花生凝集素受体在中华绒螯蟹生精细胞的分布

doi:10.3969/j.issn.1000-1565.2019.03.010

河北大学学报(自然科学版) ›› 2019, Vol. 39 ›› Issue (3): 288-292.DOI: 10.3969/j.issn.1000-1565.2019.03.010

花生凝集素受体在中华绒螯蟹生精细胞的分布

杜锦梅,李路,马志芳,穆淑梅,康现江

收稿日期:2018-09-20 出版日期:2019-05-25 发布日期:2019-05-25
通讯作者: 穆淑梅(1972—),女,河北保定人,河北大学副教授,主要从事细胞发育分化及毒理学研究.E-mail:shumeimu@126.com康现江(1964—),男,河北邯郸人,河北大学教授,主要从事细胞发育分化及其调控研究.E-mail:xjkang218@126.com
作者简介:杜锦梅(1992—),女,山西吕梁人,河北大学在读硕士研究生,主要从事细胞发育分化及其调控研究. E-mail:1501542063@qq.com
基金资助:
国家自然科学基金资助项目(31572269;31272309)

Distribution of peanut agglutinin receptors in spermatogenic cells of Eriocheir sinensis

DU Jinmei,LI Lu,MA Zhifang,MU Shumei,KANG Xianjiang

College of Life Sciences, Hebei University, Baoding 071002, China

Received:2018-09-20 Online:2019-05-25 Published:2019-05-25

摘要/Abstract

摘要： 以中华绒螯蟹为材料,应用凝集素印迹法检测中华绒螯蟹生精细胞中的花生凝集素受体.以凝集素组织化学技术为手段,采用生物素标记的花生凝集素对中华绒螯蟹精子发生过程中花生凝集素受体进行了标记,研究精子发生过程中花生凝集素受体的分布特征.结果表明,中华绒螯蟹生精细胞中的花生凝集素受体的分子质量为72 ku;精子发生过程中,不同的生精细胞膜有不同程度的阳性标记.精原细胞质膜上有较强的阳性标记,呈深棕色;精母细胞的质膜阳性标记减少;精细胞与精母细胞染色程度相似.PNA受体在生精细胞中分布,可能与生殖细胞发育以及生殖细胞之间或者生殖细胞与体细胞之间的信息传递有关.精子质膜、顶体区域也有阳性标记,这可能与精卵识别及结合等受精过程息息相关.

关键词: 中华绒螯蟹, 生精细胞, 花生凝集素受体

Abstract: In order to detect the distribution characteristics of peanut agglutinin(PNA)receptors in spermatogenic germ cells of Eriocheir sinensis, lectin-blotting and lectin-histochemistry were used in this study. The results showed that the molecular weight of PNA receptors was 72 ku. Based on the binding sites of the biotin-labeled PNA, positively stained cells could be observed and recognized as surface receptors present in all types of spermatogenic cells. But, there were different degrees of PNA-postive singals emerged on these cells’ membrane. On spermatogonial cell surface, positive staining was strong, in dark brown. Positive signals were similar and weakened on spermatocytes and spermatids. The distribution characteristics of PNA receptors on spermatogenic cells may be related to the development of germ cells as well as the information transmission between germ cells or between germ cells and somatic cells. There were also positive signals on sperm surface and in its acrosome. These PNA receptors may be closely related to the fertilization, such as sperm-oocyte recognition and binding.

Key words: Eriocheir sinensis, spermatogenic cells, peanut agglutinin receptor

中图分类号:

Q954.43

杜锦梅,李路,马志芳,穆淑梅,康现江. 花生凝集素受体在中华绒螯蟹生精细胞的分布[J]. 河北大学学报(自然科学版), 2019, 39(3): 288-292.

DU Jinmei,LI Lu,MA Zhifang,MU Shumei,KANG Xianjiang. Distribution of peanut agglutinin receptors in spermatogenic cells of Eriocheir sinensis[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(3): 288-292.

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花生凝集素受体在中华绒螯蟹生精细胞的分布

Distribution of peanut agglutinin receptors in spermatogenic cells of Eriocheir sinensis

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