TNF-α对大鼠皮层神经元钠通道电流的影响

doi:10.3969/j.issn.1000-1565.2018.03.008

河北大学学报(自然科学版) ›› 2018, Vol. 38 ›› Issue (3): 278-283.DOI: 10.3969/j.issn.1000-1565.2018.03.008

TNF-α对大鼠皮层神经元钠通道电流的影响

张伟伟, 张晗, 曹芳, 穆淑梅, 康现江

收稿日期:2017-10-25 出版日期:2018-05-25 发布日期:2018-05-25
通讯作者: 康现江(1964—),男,河北邯郸人,河北大学教授,博士,主要从事生殖发育研究.E-mail:xjkang218@126.com
作者简介:张伟伟(1980—),女,河北保定人,河北大学在读博士研究生,主要从事细胞生物学研究. E-mail: zhangweiwei827@163.com
基金资助:
国家自然科学基金资助项目(31572269);生物学河北省国家重点学科培育项目;河北省教育厅资助项目(QN2017021);河北省卫生与计划生育委员会资助项目(20180702);河北省高等学校科学技术研究项目(Z2014063)

Effects of TNF-α on sodium channel in cortical neurons of rats

ZHANG Weiwei, ZHANG Han, CAO Fang, MU Shumei, KANG Xianjiang

College of Life Sciences, Hebei University, Baoding 071002, China

Received:2017-10-25 Online:2018-05-25 Published:2018-05-25

摘要/Abstract

摘要： 以原代培养的胎鼠皮层神经元为材料,使用全细胞膜片钳技术,研究了10 ng/mL与50 ng/mL的肿瘤坏死因子(tumor necrosis factor-α,TNF-α)分别处理6 h和24 h后对皮层神经元电压依赖性钠电流的影响.结果显示,50 ng/mL的TNF-α对钠电流具有显著抑制作用,而对钠通道的激活性质与失活性质没有影响.

关键词: 肿瘤坏死因子α(TNF-α), 皮层神经元, 电压依赖性钠通道, 膜片钳记录

Abstract: Primary cultured fetal rat cortical neurons and whole cell patch clamp technique were used for investigating the effect of TNF-α on Na⁺ currents of cultured cortical neurons. The neurons were treated for 6 and 24 hours respectively by TNF-α(10 and 50 ng/mL). The results showed the inhibition of 50 ng/mL TNF-α on voltage-gated Na⁺ currents in cortical neurons of rats, but has no effect on the activation properties and the inactivation properties of sodium channel.

Key words: tumor necrosis factor -α, cortical neurons, voltage-dependent Na⁺ channel, Patch-clamp recording

中图分类号:

张伟伟, 张晗, 曹芳, 穆淑梅, 康现江. TNF-α对大鼠皮层神经元钠通道电流的影响[J]. 河北大学学报(自然科学版), 2018, 38(3): 278-283.

ZHANG Weiwei, ZHANG Han, CAO Fang, MU Shumei, KANG Xianjiang. Effects of TNF-α on sodium channel in cortical neurons of rats[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(3): 278-283.

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TNF-α对大鼠皮层神经元钠通道电流的影响

Effects of TNF-α on sodium channel in cortical neurons of rats

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