白洋淀不同区域荷叶品质和抗氧化性差异

doi:10.3969/j.issn.1000-1565.2020.05.011

摘要/Abstract

摘要： 为探究白洋淀不同区域荷叶品质和抗氧化性的差异,对白洋淀7个有代表性采样点的荷叶进行了研究.实验结果表明,白洋淀7个采样点荷叶的品质和抗氧化性差异明显:杨庄子和烧车淀采样点的荷叶活性成分含量较高;烧车淀采样点的荷叶蛋白和维生素C含量较高,郭里口采样点的荷叶可溶性糖和蛋白含量较高;而大田庄采样点的荷叶具有较强的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)和O^-₂自由基清除活性.因此,综合分析表明,烧车淀和大田庄采样点的荷叶均具有较高的品质和较强的抗氧化性.

关键词: 白洋淀, 荷叶, 活性成分, 营养成分, 抗氧化性

Abstract: In order to explore the differences in quality and antioxidant activities of lotus leaves in different regions of Baiyangdian Lake, this work studied the lotus leaves in seven representative regions of Baiyangdian Lake.The results demonstrated that there were significant differences in the physiological traits,active and nutritional ingredients and antioxidant activities of the lotus leaves in different regions of Baiyangdian Lake: The lotus leaves in the Yangzhuangzi and Shaochedian regions had higher contents of active ingredient;The lotus leaves in the Shaochedian region had higher contents of protein and vitamin C,and the soluble sugar and protein contents in lotus leaves were higher in Guolikou region than that in the other regions;The lotus leaves in the Datianzhuang region had higher activities in 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging and O^-₂ radical scavenging activity.Comprehensive analysis revealed that the lotus leaves in the Shaochedian and Datianzhuang sampling points had higher qualities and stonger antioxidant activities.This results of this study provided a theoretical basis for selecting the optimal picking region of lotus leaves in Baiyangdian Lake.- DOI:10.3969/j.issn.1000-1565.2020.05.011白洋淀不同区域荷叶品质和抗氧化性差异郭春延¹,楚建周^1,2,薛晶晶¹,郭臣臣¹,申雨肖¹,姚晓芹^1,2,3(1.河北大学生命科学学院,河北保定 071002;2.河北大学生命科学与绿色发展研究院,河北保定 071002;3.河北省湿地生态与保护重点实验室(筹),河北衡水 053000)摘要:为探究白洋淀不同区域荷叶品质和抗氧化性的差异,对白洋淀7个有代表性采样点的荷叶进行了研究.实验结果表明,白洋淀7个采样点荷叶的品质和抗氧化性差异明显:杨庄子和烧车淀采样点的荷叶活性成分含量较高;烧车淀采样点的荷叶蛋白和维生素C含量较高,郭里口采样点的荷叶可溶性糖和蛋白含量较高;而大田庄采样点的荷叶具有较强的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)和O^-₂自由基清除活性.因此,综合分析表明,烧车淀和大田庄采样点的荷叶均具有较高的品质和较强的抗氧化性.关键词:白洋淀;荷叶;活性成分;营养成分;抗氧化性中图分类号:R282 文献标志码:A 文章编号:1000-1565(2020)05-0527-09Qualities and antioxidant activities of lotus leaves in different regions of Baiyangdian LakeGUO Chunyan¹, CHU Jianzhou^1,2, XUE Jingjing¹, GUO Chenchen¹, SHEN Yuxiao¹, YAO Xiaoqin^1,2,3(1.College of Life Sciences, Hebei University, Baoding 071002, China; 2.Institute of Life Science and Green Development, Hebei University, Baoding 071002, China; 3. Hebei Key Laboratory of Wetland Ecology and Conservation,Hengshui 053000, China)Abstract: In order to explore the differences in quality and antioxidant activities of lotus leaves in different regions of Baiyangdian Lake, this work studied the lotus leaves in seven representative regions of Baiyangdian Lake.The results demonstrated that there were significant differences in the physiological traits,active and nutritional ingredients and antioxidant activities of the lotus leaves in different regions of Baiyangdian Lake: The lotus leaves in the Yangzhuangzi and Shaochedian regions had higher contents of active ingredient;The lotus leaves in the Shaochedian region had higher contents of protein and vitamin C,and the soluble sugar and protein contents in lotus leaves were higher in Guolikou region than that in the other regions;The lotus leaves in the Datianzhuang region had higher activities in 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging and O^-₂ radical scavenging activity.Comprehensive analysis revealed that the lotus leaves in the Shaochedian and Datianzhuang sampling points had higher qualities and stonger antioxidant activities.This results of this study provided a theoretical basis for selecting the optimal picking region of lotus leaves in Baiyangdian Lake.- 收稿日期:2019-04-21 基金项目: 国家自然科学基金资助项目(31300321);河北省自然科学基金资助项目(C2012201080);河北省湿地生态与保护重点实验室(筹)开放基金资助项目(hklk201904) 第一作者: 郭春延(1991—),女,河北邢台人,河北大学在读硕士研究生.E-mail: 1076568696@qq.com 通信作者: 姚晓芹(1978—),女,河北邯郸人,河北大学教授,主要从事植物生理生态学研究. E-mail: yaoxiao301@126.com第5期郭春延等:白洋淀不同区域荷叶品质和抗氧化性差异

