一种新型的焦磷酸根可视化检测方法

doi:10.3969/j.issn.1000-1565.2017.02.007

河北大学学报(自然科学版) ›› 2017, Vol. 37 ›› Issue (2): 147-154.DOI: 10.3969/j.issn.1000-1565.2017.02.007

一种新型的焦磷酸根可视化检测方法

苏荟娟,赵旭东,张丽,樊雯洁,王愈聪,贾红霞

收稿日期:2016-11-21 出版日期:2017-03-25 发布日期:2017-03-25
通讯作者: 王愈聪(1979—),女,山西河曲人,河北大学副教授,博士,主要从事生物功能材料的制备及应用研究.E-mail:15931795521@163.com贾红霞(1980—),女,河北宣化人,河北大学讲师,博士,主要从事生化分析及光谱分析研究.E-mail:jia123renren@126.com
作者简介:苏荟娟(1992—),女,河北邢台人,河北大学在读硕士研究生,主要从事发光材料在生物分子上的应用研究. E-mail:suhuijuan2016@163.com
基金资助:
河北省自然科学基金资助项目(B2015201130);河北大学2016校级创新创业计划项目(149)

A new method for visual detection of pyrophosphate

SU Huijuan,ZHAO Xudong,ZHANG Li,FAN Wenjie,WANG Yucong,JIA Hongxia

College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China

Received:2016-11-21 Online:2017-03-25 Published:2017-03-25

摘要/Abstract

摘要： 焦磷酸根(PPi)在生命体系和环境体系中都占有重要的地位,利用Cu²⁺诱导CdTe量子点产生荧光谱带红移的现象和PPi对铜离子的配位作用,建立了一种新型的PPi可视化检测方法.结果表明,PPi在5~50 μmol/L的浓度内可通过CdTe量子点荧光颜色的变化进行可视化检测,其他阴离子对PPi测定均无干扰.此外,利用该方法对焦磷酸酶的活性进行了检测,初步取得了满意的结果,说明本文所建立的方法可以用于PPi相关物质的检测.

关键词: 焦磷酸根, CdTe量子点, Cu²⁺, 可视化

Abstract: Pyrophosphate(PPi)plays an important role in the biological and environmental systems.Therefore,a simple,rapid,high sensitivity determination of the pyrophosphate is of great significance.A new colorimetric strategy for the visual detection of pyrophosphate by the naked eye under the irradiation of a UV lamp was developed on the basis of Cu²⁺-induced fluorescence band shift of CdTe quantum dots(QDs)and the strong interaction between PPi and Cu²⁺. PPi can be visually detected in the range of 5 μmol/L to 50 μmol/L.Furthermore,this method was of good specificity and it also can be used for the detection of the pyrophosphatase activity.

Key words: pyrophosphate, CdTe quantum dots, Cu²⁺, visual detection

中图分类号:

苏荟娟,赵旭东,张丽,樊雯洁,王愈聪,贾红霞. 一种新型的焦磷酸根可视化检测方法[J]. 河北大学学报(自然科学版), 2017, 37(2): 147-154.

SU Huijuan,ZHAO Xudong,ZHANG Li,FAN Wenjie,WANG Yucong,JIA Hongxia. A new method for visual detection of pyrophosphate[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(2): 147-154.

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一种新型的焦磷酸根可视化检测方法

A new method for visual detection of pyrophosphate

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