胶体金免疫层析法检测3种有机磷农药残留

doi:10.3969/j.issn.1000-1565.2025.05.006

河北大学学报(自然科学版) ›› 2025, Vol. 45 ›› Issue (5): 497-506.DOI: 10.3969/j.issn.1000-1565.2025.05.006

胶体金免疫层析法检测3种有机磷农药残留

柴维娜,曹子玥,谭皓元,董江雪,苏明

收稿日期:2025-02-28 发布日期:2025-09-18
通讯作者: 苏明(1982—)
作者简介:柴维娜(1997—),女,河北大学在读硕士研究生,主要从事侧向流免疫层析试纸卡研制.
E-mail:cwn786177470@163.com
基金资助:
国家自然科学基金项目(32401191);河北省自然科学基金项目(B2023201032);河北省创新能力提升计划项目(225A4802D);河北大学科研创新团队计划(IT2023A03);河北省教育厅科研项目(JZX2024019)

Determination of three organophosphorus pesticide residues by colloidal gold immunochromatography

CHAI Weina, CAO Ziyue,TAN Haoyuan,DONG Jiangxue, SU Ming

Hebei Key Laboratory of Analytical Science and Technology, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China

Received:2025-02-28 Published:2025-09-18

摘要/Abstract

摘要： 为检测有机磷农药在环境及食品中的残留,建立了一种基于胶体金的比色免疫层析检测方法.在最优实验条件下,建立了标准曲线.该检测方法对杀螟硫磷、甲基对硫磷和对硫磷3种有机磷农药的检出限分别可达0.94、0.32、0.03 ng/mL,适用于有机磷农药残留的初步筛选.对桃子样品进行加标回收,结果显示:杀螟硫磷的回收率为88%~113%,相对标准偏差≤10%;甲基对硫磷的回收率为107%~118%,相对标准偏差≤5%;对硫磷的回收率为106%~113%,相对标准偏差≤5%.实验结果表明,本方法具有较高的准确性和可靠性,且不易受样品基质的影响,能够准确检测桃汁中的有机磷农药残留.

关键词: 胶体金, 侧流免疫层析, 有机磷农药

Abstract: In this study, a colorimetric immunochromatography method based on colloidal gold was developed. Under optimal experimental conditions, the standard curve was established. The detection limits of this method for the three organophosphorus pesticides were 0.94, 0.32, 0.03 ng/mL, respectively, and the method was suitable for the preliminary screening of organophosphorus pesticide residues.The recovery of peach samples by standard addition showed that the recoveries rate of fenitrothion were 88%—113%, and the relative standard deviation were less than 10%. The recoveries rate of parathion methyl ranged were 107%—118%, and the relative standard deviation were less than 5%. The recoveries rate of parathion were 106%—113%, and the relative standard deviation were less than 5%.The experimental results showed that the method had high accuracy and reliability, and was not easily affected by the sample matrix, and could accurately detect organophosphorus pesticide residues in peach juice.

Key words: colloidal gold, lateral flow immunochromatography, organophosphorus pesticide

中图分类号:

O657.7
X5

柴维娜,曹子玥,谭皓元,董江雪,苏明. 胶体金免疫层析法检测3种有机磷农药残留[J]. 河北大学学报(自然科学版), 2025, 45(5): 497-506.

CHAI Weina, CAO Ziyue,TAN Haoyuan,DONG Jiangxue, SU Ming. Determination of three organophosphorus pesticide residues by colloidal gold immunochromatography[J]. Journal of Hebei University(Natural Science Edition), 2025, 45(5): 497-506.

