河北大学学报(自然科学版) ›› 2002, Vol. 22 ›› Issue (2): 186-188.DOI: 10.3969/j.issn.1000-1565.2002.02.020
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王会文,吴琛,赵晓瑜,王建辉,张瑞英,王彦芳
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摘要： 为了简化PCR模板DNA的制备,选取4种实验材料,采用SDS一步法制备PCR模板.经PCR扩增,获得同常规方法制备的模板相近的实验结果.实现了"一管一步"制备模板DNA,从而开辟了一种快速、简易、低成本的PCR模板制备方法.
关键词: PCR, DNA抽提, SDS
Abstract: The structural properties and superconductivity of Mg1-xNaxB2 compounds are reported in this paper. Bulk samples X- ray diffraction (XRD) results indicate that the samples crystallize in a hexagonal structure. Due to the chemical activity of Mg powders, a sample amount of MgO impurity phase is detected by X-ray diffraction. Magnetization measurement results indicate that the non- stoichiometry of MgB2 has no influence on the superconducting transition temperature and the transition temperature width. And the introduction of Na almost has no effect on the transition temperature.
Key words: Mg1 - x NaxB2, superconductivity, XRD
中图分类号:
Q781
王会文,吴琛,赵晓瑜,王建辉,张瑞英,王彦芳. SDS一步法制备PCR模板[J]. 河北大学学报(自然科学版), 2002, 22(2): 186-188.
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