Key words: Baiyangdian Lake, lotus leaf, active ingredients, nutritional ingredients, antioxidant activity

中图分类号:

R282

郭春延,楚建周,薛晶晶,郭臣臣,申雨肖,姚晓芹. 白洋淀不同区域荷叶品质和抗氧化性差异[J]. 河北大学学报(自然科学版), 2020, 40(5): 527-535.

GUO Chunyan, CHU Jianzhou, XUE Jingjing, GUO Chenchen, SHEN Yuxiao, YAO Xiaoqin. Qualities and antioxidant activities of lotus leaves in different regions of Baiyangdian Lake[J]. Journal of Hebei University (Natural Science Edition), 2020, 40(5): 527-535.

参考文献

[1] HU J H, PAN L, LIU H G, et al.Comparative analysis of genetic diversity in sacred lotus(Nelumbo nucifera Gaertn.)using AFLP and SSR markers[J].Mol Biol Rep, 2012, 39(4): 3637-3647. DOI:10.1007/s11033-011-1138-y.
[2] KATO E, INAGAKI Y, KAWABATA J.Higenamine 4'-O-β-d-glucoside in the lotus plumule induces glucose uptake of L6 cells through β2-adrenergic receptor[J]. Bioorg Med Chem, 2015, 23(13): 3317-3321. DOI:10.1016/j.bmc.2015.04.054.
[3] CHEN S, FANG L C, XI H F,et al.Simultaneous qualitative assessment and quantitative analysis of flavonoids in various tissues of lotus(Nelumbo nucifera)using high performance liquid chromatography coupled with triple quad mass spectrometry[J]. Anal Chim Acta, 2012, 724: 127-135. DOI:10.1016/j.aca.2012.02.051.
[4] DENG J, CHEN S, YIN X J, et al. Systematic qualitative and quantitative assessment of anthocyanins, flavones and flavonols in the petals of 108 lotus(Nelumbo nucifera)cultivars[J]. Food Chem, 2013, 139(1/2/3/4): 307-312. DOI:10.1016/j.foodchem.2013.02.010.
[5] HUANG B, BAN X Q, HE J S, et al. Hepatoprotective and antioxidant activity of ethanolic extracts of edible lotus(Nelumbo nucifera Gaertn.)leaves[J]. Food Chem, 2010, 120(3): 873-878. DOI:10.1016/j.foodchem.2009.11.020.
[6] 侯芳洁,李英,宋军娜,等.河北省涉县不同采样点柴胡药材中柴胡皂苷的含量比较[J].时珍国医国药,2016,27(3): 720-722.DOI: 10.3969/j.iss.1008-0805.2016.03.077.
[7] 孙莹.不同生境条件下蓝花楹生长发育差异研究[D].雅安: 四川农业大学,2016.
[8] 魏华东.白洋淀荷叶中营养元素和重金属元素含量分析[D].保定: 河北大学,2017.
[9] LI X C. Improved pyrogallol autoxidation method: a reliable and cheap superoxide-scavenging assay suitable for all antioxidants[J]. J Agric Food Chem, 2012, 60(25): 6418-6424. DOI:10.1021/jf204970r.
[10] 华智锐,李小玲.盐旱交叉胁迫对小麦幼苗渗透调节能力的影响[J].山西农业科学,2017,45(2): 166-171.DOI: 10.3969/j.issn.1002-2481.2017.02.05.
[11] PENG C L, LIN Z F, LIN G Z, et al. The anti-photooxidation of anthocyanins-rich leaves of a purple rice cultivar[J]. Sci China, C, Life Sci, 2006, 49(6): 543-551. DOI: 10.1007/s11427-006-2022-1.
[12] LICHTENTHALER H K. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes[J]. Methods in Enzymology Elsevier, 1987, 148: 350-382. DOI:10.1016/0076-6879(87)48036-1.
[13] 王蕾,赵永娟,张媛媛,等.半夏生物碱含量测定及止呕研究[J].中国药理学通报,2005,21(7): 864-867.DOI: 10.3321/j.issn:1001-1978.2005.07.024.
[14] 何书美,刘敬兰.茶叶中总黄酮含量测定方法的研究[J].分析化学,2007,35(9): 1365-1368.DOI: 10.3321/j.issn:0253-3820.2007.09.028.
[15] 张以顺,黄霞,陈云凤.植物生理学实验教程[M].北京: 高等教育出版社,2009.
[16] 张颖,张立木,王立秋,等.不同产地枸杞子中维生素C含量测定[J].中国医院药学杂志,2004,24(8): 500-501.DOI: 10.3321/ji.ssn:1001-5213.2004.08.032.
