蚯蚓冻干粉对高血脂小鼠胆固醇逆转运
相关基因的调节作用

doi:10.3969/j.issn.1000-1565.2015.02.009

河北大学学报(自然科学版) ›› 2015, Vol. 35 ›› Issue (2): 159-164.DOI: 10.3969/j.issn.1000-1565.2015.02.009

蚯蚓冻干粉对高血脂小鼠胆固醇逆转运相关基因的调节作用

武金霞¹，武建¹，隋思强¹，吴红月¹，张贺迎²

1. 河北大学生命科学学院，河北保定 071002；2. 河北大学生物工程技术研究中心，河北保定 071002

收稿日期:2014-09-25 修回日期:2014-11-25 出版日期:2015-03-25 发布日期:2015-03-25
通讯作者: 张贺迎
作者简介:张贺迎(1966-)，男，河北大成人，河北大学教授，主要从事生化药物及基因工程药物研究
基金资助:
河北省生物工程重点学科资助

Regulation of Earthworm Lyophilized Powder on Reverse Cholesterol Transport Genes Expression in Hyperlipidemic Mice

WU Jinxia¹, WU Jian¹,SUI Siqiang¹, WU Hongyue¹, ZHANG Heying²

1. College of Life Sciences, Hebei University, Baoding 071002,China; 2. Research Center of Bioengineering, Hebei University, Baoding 071002, China

Received:2014-09-25 Revised:2014-11-25 Online:2015-03-25 Published:2015-03-25
Contact: ZHANG Heying

摘要/Abstract

摘要： 以高脂饲料饲喂昆明种小鼠构建高血脂小鼠模型，灌胃给予高、中、低剂量（0.4，0.2，0.1 g.kg-1）蚯蚓冻干粉，每天1次，同时饲喂高脂饲料，以仅饲喂高脂饲料组小鼠为对照，共饲养10周.提取小鼠肝脏总RNA，应用半定量RT-PCR技术检测实验组与对照组小鼠胆固醇逆转运相关基因卵磷脂胆固醇酰基转移酶（Lecithin cholesterol acyltransferase, LCAT）和脂蛋白酯酶（Lipoprotein Lipase, LPL）mRNA表达水平.结果表明，蚯蚓冻干粉极显著提高实验组小鼠LCAT mRNA表达水平（P<0.01），显著提高LPL mRNA表达水平（P<0.05），提示这是蚯蚓冻干粉能够降低高血脂小鼠血浆胆固醇水平的原因之一

关键词: 蚯蚓冻干粉, 高血脂症, 胆固醇代谢, 卵磷脂胆固醇酰基转移酶, 脂蛋白酯酶

Abstract: Kunming mice were fed with high-level lipid feeds to establish the hyperlipidemia mice models. The mice were treated with earthworm lyophilized powder at dosages of 0.4, 0.2 and 0.1 g/kg once a day and were fed with high-level lipid feeds at the same time for 10 weeks, the mice fed only with high-level lipid feeds were as the control. The total RNA of liver was extracted, and the changes of liver Lecithin cholesterol acyltransferase and Lipoprotein Lipase gene expression were analyzed by RT- PCR. The results indicated that earthworm lyophilized powder up-regulated Lecithin cholesterol acyltransferase and Lipoprotein Lipase mRNA expression markedly, suggesting it was one of the reasons for reducing serum cholesterol in hyperlipidemia mice of earthworm lyophilized powder.

Key words: earthworm lyophilized powder, hyperlipidemia, cholesterol metabolism, lecithin cholesterol acyltransferase, lipoprotein lipase

中图分类号:

Q507

武金霞，武建，隋思强，吴红月，张贺迎. 蚯蚓冻干粉对高血脂小鼠胆固醇逆转运相关基因的调节作用[J]. 河北大学学报(自然科学版), 2015, 35(2): 159-164.

WU Jinxia, WU Jian,SUI Siqiang, WU Hongyue, ZHANG Heying. Regulation of Earthworm Lyophilized Powder on Reverse Cholesterol Transport Genes Expression in Hyperlipidemic Mice[J]. Journal of Hebei University (Natural Science Edition), 2015, 35(2): 159-164.