参考文献

[1] WANG X Q, WANG J, HU B B,et al. Rapid determination of organophosphorus insecticides residue in fruit juice by a fluorescence sensor based on carbon quantum dots[J].J Food Compos Anal, 2024, 136: 106816. DOI:10.1016/j.jfca.2024.106816.
[2] PREMALATHA N, REX P. A comprehensive review on photocatalytic degradation of organophosphorus pesticide using ZnO coupled photocatalysts[J]. Desalin Water Treat,2024,320: 100753.DOI:10.1016/j.dwt.2024.100753.
[3] WANG K, DONG F X, PAN X L, et al. Quantitative screening of organophosphorus and organochlorine pesticides in water and soil using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry[J].J Chromatogr Open,2024, 5: 100140.DOI:10.1016/j.jcoa.2024.100140.
[4] AL-MALIKI A M J, MASROURNIA M, KHOSHNOOD R S, et al. Dispersive micro solid-phase extraction with bimetallic Ni, Zn-MOF sorbent and gas chromatography method for organophosphorus pesticide determination in environmental water samples[J]. Microchem J, 2024, 205: 111259.DOI:10.1016/j.microc.2024.111259.
[5] JAFARI Z, GHANI M. Magnetic carbonized cellulose-MIL 101(Fe)composite as a sorbent for magnetic solid phase extraction of selected organophosphorus pesticides combined with high performance liquid chromatography-ultraviolet detection[J]. Talanta Open, 2023, 7: 100212.DOI:10.1016/j.talo.2023.100212.
[6] LI P, MENG J P, ZHANG C, et al. Mass spectrometry detection of organophosphorus pesticide adducts on butyrylcholinesterase and albumin[J]. J Chromatogr B, 2024, 1243: 124195.DOI:10.1016/j.jchromb.2024.124195.
[7] ZHANG S L, YOU Q, ZHUO X C, et al. Rapid and simple determination of organophosphorus pesticides in urine using polydopamine-modified monolithic spin column extraction combined with liquid chromatography-mass spectrometry[J]. J Chromatogr A, 2023, 1696: 463959.DOI:10.1016/j.chroma.2023.463959.
[8] RAZA S H A, HUANG Z X, PANG Y M, et al. Rapid screening of prednisolone acetate adulterants in health foods using colloidal gold immunochromatographic assay[J]. Chem Phys Lipids, 2024, 265: 105447.DOI:10.1016/j.chemphyslip.2024.105447.
[9] WANG Y D, FU Y W, MIAO K, et al. A colloidal gold immunochromatographic method for rapid screening of imidacloprid residues in Chinese herbal medicines[J]. J Chromatogr B, 2024, 1244: 124240.DOI:10.1016/j.jchromb.2024.124240.
[10] SUN J D, WANG Y Y, ZHANG T, et al. Development of recombinant antibody and application of colloidal gold immunochromatography assay for rapid detection of zearalenone[J]. Food Biosci, 2025, 63: 105714.DOI:10.1016/j.fbio.2024.105714.
[11] 王颖,刘军,汪震宇,等.新型烟碱胶体金免疫层析快速检测法的建立[J].烟草科技,2025,58(2):11-19.DOI:10.16135/j.issn1002-0861.2024.0443.
[12] 方琪珑,李懿绮,江伊徽,等.神奇的农残检测试纸条——用于有机磷农药残留检测的核酸适配体侧流层析试纸条[J].大学化学,2024,39(5):80-89.DOI: 10.3866/PKU.DXHX202310004.
[13] 胡静,郭逸蓉,梁晓,等.菊酯类农药广谱型免疫层析试纸条的研究及应用[J].分析化学,2016,44(12):1900-1906.DOI:10.11895/j.issn.0253-3820.160428.
[14] 董晓涵,翟璐,黄雅丽,等.抗丁香酚单克隆抗体的研制及胶体金试纸条检测方法的建立[J].核农学报,2025,39(2):339-350. DOI:10.11869/j.issn.1000-8551.2025.02.0339.
[15] 薛洽升.胶体金免疫层析技术在食品快速检测中的应用[J].食品界,2025(1):29-31.
[16] LI G Z, GUAN F K, ZHAO S C, et al. Immediate, sensitive and specific time-resolved fluorescent immunoassay strips based on immune competition for the detection of procymidone in vegetables[J]. Food Contr, 2023, 147: 109569. DOI:10.1016/j.foodcont.2022.109569.
[17] 成永强, 赵亚青, 高舒心, 等. 基于金纳米粒子的催化发夹DNA组装检测microRNA[J]. 河北大学学报(自然科学版), 2018,38(2): 147-153. DOI:10.3969/j.issn.1000-1565.2018.02.006.
[18] SARAIVA S M, DE OLIVEIRA J F. Control of particle size in the preparation of colloidal gold[J]. J Dispers Sci Technol, 2002, 23(6): 837-844. DOI:10.1081/dis-120015980.
[19] LI X F, LIU L Q, SONG S S, et al. Colloidal gold immunochromatographic assay for the detection of total aflatoxins in cereals[J]. Food Chem, 2025, 472: 142877.DOI:10.1016/j.foodchem.2025.142877.
[20] 定翔雁,魏梓玉,谢迪佳,等. 基于胶体金多联卡的农产品质量安全监管研究[J].食品安全导刊,2025(1):21-23.DOI:10.16043/j.cnki.cfs.2025.01.030.
[21] CHEN R S, YUAN J C, LUO C L, et al. Monoclonal antibody based colloidal gold immunochromatographic assay for the visual and rapid screening of profenofos.[J].Environ Pollut,2024,361:124848.DOI:10.1016/j.envpol.2024.124848.
[22] GALÁN-CANO F, LUCENA R, CÁRDENAS S, et al. Dispersive micro-solid phase extraction with ionic liquid-modified silica for the determination of organophosphate pesticides in water by ultra performance liquid chromatography[J].Microchem J,2013,106: 311-317.DOI:10.1016/j.microc.2012.08.016.
[23] PRAPAMONTOL T, SUTAN K, LAOYANG S,et al. Cross validation of gas chromatography-flame photometric detection and gas chromatography-mass spectrometry methods for measuring dialkylphosphate metabolites of organophosphate pesticides in human urine[J].Int J Hyg Environ Health,2014,217(4/5): 554-566.DOI:10.1016/j.ijheh.2013.10.005.
[24] ZHENG Q Q, CHEN Y, FAN K, et al. Exploring pralidoxime chloride as a universal electrochemical probe for organophosphorus pesticides detection[J].Anal Chim Acta, 2017, 982: 78-83. DOI:10.1016/j.aca.2017.06.006.
[25] 邵丹.常规农药胶体金快速检测服务农产品产地准出——常州市胶体金智能化实践与探索[J].新农民,2024(36):53-55. (

胶体金免疫层析法检测3种有机磷农药残留

Determination of three organophosphorus pesticide residues by colloidal gold immunochromatography

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	郑佳妍,周丽岩,韩晓利,李昳晴,王庭欣. 辣椒、花椒中罗丹明B免疫层析分析[J]. 河北大学学报(自然科学版), 2021, 41(6): 682-689.
[2]	解瑶,刘中成,张艳芬,赵丽健,刘利鹏. 适于标记核酸适配子胶体金的制备及性能[J]. 河北大学学报(自然科学版), 2014, 34(3): 284-289.