次级代谢产物的分离鉴定

潘华洋¹,刘华广¹,齐泽欣¹,赵晓珑^1,2,罗都强^1,2

收稿日期:2022-10-21 出版日期:2023-09-25 发布日期:2023-10-25
通讯作者: 赵晓珑(1980—)
作者简介:潘华洋(2000—),男,河南许昌人,河北大学在读硕士研究生,主要从事生物化学研究.
E-mail:P13536323869@163.com
基金资助:
河北省生物医药联合基金资助项目(B2020201090);河北大学大学生创新创业训练计划项目(2022231)

Isolation and identification of secondary metabolites of Fusarium equiseti

PAN Huayang¹, LIU Huaguang¹, QI Zexin¹, ZHAO Xiaolong^1,2, LUO Duqiang^1,2

1. College of Chemistry and Materials Science, Hebei University, Baoding 071002, China; 2. College of Life Sciences, Hebei University, Baoding 071002, China

Received:2022-10-21 Online:2023-09-25 Published:2023-10-25

摘要/Abstract

摘要： 为发现具有新结构和显著药理作用的化合物,以异色瓢虫内生真菌木贼镰刀菌Fusarium equiseti为研究对象,采用现代分离手段,从其次级代谢产物中分离鉴定出3种化合物1、2和3. 其中化合物1(equisetin)和2(epi-equisetin)是一对差向异构体,3(6-methoxy-droperylene)属于甾体类. 体外蛋白质酪氨酸磷酸酶1B(PTP1B)抑制活性测试结果表明,化合物1和2对PTP1B具有一定的抑制活性,IC₅₀值分别为(8.73 ± 0.03)、(12.88 ± 0.16)μg/mL. 为新型PTP1B抑制剂的研发提供了潜在的药物先导化合物.

关键词: 昆虫内生真菌, 结构鉴定, 活性测试

Abstract: In order to find compounds with new structures and significant pharmacological effects, three compounds 1, 2 and 3 were isolated and identified from the secondary metabolites of the endophytic fungus Fusarium equiseti from Harmonia axyridis by modern isolation methods. Among them, compounds 1(equisetin)and 2(epi-equisetin)are a pair of epiisomers, and 3(6-methoxy-droperylene)belongs to steroids. In vitro protein tyrosine phosphatase 1B(PTP1B)inhibitory activity test showed that compounds 1 and 2 had certain inhibitory activity on PTP1B, and IC₅₀ values were(8.73 ± 0.03),(12.88 ± 0.16)μg/mL. It provides potential drug lead compounds for the development of new PTP1B inhibitors.

Key words: insect endophytic fungi, structure identification, activity test

中图分类号:

Q936

潘华洋,刘华广,齐泽欣,赵晓珑,罗都强. 木贼镰刀菌Fusarium equiseti次级代谢产物的分离鉴定[J]. 河北大学学报(自然科学版), 2023, 43(5): 512-516.

PAN Huayang, LIU Huaguang, QI Zexin, ZHAO Xiaolong, LUO Duqiang. Isolation and identification of secondary metabolites of Fusarium equiseti[J]. Journal of Hebei University(Natural Science Edition), 2023, 43(5): 512-516.

参考文献

[1] 杨会祥, 邓园园, 李薇, 等. 昆虫内生真菌Chaetomium globosum ZWC5的分离鉴定及其抗癌活性代谢产物的研究[J]. 湖南师范大学自然科学学报, 2016, 39(4): 17-22, 95.
[2] 汪福源, 顾觉奋, 高向东, 等. 动物内生真菌研究进展[J]. 国外医药(抗生素分册), 2017, 38(2): 58-61. DOI:10.13461/j.cnki.wna.004992.
[3] 张清清, 郭玲芝, 陈剑锋, 等. 昆虫内生真菌Alternaria sp.次级代谢产物研究[J]. 三峡大学学报(自然科学版), 2015, 37(5): 110-112. DOI:10.13393/j.cnki.issn.1672-948X.2015.05.025.
[4] LIU S, ZHAO Y P, HEERING C, et al. Sesquiterpenoids from the endophytic fungus rhinocladiella similis[J]. J Nat Prod, 2019, 82(5): 1055-1062. DOI:10.1021/acs.jnatprod.8b00938.
[5] LIU H X, TAN H B, CHEN Y C, et al. Cytorhizins A-D, four highly structure-combined benzophenones from the endophytic fungus cytospora rhizophorae[J]. Org Lett, 2019, 21(4): 1063-1067. DOI:10.1021/acs.orglett.8b04107.
[6] WANG H H, LI G, QIAO Y N, et al. Chamiside A, a cytochalasan with a tricyclic core skeleton from the endophytic fungus chaetomium nigricolor F5[J]. Org Lett, 2019, 21(9): 3319-3322. DOI:10.1021/acs.orglett.9b01065.
[7] LI S J, JIAO F W, LI W, et al. Cytotoxic xanthone derivatives from the mangrove-derived endophytic fungus Peniophora incarnata Z4[J]. J Nat Prod, 2020, 83(10): 2976-2982. DOI:10.1021/acs.jnatprod.0c00523.
[8] ZU W Y, TANG J W, HU K, et al. Chaetolactam A, an azaphilone derivative from the endophytic fungus chaetomium sp. g1[J]. J Org Chem, 2021, 86(1): 475-483. DOI:10.1021/acs.joc.0c02214.
[9] WANG X, LUO X, GAN X, et al. Analysis of regulating activities of 5'-epiequisetin on proliferation, apoptosis, and migration of prostate cancer cells in vitro and in vivo[J]. Front Pharmacol, 2022, 13: 920554. DOI:10.3389/fphar.2022.920554.
[10] LIU S S, GAO W B, YANG X H, et al. Structure determination and cytotoxic evaluation of metabolites from the entomogenous fungus Fusarium equiseti[J]. J Antibiot, 2021, 74: 176-180. DOI:10.1038/s41429-020-00379-x.
[11] LU S Y, SUN Z O, HU W L, et al. PD-L1 positively regulates MET phosphorylation through inhibiting PTP1B[J]. Cancer Sci, 2021, 112(5): 1878-1887. DOI:10.1111/cas.14844.
[12] 王娟, 王辂, 常敏, 等. 红海榄内生真菌AGR12及其抑菌活性代谢产物[J]. 中国抗生素杂志, 2011, 36(2): 102-106. DOI:10.13461/j.cnki.cja.004749. (

木贼镰刀菌Fusarium equiseti次级代谢产物的分离鉴定

Isolation and identification of secondary metabolites of Fusarium equiseti

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	段慧云,赵莲,陈华. 马齿苋Portulaca oleracea的化学成分[J]. 河北大学学报(自然科学版), 2011, 31(4): 389-392.
[2]	任晓,董悦生,张华,路新华,郑智慧,刘梅,贺建功. 微生物来源的醛糖还原酶抑制剂F01-195A的研究[J]. 河北大学学报(自然科学版), 2006, 26(1): 7-10.