参考文献

[1]单彪，武金霞，张瑞英，等.地龙的药理作用研究进展. 医学研究与教育, 2009, 26 (6): 77-80. SHAN Biao,WU JinXia,ZHANG RuiYing,et al. Research progress on pharmacological activities of the DiLong.Medical Research and Education, [2]韩立军. 蚯蚓组织提取物的抗菌、抗肿瘤及免疫增强活性研究. 河北农业大学硕士生论文. 2007.HAN LiJun.Studies on the anti-biotic,anti-tumor, and immunology enhancement activities of earthworm tissue extracts. Master dissertation of Agriculture University of HeBei, [3] Tu Z, Han X, Wang X, et al. Hou Y, Protective effects of CVPM on vascular endothelium in rats fed cholesterol diet. Clin Chim Acta. 2003，333(1):85-90. [4]武金霞,单彪,习洋,等.蚯蚓冻干粉的抗氧化作用.河北大学学报（自然科学版），2010，30 （1）73-77. WU J inxia,SHAN Biao, XI Yang, et al. Anti-oxidation Activities of Earthworm Lyophilized Powder. Journal of Hebei University (Natural Science Edition), 2010，30（1）73-77. [5]甄兴航.蚯蚓冻干粉调节小鼠血脂水平及机理的研究.河北大学硕士论文.2009. Study on the regulation bloods lipids and mechanism in mice of earthworm lyophilized powder. Master dissertation of Hebei University. [6]武金霞，刘雪英，单彪，等. 蚯蚓冻干粉对高血脂小鼠胆固醇7α-羟化酶基因的调节作用.中国医药工业杂志.2011, 42(4):262-264. WU Jinxia, LIU Xueying, SHAN Biao, et al. Regulation of the Lyophilized Powder of Earthworm on Cholesterol 7α-Hydroxylase Gene in Hyperlipidemic Mice Chinese Journal of Pharmaceuticals 2011, 42(4): 262-264. [7]武金霞,田志鹏,单彪等.蚯蚓冻干粉对高血脂症小鼠载脂蛋白ＡＩ和载脂蛋白Ｂ基因表达的影响.中国药理学通报， 2010，26(12):1649～52. WU Jinxia, TI AN Zhipeng, SHAN Biao,et al. Effects of earthworm lyophilized powder on ApoAI and ApoBgene expression in hyperlipidemic mice. Chinese Pharmacological Bulletin,2010,26( 12) : 1649~ 52. [8]王晓黎,王冬冬,张锦.卵磷脂胆固醇酰基转移酶缺陷症LCATG179R 突变的初步研究. 中国医科大学学报，2012,41(5): 405-408. WANG Xiao li,WANG Dong dong,ZHANG Jin. One Novel Mutation（G179R ） in LCAT Can Cause the Inactivity of the Enzyme. Journal of China Medical University, 2012,41(5): 405-408. [9] 徐俊楠.肝脏脂蛋白酯酶LPL的代谢调节功能与机制研究. 第二军医大学硕士论文， 2013.Studies on the role of liver lipoprotein lipase in lipid metabolism and mechanism. Master dissertation of the second military medical university. [10]Colombero C, Catano G, Rocco CA,Role of Three Lipoprotein Lipase Variants in Triglycerides in Children Receiving HAART. Pediatr Infect Dis J. 2014 Jul 1. [Epub ahead of print]. [11]陈文祥.卵磷脂胆固醇酰基转移酶活性与心血管病危险.实用医院临床杂志，2012，9 （3 ）：4-7. CHEN Wenxiang. Activity and cardiovascular risk of lecithin cholesterol acyltransferase. Practical Journal of Clinical Medicine, 2012，9 （3 ）：4-7. [12] Aguilar-Espinosa SL, Mendoza-Espinosa P, Delgado-Coello B, et al.Lecithin cholesterol acyltransferase (LCAT) activity in the presence of Apo-AI-derived peptides exposed to disorder-order conformational transitions. Biochem Biophys Res Commun. 2013,441(2):469-75. [13] 梁国栋.医学分子生物学实验技术[M]. 北京：科学出版社，2001:133-135.LIANG Guodong. Techniques of medical molecular biology. Beijing :Science Press, 2001:133-135.

蚯蚓冻干粉对高血脂小鼠胆固醇逆转运相关基因的调节作用

Regulation of Earthworm Lyophilized Powder on Reverse Cholesterol Transport Genes Expression in Hyperlipidemic Mice

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价

蚯蚓冻干粉对高血脂小鼠胆固醇逆转运 相关基因的调节作用

Regulation of Earthworm Lyophilized Powder on Reverse Cholesterol Transport Genes Expression in Hyperlipidemic Mice

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价

蚯蚓冻干粉对高血脂小鼠胆固醇逆转运相关基因的调节作用