大孔吸附树脂纯化新疆两色金鸡菊总皂苷

doi:10.3969/j.issn.1000-1565.2016.03.005

河北大学学报(自然科学版) ›› 2016, Vol. 36 ›› Issue (3): 242-248.DOI: 10.3969/j.issn.1000-1565.2016.03.005

大孔吸附树脂纯化新疆两色金鸡菊总皂苷

姚新成^1,2,张婷¹,唐辉^1,2,陈文^1,2

收稿日期:2015-08-20 出版日期:2016-05-25 发布日期:2016-05-25
通讯作者: 陈文(1967—),男,湖南益阳人,石河子大学教授,博士,主要从事药物制剂及新药研究.E-mail:chen-wen2000@126.com
作者简介:姚新成(1973—),男,江苏睢宁人,石河子大学副教授,博士,主要从事中药与天然产物活性研究. E-mail:yxc@whu.edu.cn
基金资助:
新疆生产兵团重点领域科技攻关项目(2014BA031);石河子大学高层次人才科研启动项目(RCZX201328);国家“重大新药创制”科技重大专项(2011ZX09401-007)

Purification of total saponins from Coreopsis tinctoria Nutt.in Xinjiang by macroporous adsorptive resin

YAO Xincheng^1,2,ZHANG Ting¹,TANG Hui^1,2,CHEN Wen^1,2

1.School of Pharmacy, Shihezi University, Shihezi 832000, China; 2.Ministry of Education KeyLaboratory of Xinjiang Endemic Phytomedicine Resources, Shihezi University, Shihezi 832000, China

Received:2015-08-20 Online:2016-05-25 Published:2016-05-25

摘要/Abstract

摘要： 利用大孔吸附树脂纯化新疆两色金鸡菊总皂苷.采用分光光度法测定总皂苷含量,以静态吸附量和解吸率、动态吸附量和解吸率为指标,选择合适的大孔吸附树脂和纯化条件.D-101型大孔吸附树脂对新疆两色金鸡菊总皂苷具有较强的吸附性能和较好的解吸能力.两色金鸡提取液经D-101型大孔吸附树脂处理,可获得质量分数为(40.53±0.81)%(n=3)的总皂苷部位.D-101型大孔吸附树脂可有效地纯化新疆两色金鸡菊总皂苷.

关键词: 两色金鸡菊, 总皂苷, 大孔吸附树脂

Abstract: To investigate the purification process of total saponins from Coreopsis tinctoria Nutt.using macroporous adsorptive resin.The assay of total saponins was determined by UV-Vis spectrophotometry,and the resins were selected using the parameters,such as static adsorption capacity,desorption ratio,dynamic adsorption capacity,and as well as dynamic desorption ratio.The D-101 macroporous resin is suitable for purification of total saponins from Coreopsis tinctoria Nutt.according to its adsorption and desorption ability for total saponins.The total saponins content is(40.53±0.81)%(n=3)in total solids through D-101 macroporous resin purification.The D-101 macroporous resin can be used for purification of total saponins obtained from Coreopsis tinctoria Nutt.in Xinjiang.

Key words: Coreopsis tinctoria Nutt., total saponins, macroporous adsorptive resin

中图分类号:

TQ461

姚新成,张婷,唐辉,陈文. 大孔吸附树脂纯化新疆两色金鸡菊总皂苷[J]. 河北大学学报(自然科学版), 2016, 36(3): 242-248.

YAO Xincheng,ZHANG Ting,TANG Hui,CHEN Wen. Purification of total saponins from Coreopsis tinctoria Nutt.in Xinjiang by macroporous adsorptive resin[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(3): 242-248.

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大孔吸附树脂纯化新疆两色金鸡菊总皂苷

Purification of total saponins from Coreopsis tinctoria Nutt.in Xinjiang by macroporous adsorptive resin

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