[17] MACHADO R A R, FERRIERI A P, ROBERT C A, et al.Leaf-herbivore attack reduces carbon reserves and regrowth from the roots via jasmonate and auxin signaling[J]. New Phytol, 2013, 200(4): 1234-1246. DOI:10.1111/nph.12438.
[18] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976, 72: 248-254. DOI:10.1006/abio.1976.9999.
[19] MOHSEN S M, AMMAR A S M. Total phenolic contents and antioxidant activity of corn tassel extracts[J]. Food Chem, 2009, 112(3): 595-598. DOI:10.1016/j.foodchem.2008.06.014.
[20] CHEN R Z, LI Y, DONG H, et al.Optimization of ultrasonic extraction process of polysaccharides from Ornithogalum Caudatum Ait and evaluation of its biological activities[J]. Ultrason Sonochemistry, 2012, 19(6): 1160-1168. DOI:10.1016/j.ultsonch.2012.03.008.
[21] YU J, TANG X X, ZHANG P Y, et al.Effects of CO₂ enrichment on photosynthesis, lipid peroxidation and activities of antioxidative enzymes of Platymonas subcordiformis subjected to UV-B radiation stress[J]. Acta Botanica Sinica, 2004, 46(6): 682-690.
[22] 刁淑清,谢志明,韩月明.白城地区两种不同类型土壤对糜子丙二醛含量及产量性状的影响[J].白城师范学院学报,2015,29(11): 46-48,65.
[23] 司超.紫外线-B对釆后药用菊花花序生理特性和品质的影响及其机制[D].保定: 河北大学,2015.
[24] 莫秋华,耿耘,杨洋,等.紫山药花色素苷稳定性研究[J].食品科技,2018,43(4): 218-222.DOI:10.13684/j.cnki.spkj.2018.04.040.
[25] SUN X L, XU Y F, MA L Y, et al.A review of acclimation of photosynthetic pigment composition in plant leaves to shade environment[J]. Chinese Journal of Plant Ecology, 2010, 34(8): 989-999. DOI: http://dx.doi.org/.
[26] 刘晓庚,袁磊,高梅,等.类胡萝卜素清除自由基的动力学及体外模拟消化对清除率的影响[J].食品科学,2016,37(11): 65-73.DOI:10.7506/spkx1002-6630-201611012.
[27] 朱运钦,乔改梅,王志强.植物类胡萝卜素代谢调控的研究进展[J].分子植物育种,2016,14(2): 471-474. DOI:10.13271/j.mpb.014.000471.
[28] BREITHAUPT D E, BAMEDI A. Carotenoids and carotenoid esters in potatoes(Solanum tuberosum L.): new insights into an ancient vegetable[J]. J Agric Food Chem, 2002, 50(24): 7175-7181. DOI:10.1021/jf0257953.
[29] 刘秀香.松嫩平原两种生境芦苇叶片光合色素的时空动态[D].长春: 东北师范大学,2013.
[30] 黄开颜,张志国.荷叶研究概况[J].中国药业,2008,17(11): 77-79.
[31] MANTHEY J A, GROHMANN K, GUTHRIE N. Biological properties of citrus flavonoids pertaining to cancer and inflammation[J]. Curr Med Chem, 2001, 8(2): 135-153. DOI:10.2174/0929867013373723.
[32] KIM J, LEE I, SEO J, et al. Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3-L1 cells[J]. Phytother Res, 2010,24(10): 1543-1548. DOI:10.1002/ptr.3186.
[33] 向前胜,王宁,赵越,等.青海省3种小檗不同地区、不同部位小檗碱含量的比较研究[J].西南农业学报,2016,29(1): 54-58.DOI:10.16213/j.cnki.scjas.2016.01.011.
[34] CHO J S, LEE J S, KIM J W. Distribution of Phragmites australis communities with different habitat salinity[J]. J Coast Res,2017, 33(5): 1210-1216. DOI:10.2112/jcoastres-d-16-00065.1.
[35] 葛文.山东烟台地区土壤地球化学环境与优质苹果生产的适应性评价[D].武汉: 中国地质大学,2013.
[36] 马芝丽,马亚洁,黄文.不同产区干莲子营养成分及加工特性分析[J].食品工业科技,2019,40(1): 96-100.DOI:10.13386/j.issn1002-0306.2019.01.018.
[37] 王晓雅.安康宁陕地区中蜂蜜抗氧化性研究[J].陕西农业科学,2018,64(3): 57-60.
[38] 刘琴,张薇娜,朱媛媛,等.不同产地苦荞籽粒中多酚的组成、分布及抗氧化性比较[J].中国农业科学,2014,47(14): 2840-2852.DOI: 10.3864/j.issn.0578-1752.2014.14.014.
[39] 曲晶升,万晴姣.贵州不同地区辣椒抗氧化活性研究[J].中国粮油学报,2009,24(2): 113-115.